Thea D. Tlsty, PhD

University of South Florida, Tampa, FL, B.S., 1973, Zoology
Washington University, St. Louis, MO, Ph.D., 1980, Molecular Biology
Washington University, St. Louis, MO, 1976-1980, Predoctoral Fellow
Stanford University, Stanford, CA, 1981-1984, Postdoctoral Fellow
Stanford University, Stanford, CA, 1984-1985, Senior Research Associate

• 1974-1976
  Ph.D. Candidate, Dept. of Pathology, University of North Carolina, Chapel Hill (transferred)
• 1976-1980
  Pre-doctoral Fellow in Program for Cellular & Molecular Biology, Washington University, St. Louis, Missouri (advisor, Dr. M. Lieberman)
• 1980-1981
  Postdoctoral Fellow in Dept. of Microbiology & Immunology, Washington Univ., St. Louis, MO
• 1981-1985
  Postdoctoral Fellow/Senior Research Associate in Department of Biological Sciences, Stanford University, Stanford, California (advisor, Dr. R.T. Schimke)
• 1985-1994
  Assistant/Associate Professor of Pathology and Member: UNC Lineberger Comprehensive Cancer Center; University of North Carolina at Chapel Hill, North Carolina
• 1994-Present
  Professor of Pathology, Director of the Center for Translational Research in the Molecular Genetics of Cancer, Member: UCSF Comprehensive Cancer Center – Director for the Program in Cell Cycling and Signaling; Program in Biological Sciences (PIBS); Program in Biomedical Sciences (BMS), University of California at San Francisco, CA

• Tlsty TD, Albertini AM and Miller JH "Gene Amplification in the lac Region of Escherichia coli." Cell 37:217-224, 1984
• Tlsty TD, Margolin B and Lum K "Differences in the Rates of Gene Amplification in Non-tumorigenic and Tumorigenic Cell Lines as Measured by Luria-Delbrück Fluctuation Analysis." PNAS 86,9441-9445, 1989
• Tlsty TD "Normal diploid human and rodent cells lack a detectable frequency of gene amplification." PNAS 87:3132-3135, 1990
• Tlsty TD, White A and Sanchez J "Suppression of Gene Amplification in Human Cell Hybrids." Science 255:1425-1427, 1992
• Livingstone L, White A, Sprouse J, Livanos E and Tlsty TD "Altered Cell Cycle Arrest and Gene Amplification Potential Accompany Loss of Wild-Type p53." Cell 70:923-935, 1992
• Nigro JM, Aldape KD, Tlsty TD “Anchorage-dependent expression of p53 protein in primary human keratinocytes in culture” Cancer Research, 57:3635-3639, 1997
• Olumi AF, Grossfeld GD, Hayward SW, Carroll PR, Tlsty TD, Cunha GR “Carcinoma-associated Fiborblasts Direct Tumor Progression of Initiated Human Prostatic Epithelium” Cancer Research 61:5002-5011, 1999
• Romanov, S, Krystyna Kozakiewicz, Charles R. Holst, Martha R. Stampfer, Larisa M. Haupt, and Tlsty TD “Normal Human Mammary Epithelial Cells Spontaneously Emerge from Senescence and Acquire Genomic Instability” Nature, 409:633-637, 2001
• Holst CR, Nuovo GJ, Esteller M, Chew K, Baylin SB, Herman JG, Tlsty TD “Methylation of p16INK4a Promoters Occurs in Histologically Normal Human Mammary Epithelia” Cancer Research 63: 1596-1601, 2003.
• Crawford YG, Gauthier ML, Joubel A, Mantei K, Kozakiewicz K, Afshari CA, Tlsty TD. (2004) Histologically normal human mammary epithelia with silenced p16(INK4a) overexpress COX-2, promoting a premalignant program. Cancer Cell. 5(3):263-73.
• Reynolds PA, Sigaroudinia M, Zardo G, Wilson MB, Benton GM, Miller CJ, Hong C, Fridlyand J, Costello JF, Tlsty TD, “Tumor Suppressor p16INK4A Regulates Polycomb-Mediated DNA Hypermethylation in Human Mammary Epithelial Cells” J Biol Chem. J Biol Chem. Aug 25;281(34):24790-802, 2006
• Zhang J, Pickering CR, Holst CR, Gauthier ML, Tlsty TD. “p16INK4a Modulates p53 in Primary Human Mammary Epithelial Cells”, Cancer Res. Nov 1;66(21):10325-31, 2006
• Gauthier ML, Berman HK, Miller CJ, Kozakiewicz K, Chew K, Moore D, Rabban J, Chen YY, Kerlikowske K, Tlsty TD. “Abrogated response to cellular stress identifies DCIS associated with subsequent tumor events and defines basal-like breast tumors”, Cancer Cell 2007 Nov;12(5):479-91.
• Dumont N, Wilson MB, Crawford YG, Reynolds PA, Sigaroudinia M, Tlsty TD. “Sustained induction of epithelial to mesenchymal transition activates DNA methylation of genes silenced in basal-like breast cancers”. PNAS 2008, Sep 30;105(39):14867-72 PMID: 18806226
• Fordyce C, Fessenden T, Pickering C, Jung J, Singla V, Berman H, Tlsty T. “DNA damage drives an activin a-dependent induction of cyclooxygenase-2 in premalignant cells and lesions”, Cancer Prev Res (Phila Pa). 2010 Feb;3(2):190-201. Epub 2009 Dec 22
• Dumont N, Crawford YG, Sigaroudinia M, Nagrani SS, Wilson MB, Buehring GC, Turashvili G, Aparicio S, Gauthier ML, Fordyce CA, McDermott KM, Tlsty TD. Human mammary cancer progression model recapitulates methylation events associated with breast premalignancy. Breast Cancer Res. 2009;11(6):R87
• Chang H, DeFilippis RA, Tlsty TD, Parvin B. Graphical methods for quantifying macromolecules through bright field imaging. Bioinformatics. 2009 Apr 15;25(8):1070-5.
• Kerlikowske K, Molinaro AM, Gauthier ML, Berman HK, Waldman F, Bennington J, Sanchez H, Jimenez C, Stewart K, Chew K, Ljung BM, Tlsty TD. Biomarker expression and risk of subsequent tumors after initial ductal carcinoma in situ diagnosis. J Natl Cancer Inst. 2010 May 5;102(9):627-37.
• Berman HK, Gauthier ML, Tlsty TD. Premalignant breast neoplasia: a paradigm of interlesional and intralesional molecular heterogeneity and its biological and clinical ramifications. Cancer Prev Res (Phila Pa). 2010 May;3(5):579-87.
• McDermott KM, Liu BY, Tlsty TD, Pazour GJ. Primary Cilia Regulate Branching Morphogenesis during Mammary Gland Development. Curr Biol. 2010 Apr 7
• Blackburn EH, Tlsty TD, Lippman SM. Unprecedented opportunities and promise for cancer prevention research. Cancer Prev Res (Phila Pa). 2010 Apr;3(4):394-402.