Jengel@medicine.ucsf.edu
(415) 476-7355 (voice)
(415) 476-9364 (fax)

Box 0654, UCSF; San Francisco, CA 94143-0654

additional websites:

Engel Lab Website

Yale University, New Haven, CT, B.S., 1976, Biochemistry
Stanford University, Palo Alto, CA, Ph.D., 1982, Biophysics
Stanford University, Palo Alto, CA, M.D., 1983, Medicine

(out of 53)

Tan, M., Klein, R., Grant, R., Ganem, D. and Engel, J. N. Cloning and Characterization of the RNA polymerase a subunit operon of Chlamydia trachomatis, Journal of Bacteriology, 175:7150-7159, 1993.

Dell, K. and Engel, J. N., Stage specific regulation of protein phosphorylation in Leishmania major, Molecular and Biochemical Parasitology, 64:283-292, 1994.

Apodaca, G., Bomsel, M., Lindstedt, R., Engel, J. Frank, D., Mostov, K. E., and Wiener-Kronish, J. Characterization of Pseudomonas aeruginosa-induced MDCK cell Injury: Glycosylation defective host cells are resistant to bacterial killing, Infection and Immunity, 63:1541-1551, 1995.

Tan, M., and Engel, J. N., Identification of sequences necessary for transcription in vitro of the Chlamydia trachomatis rRNA P1 promoter, Journal of Bacteriology. 178: 6975-6982, 1996

Tan, M., Wong, B., and Engel, J. N. The transcriptional organization and regulation of the dnaK and groE Operons of Chlamydia trachomatis, Journal of Bacteriology, 178: 6983-6990, 1996

van Ooij, C., Apodoca, G., and Engel, J. N., Characterization of the Chlamydia trachomatis vacuole and its interaction with the host endocytic pathway in HeLa cells, Infection and Immunity, 65:758-766, 1997.

Kang, P. J., Hauser, A., Apodaca, G., Wiener-Kronish, J., Mostov, K., Fleizig, S., and Engel, J., Identification of Pseudomonas aeruginosa Genes required for epithelial cell Injury, Mol. Microbiology, 24:1249-1262, 1997.

Fawaz, F., Van Ooij, C., Homola, E., Mutka, S., and Engel, J. Infection with Chlamydia trachomatis alters the tyrosine phosphorylation and/or localization of several host cell proteins including cortactin, Infection and Immunity, 65: 5301-5308, 1997.

Hauser, A., Kang, P., and Engel, J. PepA, a novel secreted protein of Pseudomonas aeruginosa, is necessary for cytotoxicity and virulence, Molecular Microbiology, 27:807-818,1998.

Hauser, A., Fleizsig, S., Kang, P. J., Mostov, K., and Engel, J., Defects in type III secretion correlate with internalization of Pseudomonas aeruginosa by epithelial cells, Infection & Immunity, 66:1413-1420, 1998.

Tan, M., Gaal, Tamas, Gourse, R., and Engel, J. Point substitution analysis of the rRNA P1 promoter of Chlamydia trachomatis defines four regions important for transcription in vitro, Journal of Bacteriology, 180:2359-2366, 1998.

van Ooij, C., Homola, E., Kincaid, E., and Engel, J. Fusion of vacuoles containing Chlamydia trachomatis is inhibited at low temperature and requires bacterial protein synthesis, Infection and Immunity, 66:5364-5371,1998.

Comolli, J., Waite, L., Mostov, K., and Engel, J. Pili binding to asialo-GM1 on epithelial cells can mediate cytotoxicity or bacterial internalization by Pseudomonas aeruginosa, Infection and Immunity, 67: 3207-3214, 1999

Comolli, J., Hauser, A., Waite, L., Whitchurch, C., Mattick, J., and Engel, J. Pseudomonas aeruginosa gene products PilT and PilU are required for cytotoxicity in vitro and virulence in a mouse model of acute pneumonia, Infection and Immunity, 67:3625-3630, 1999

Hauser, A., and Engel, J., Pseudomonas aeruginosa induces Type III secretion-mediated apoptosis, Infection and Immunity, 67: 5530-5537, 1999.

Stephens, R. S., Fawaz, F. S., Kennedy, K. A., Koshiyama, K., Nichols, B., van Ooij, C., and Engel, J. N.Eukaryotic Cell Uptake of Heparin-Coated Microspheres: A Model of Host Cell Invasion by Chlamydia, Infection and Immunity, 68:1080-1085, 2000.

van Ooij, C., Kalman, L., van IJzendoorn, S., Nishijima, M., Hanada, K., Mostov, K., and Engel, J. Host-cell derived sphingolipids are required for the intracellular growth of Chlamydia trachomatis, Cellular Microbiology, 2:627-638, 2000.

Garrity-Ryan, L, Kazmierczak, B., Kowal, R., Comolli, J., Hauser, A. and Engel, J. The arginine finger domain of ExoT contributes to actin cytoskeleton disruption and inhibition of internalization of Pseudomonas aeruginosa into epithelial cells and macrophages, Infection and Immunity, 68: 7100-7113, 2000.

Kazmierczak, B., Jou, T.-S., Mostov, K., and Engel, J. Rho-family GTPases Modulate Pseudomonas aeruginosa Internalization by Epithelial Cells, Cellular Microbiology, 3:85-98, 2001.

Geiser, T., Kazmierczak, B., Garrity-Ryan, L, Matthay, M., and Engel, J., Pseudomonas aeruginosa ExoT inhibits in vitro lung epithelial wound repair, Cellular Microbiology, 3:223-236, 2001.

Kazmierczak, B., Mostov, K., and Engel, J. Interaction of bacterial pathogens with polarized epithelium, Annual Review of Microbiology, 55:407-435, 2001.

Hauser, A. R., E. Cobb, M. Bodi, D. Mariscal, J. Valles, J. N. Engel, and J. Rello. Type III protein secretion is associated with poor clinical outcomes in patients with ventilator-associated pneumonia caused by Pseudomonas aeruginosa. Crit. Care Med. 30:521-528, 2002.

Kazmierczak, B. and Engel, J., Pseudomonas aeruginosa ExoT acts in vivo as a GTPase activating protein for RhoA, Rac1, and Cdc42, Infection and Immunity, 74:2198, 2002.

Engel, J. Molecular Pathogenesis of acute Pseudomonas aeruginosa infections. In Severe Infections Caused by Pseudomonas aeruginosa, A. Hauser and J. Rello, editors, Kluwer/Plenum Press, New York City, 2002.

Jacob, T, Lee, R., Engel, J., and Machen, T. The effect of apical vs basolateral Pseudomonas Aeruginosa on Ca signaling in airway epithelial cells, Infection and Immunity, 70:6399-6408, 2002.

McMorran B., Town,L., Costello, E., Palmer, J., Engel, J., Hume, D., and Wainright, B.The type III¨¢secreted effector ExoU is an important modulator of gene expression in lung epithelial cells in response to Pseudomonas aeruginosa infection, Infection and Immunity, 71:6035-6044, 2003.

Garrity-Ryan, L, Shafikhani, S., Balachandran, P., Nguyen, L, Oza, J., Jakobsen, T., Sargent, J., Fang, X., Matthay, M.. and Engel, J. The ADP ribosyltranserase domain of Pseudomonas aeruginosa ExoT contributes to its biological activities, Infection and Immunity, 72:546-558, 2004.

Kazmierczak, B., Jou, T.-S., Mostov, K., and Engel, J., Epithelial cell polarity alters Rho-GTPase responses to Pseudomonas aeruginosa, Molecular Biology of the Cell, 15:411-419, 2004.

Whitchurch CB, Leech AJ, Young MD, Kennedy D, Sargent JL, Bertrand JJ, Semmler AB, Mellick AS, Martin PR, Alm RA, Hobbs M, Beatson SA, Huang B, Nguyen L, Commolli JC, Engel JN, Darzins A, Mattick JS,Characterisation of a complex signal transduction system which controls twitching motility and the production of multiple virulence factors of Pseudomonas aeruginosa, Molecular Microbiology, 52:873-93, 2004.

Gao L.Y., Guo S., Morisaki H., Horwitz M.A., Engel J.N., and Brown E.J. A mycobacterial gene cluster involved in virulence is required for cytotoxicity, bacterial cell-to-cell spread, and ESAT-6 secretion, Molecular Microbiology, 53:1677-93, 2004

Gabel, B., van IJzendoorn, S., Engel, J. Lipid-raft mediated entry is not required for Chlamydia trachomatis infection of cultured epithelial cells, Infection and Immunity, 72:7367-73, 2004.

Engel, J. Tarp and Arp: How Chlamydia induces its own entry, Proceedings of the National Academy of Sciences, 101: 9947-8, 2004.

Whitchurch, C., Beatson, S., Comolli, Sargent, J., Bertrand, J. , West, J., Klausen, M., Waite, L., Kang, P. J., Tolker-Nielson, T., Mattick, J., and Engel, J. FimL, a novel Pseudomonas aeruginosa gene product involved in twitching motility, in press, Molecular Microbiology, 2005.

Elwell, C. and Engel, J. Chlamydia trachomatis infection of Drosophila melanogaster S2 cells mimics early steps in mammalian infection, in press, Cellular Microbiology, 2005.

Kierbel, A., Gassama, A., Mostov, K., and Engel, J., The Phosphoinositol-3-kinase-Protein kinase B/Akt pathway is critical for Pseudomonas aeruginosa strain PAK internalization, in press, Molecular Biology of the Cell.

Agron, P., Reed, S., and Engel, J. An Essential, Putative MEK Kinase of Leishmania major, in press, Molecular and Biochemical Parasitology.

5/01/05