Joanne Netter Engel, MD, PhD

Yale University, New Haven, CT, B.S., 1976, Biochemistry
Stanford University, Palo Alto, CA, Ph.D., 1982, Biophysics
Stanford University, Palo Alto, CA, M.D., 1983, Medicine

• June 1983 - June 1984
  Hospital of the University of Pennsylvania,Internship, Department of Medicine
• June 1984 - Jan 1986
  Hospital of the University of Pennsylvania, Residency, Department of Medicine
• Jan 1986 - July 1986
  Residency, Department of Medicine, UCSF
• July 1986 - June 1990
  Clinical and Postdoctoral Fellowship, Div. of Infectious Diseases, Department of Microbiology and Immunology, UCSF
• July 1990 - June 1998
  Assistant Professor in Residence, Div. of Infectious Diseases, Department of Medicine, UCSF
• July 1998 - June 2004
  Associate Professor, Division of Infectious Disease and Department of Microbiology and Immunology
• July 2004 - present
  Professor in Residence, University of California, San Francisco, Depts. of Medicine and Microbiology and Immunology
• July 2004 - present
  Director, Microbial Pathogenesis and Host Defense Program
• January 2005 - present
  Interim Chief, Division of Infectious Disease

• 1976
  Phi Beta Kappa
• 1976
  Sigma Xi
• 1976
  Magna cum laude, Yale University
• 1977
  Medical Scientist Training Program
• 1988
  Lucille Markey Biomedical Scholar
• 1994
  NIH Career Development Award
• 1998
  American Lung Association Career Investigator
• 2004
  President-Elect, Division B of American Society for Microbiology

• (out of 53)
• Tan, M., Klein, R., Grant, R., Ganem, D. and Engel, J. N. Cloning and Characterization of the RNA polymerase a subunit operon of Chlamydia trachomatis, Journal of Bacteriology, 175:7150-7159, 1993.
• Dell, K. and Engel, J. N., Stage specific regulation of protein phosphorylation in Leishmania major, Molecular and Biochemical Parasitology, 64:283-292, 1994.
• Apodaca, G., Bomsel, M., Lindstedt, R., Engel, J. Frank, D., Mostov, K. E., and Wiener-Kronish, J. Characterization of Pseudomonas aeruginosa-induced MDCK cell Injury: Glycosylation defective host cells are resistant to bacterial killing, Infection and Immunity, 63:1541-1551, 1995.
• Tan, M., and Engel, J. N., Identification of sequences necessary for transcription in vitro of the Chlamydia trachomatis rRNA P1 promoter, Journal of Bacteriology. 178: 6975-6982, 1996
• Tan, M., Wong, B., and Engel, J. N. The transcriptional organization and regulation of the dnaK and groE Operons of Chlamydia trachomatis, Journal of Bacteriology, 178: 6983-6990, 1996
• van Ooij, C., Apodoca, G., and Engel, J. N., Characterization of the Chlamydia trachomatis vacuole and its interaction with the host endocytic pathway in HeLa cells, Infection and Immunity, 65:758-766, 1997.
• Kang, P. J., Hauser, A., Apodaca, G., Wiener-Kronish, J., Mostov, K., Fleizig, S., and Engel, J., Identification of Pseudomonas aeruginosa Genes required for epithelial cell Injury, Mol. Microbiology, 24:1249-1262, 1997.
• Fawaz, F., Van Ooij, C., Homola, E., Mutka, S., and Engel, J. Infection with Chlamydia trachomatis alters the tyrosine phosphorylation and/or localization of several host cell proteins including cortactin, Infection and Immunity, 65: 5301-5308, 1997.
• Hauser, A., Kang, P., and Engel, J. PepA, a novel secreted protein of Pseudomonas aeruginosa, is necessary for cytotoxicity and virulence, Molecular Microbiology, 27:807-818,1998.
• Hauser, A., Fleizsig, S., Kang, P. J., Mostov, K., and Engel, J., Defects in type III secretion correlate with internalization of Pseudomonas aeruginosa by epithelial cells, Infection & Immunity, 66:1413-1420, 1998.
• Tan, M., Gaal, Tamas, Gourse, R., and Engel, J. Point substitution analysis of the rRNA P1 promoter of Chlamydia trachomatis defines four regions important for transcription in vitro, Journal of Bacteriology, 180:2359-2366, 1998.
• van Ooij, C., Homola, E., Kincaid, E., and Engel, J. Fusion of vacuoles containing Chlamydia trachomatis is inhibited at low temperature and requires bacterial protein synthesis, Infection and Immunity, 66:5364-5371,1998.
• Comolli, J., Waite, L., Mostov, K., and Engel, J. Pili binding to asialo-GM1 on epithelial cells can mediate cytotoxicity or bacterial internalization by Pseudomonas aeruginosa, Infection and Immunity, 67: 3207-3214, 1999
• Comolli, J., Hauser, A., Waite, L., Whitchurch, C., Mattick, J., and Engel, J. Pseudomonas aeruginosa gene products PilT and PilU are required for cytotoxicity in vitro and virulence in a mouse model of acute pneumonia, Infection and Immunity, 67:3625-3630, 1999
• Hauser, A., and Engel, J., Pseudomonas aeruginosa induces Type III secretion-mediated apoptosis, Infection and Immunity, 67: 5530-5537, 1999.
• Stephens, R. S., Fawaz, F. S., Kennedy, K. A., Koshiyama, K., Nichols, B., van Ooij, C., and Engel, J. N.Eukaryotic Cell Uptake of Heparin-Coated Microspheres: A Model of Host Cell Invasion by Chlamydia, Infection and Immunity, 68:1080-1085, 2000.
• van Ooij, C., Kalman, L., van IJzendoorn, S., Nishijima, M., Hanada, K., Mostov, K., and Engel, J. Host-cell derived sphingolipids are required for the intracellular growth of Chlamydia trachomatis, Cellular Microbiology, 2:627-638, 2000.
• Garrity-Ryan, L, Kazmierczak, B., Kowal, R., Comolli, J., Hauser, A. and Engel, J. The arginine finger domain of ExoT contributes to actin cytoskeleton disruption and inhibition of internalization of Pseudomonas aeruginosa into epithelial cells and macrophages, Infection and Immunity, 68: 7100-7113, 2000.
• Kazmierczak, B., Jou, T.-S., Mostov, K., and Engel, J. Rho-family GTPases Modulate Pseudomonas aeruginosa Internalization by Epithelial Cells, Cellular Microbiology, 3:85-98, 2001.
• Geiser, T., Kazmierczak, B., Garrity-Ryan, L, Matthay, M., and Engel, J., Pseudomonas aeruginosa ExoT inhibits in vitro lung epithelial wound repair, Cellular Microbiology, 3:223-236, 2001.
• Kazmierczak, B., Mostov, K., and Engel, J. Interaction of bacterial pathogens with polarized epithelium, Annual Review of Microbiology, 55:407-435, 2001.
• Hauser, A. R., E. Cobb, M. Bodi, D. Mariscal, J. Valles, J. N. Engel, and J. Rello. Type III protein secretion is associated with poor clinical outcomes in patients with ventilator-associated pneumonia caused by Pseudomonas aeruginosa. Crit. Care Med. 30:521-528, 2002.
• Kazmierczak, B. and Engel, J., Pseudomonas aeruginosa ExoT acts in vivo as a GTPase activating protein for RhoA, Rac1, and Cdc42, Infection and Immunity, 74:2198, 2002.
• Engel, J. Molecular Pathogenesis of acute Pseudomonas aeruginosa infections. In Severe Infections Caused by Pseudomonas aeruginosa, A. Hauser and J. Rello, editors, Kluwer/Plenum Press, New York City, 2002.
• Jacob, T, Lee, R., Engel, J., and Machen, T. The effect of apical vs basolateral Pseudomonas Aeruginosa on Ca signaling in airway epithelial cells, Infection and Immunity, 70:6399-6408, 2002.
• McMorran B., Town,L., Costello, E., Palmer, J., Engel, J., Hume, D., and Wainright, B.The type IIIsecreted effector ExoU is an important modulator of gene expression in lung epithelial cells in response to Pseudomonas aeruginosa infection, Infection and Immunity, 71:6035-6044, 2003.
• Garrity-Ryan, L, Shafikhani, S., Balachandran, P., Nguyen, L, Oza, J., Jakobsen, T., Sargent, J., Fang, X., Matthay, M.. and Engel, J. The ADP ribosyltranserase domain of Pseudomonas aeruginosa ExoT contributes to its biological activities, Infection and Immunity, 72:546-558, 2004.
• Kazmierczak, B., Jou, T.-S., Mostov, K., and Engel, J., Epithelial cell polarity alters Rho-GTPase responses to Pseudomonas aeruginosa, Molecular Biology of the Cell, 15:411-419, 2004.
• Whitchurch CB, Leech AJ, Young MD, Kennedy D, Sargent JL, Bertrand JJ, Semmler AB, Mellick AS, Martin PR, Alm RA, Hobbs M, Beatson SA, Huang B, Nguyen L, Commolli JC, Engel JN, Darzins A, Mattick JS,Characterisation of a complex signal transduction system which controls twitching motility and the production of multiple virulence factors of Pseudomonas aeruginosa, Molecular Microbiology, 52:873-93, 2004.
• Gao L.Y., Guo S., Morisaki H., Horwitz M.A., Engel J.N., and Brown E.J. A mycobacterial gene cluster involved in virulence is required for cytotoxicity, bacterial cell-to-cell spread, and ESAT-6 secretion, Molecular Microbiology, 53:1677-93, 2004
• Gabel, B., van IJzendoorn, S., Engel, J. Lipid-raft mediated entry is not required for Chlamydia trachomatis infection of cultured epithelial cells, Infection and Immunity, 72:7367-73, 2004.
• Engel, J. Tarp and Arp: How Chlamydia induces its own entry, Proceedings of the National Academy of Sciences, 101: 9947-8, 2004.