delwarte@medicine.ucsf.edu
(415) 923-5763 (voice)
(415) 276-2311 (fax)

Blood Systems Research Institute, 270 Masonic Avenue, San Francisco, CA 94118

University of Newcastle, England, B.Sc., 1980, Biochemistry & Genetics
University of Geneva, Switzerland, M.Sc., 1982, Molecular Biology
University of Wisconsin at Madison, Ph.D., 1990, Oncology

M.E. Mirault, R. Southgate and E.L. Delwart. 1982. Regulation of heat-shock genes: a DNA sequence upstream of Drosophila hsp70 genes is essential for their induction in monkey cells. EMBO J. 1:1279-1285.

W.I. Wood, D.J. Capon, C.C. Simenson, D.L. Eaton, J. Gitschier, B. Keyt, P.H. Seeburg, D.H. Smith, P. Hollongshead, K.L. Wion, E.L. Delwart, E. Tuddenham, G.A. Vehar and R.M. Lawn. 1984. Expression of active human factor VIII from recombinant DNA clones. Nature 312:330-337.

D.G. Lowe, D.J. Capon, E.L. Delwart, A.Y. Sakaguchi, S.L. Naylor and D.V. Goeddel. 1987. Structure of the human and murine R-ras genes, novel genes closely related to ras proto-oncogenes. Cell 48:137-146.

E.L. Delwart and A.T. Panganiban. 1989. Role of reticuloendotheliosis virus envelope glycoprotein in superinfection interference. J. Virol. 63:273-280.

E.L. Delwart, G. Mosialos and T. Gilmore. 1990. Retroviral envelope glycoproteins contain a "leucine zipper"-like repeat. AIDS Res. Human Retroviruses 6:703-706.

E.L. Delwart and A.T. Panganiban. 1990. N-linked glycosylation and spleen necrosis retrovirus envelope protein function. Virology 179:648-657.

E.O. Freed, E.L. Delwart, G.L. Buchschacher Jr. and A.T. Panganiban. 1992. A mutation in the human immunodeficiency virus type 1 transmembrane glycoprotein gp41 dominantly interferes with fusion and infectivity. Proc. Natl. Acad. Sci. USA 89:70-75.

E.L. Delwart, G.L. Buchschacher Jr., E.O. Freed and A.T. Panganiban. 1992. Analysis of HIV-1 envelope mutants and pseudotyping of replication-defective vectors by genetic complementation. AIDS Res. Hum. Retroviruses 8:1669-1678.

Delwart, E.L., E.G. Shpaer, J. Louwagie, F.E. McCutchan, M. Grez, H. Rubsamen-Waigmann and J.I. Mullins. 1993. Evolutionary relationship between HIV env genes determined by a quantitative heteroduplex mobility assay. Science 262:1257-1261.

Delwart, E.L., H.W. Haynes, B.D. Walker, J. Goudsmit and J.I. Mullins. 1994. Human immunodeficiency virus type 1 evolution in vivo tracked by DNA heteroduplex assays. J. Virol. 68:6672-6683.

Sabino, E., E.L. Delwart, T.-H. Lee, A. Mayer, J.I. Mullins and M.P. Busch. 1994. Identification of low-level contamination of blood as basis for detection of HIV DNA in an anti-HIV negative specimen. J. AIDS 7:853-859.

Shpaer, E.G., E.L. Delwart, C.L. Kuiken, J. Goudsmit, M.H. Bachmann and J.I. Mullins. 1994. Conserved V3 loop sequences and the transmission of human immunodeficiency virus type 1. AIDS Res. Hum. Retroviruses 10:1679-1684.

M.L. Kalish, A. Baldwin, S. Raktham, C. Wasi, C.-C. Luo, G. Schochetman, T.D. Mastro, N.L. Young, S. Vanichseni, H. Rubsamen-Waigman, H. von Briesen, J.I. Mullins, E.L. Delwart, B. Herring,, J. Esparza, W.L. Heyward and S. Osmanov. 1995. The evolving molecular epidemiology of HIV-1 envelope in injecting drug users in Bangkok, Thailand: implications for HIV vaccine trials. AIDS 9:851-857.

WHO Network for HIV Isolation and Characterization. 1994. HIV type 1 variation in World Health Organization-sponsored vaccine evaluation sites: Genetic screening, sequence analysis, and preliminary biological characterization of selected viral strains. AIDS Research and Human Retroviruses 10:1327-1343.

Delwart, E.L., M.P. Busch, M.L. Kalish, J.W. Mosley and J.I. Mullins. 1995. Rapid molecular epidemiology of HIV-1 transmission. AIDS Res. Hum. Retroviruses 11:1181-1193.

Delwart, E.L. and C.J. Gordon. 1997. Tracking changes in HIV-1 envelope quasispecies using DNA heteroduplex analysis. METHODS: A companion to methods in enzymology 12: 348-354.

Delwart, E.L., H. Pan, H.W. Sheppard, D. Wolpert, A.U. Neumann, B. Korber, J.I. Mullins. 1997. Slower HIV-1 evolution during progression to AIDS. J. Virol. 71:7498-7508.

Delwart, E.L., J.I. Mullins, P. Gupta, G.H. Learn Jr., M. Holodniy, D. Katzenstein, B.D. Walker, M.K. Singh. 1997. Human immunodeficiency virus type 1 populations in blood and semen. J. Virol. 72:617-623.

Delwart, E.L., H. Pan, A. Neumann, M. Markowitz. 1998. Rapid, transient, changes at the env locus of plasma HIV-1 populations during the emergence of protease inhibitor resistance. J. Virol. 72:2416-2421.

Shen, D.-F., L. Doukhan, S. Kalams, and E.L. Delwart. 1998. High resolution analysis of T-cell receptor beta chain repertoires using DNA heteroduplex tracking: major, generally stable, clonal CD8+ cells expansions in healthy young adults. J. Immunol. Meth.215: 113-21.

Rodrigo, A.G., E.G. Shpear, E.L. Delwart, A.K.N. Iversen, M.V. Gallo, J. Brojatsch, M.S. Hirsh, B.D. Walker and J.I. Mullins. 1999. Coalescent estimates of HIV-1 generation time in vivo. Proc.Natl. Acad. Sci. USA 96: 2187-91.

Foley B., H. Pan, S. Buchbinder and E.L. Delwart. 2000. Apparent founder effect during the early HIV-1 epidemic in San Francisco (1978-79). AIDS Res. Hum. Retroviruses 16:1463-1469.

J. Gordon and E.L. Delwart. 2000. Genetic diversity of primary HIV-1 isolates and their sensitivity to antibody-mediated neutralization. Virology 272:326-330.

L. Doukhan and E.L. Delwart. 2001. Population genetic analysis of the protease locus of HIV-1 quasispecies undergoing drug selection using a denaturing gradient-heteroduplex tracking assay. J. Virol.75:6729-6736.

D. Machado, E.L. Delwart, C. F. Deoliveira, R. S. Diaz, B. D. Rawal, M. Sullivan, M. Gwinn, and M. Busch. 2002. Genetic analysis of HIV-1 in recently infected US blood donors identified using the dual sensitive/less sensitive EIA strategy. AIDS. 16:113-119.

E.L. Delwart, M. Magierowska, M. Royz, B. Foley, L. Peddada, R. Smith, C Heldebrand, A Conrad, M.P. Busch. 2002. Homogeneous quasispecies in 16/17 individuals during very early HIV-1 primary infection. AIDS 16:189-195.

K. Alves, M. Canzian and E.L. Delwart. HIV-1 Envelope Quasispecies in the Thymus and Lymph Nodes of AIDS Patients. 2002. AIDS Res. Hum. Retroviruses 18:161-165.

F. Bernardin, L. Doukhan, A. Longone-Miller, P. Champagne, R. Sekaly, and E. L. Delwart. Analysis of the complete TRBV CDR3 repertoire using a DNA heteroduplex-tracking assay: the majority of CD8+ subfamilies are oligoclonal. 2003. J. Immunol. Methods. 274:159-175.

M. Gonzales, E. Delwart, S.-Y. Rhee, R. Tsui, J. Taylor, R. W. Shafer. 2003.Lack of detectable superinfection during 1072 person years of observation. J. Infectious Diseases 188:397-405.

E. Delwart, S. Orton, B. Parekh, T. Dobbs, K. Clark, M. P. Busch. 2003. 2% of HIV positive US blood donors are infected with non-subtype B strains. AIDS. AIDS Res. Hum. Retroviruses 19:1065-1070

F. Bernardin, B. L. Herring, L. Peddada and E. Delwart. 2003. Primary infection of a male plasma donor with two highly divergent HIV variants from the same source followed by rapid fluctuations in their relative frequency and viral recombination. AIDS Res. Hum. Retroviruses 19:1009-1015

R. Tsui, B. Herring, J. D. Barbour, R. M. Grant, P. Bacchetti, A. Kral, B. Edlin, E. Delwart. 2004. HIV1 superinfection was not detected following 215 years of injection drug user exposure. J.Virology. 78:94-103.

M. Magierowska, F. Bernardin, S. Garg, S. Silvija, M. Miller, K. Van Rompay, E. Delwart. 2004. Highly uneven distribution of tenofovir-selected SIV in different anatomical sites of rhesus macaques. J.Virology. 78: 2434-2444.

J. Angel, Y.-W. Hu, S. Kravcik, R. Tsui, K. H. Lee, J. Barbour, E, Balaskas, B. Branson, E. Delwart, R. M. Grant. 2004. Virological evaluation of the "Ottowa case" reveals no evidence for HIV superinfection. AIDS.. 18:331-333.

E. Delwart, N.D. Kalmin, T.S. Jones, D. J. Ladd, L. H. Tobler, R. Tsui, M.P.. Busch. 2004. First Case of HIV Transmission by an RNA Screened Blood Donation. Vox Sanguinis. 86:171-177.

F. Bernardin, M. Magierowska, S. Dandekar, K. A. Van Rompay, E. Delwart. 2004. Number of CD4+ and CD8+ T-cell CDR3 clonotypes expanding during acute infection of macaques with SIV. Virology.322:105-117.

Kapoor, M. S. Jones, B. Shafer, S.-Y. Rhee, P. Kazanjian, E. Delwart. 2004. Enhanced detection of low-frequency HIV-1 drug resistant mutants using an RNA/DNA heteroduplex generator-tracking assay. J. Virology. 78:7112-7123.

B. Herring, K. Page-Schafer, L. Tobler, E. Delwart. No protection against HCV superinfection in injection drug users. J. Infectious Diseases. 2004. J. Infectious Diseases 190:1396-1403.

New DNA viruses identified in patients with acute viral infection syndrome. 2004. M. S. Jones, A. Kapoor, V. V. Lukashov, P. Simmonds, F. Hecht, E. Delwart. J. of Virology. In press.

Wide range of quasispecies diversity during primary HCV infection. 2004. B. L. Herring, R. Tsui, L. Peddada, M. Busch, E. Delwart. J. of Virology. In press.

1/15/05