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Equipment & Software

Instrumentation

• Two ABI 7900HTs with 96- and 384-well exchangeable blocks. One 7900 is available with high-throughput automation accessory, Fast PCR Block, and TLDA (Taqman Low Density Array) block.
• One Roche 480 LightCycler with 96- and 384-well exchangeable blocks. Fast PCR compatible.
• Sequenom Compact MassARRAY System
• Nanodrop 1000 spectrophotometer
• Agilent 2100 Bioanalyzer
• Two MJ Research tetrad DNA engines with four independent PCR blocks.
• ABI 9600 PCR block with exchangeable 96- and 384-well blocks.
• Also available for evaluation by Cancer Center members is an ABI 6100 semi-automated nucleic acid purification instrument that is capable of purifying 96 samples (up to 2 million cells/ well) from cells (from culture or sorting) to pure RNA in about 45 minutes.

Software

• Gene Codes Sequencher 4.7
• ABI Primer Express 2.0 and 3.0
• ABI SDS vs 2.1 and 2.3
• ABI SeqScape 2.5
• Softgenetics Mutation Surveyor
• Sequenom Assay Design 3.1 (SNP Genotyping)
• Sequenom Typer 3.4 and 4.0 (SNP Genotyping Analysis)
• Sequenom EpiDesigner (Methylation Experiment Design)
• Sequenom EpiTyper (Methylation Analysis)
• Sequenom QGE (Quantitative Gene Expression Analysis)