Patrick H. O’Farrell, PhD

McGill University, Montreal, Quebec, B.Sc., 1969, Genetics
University of Colorado, Boulder, Ph.D., 1974, Cell & Dev Biol
University of California San Francisco, Postdoc, 1978

• 1969-1974
  Grad. Student: lab of Jacques Pene (1969-72) & David Hirsh (1973 -74). Development of 2-D gel electrophoresis; pleiotropic control. MCD Biology, University of Colorado
• 1974-1976
  Postdoctoral studies with Gordon Tomkins. Regulation of translational accuracy; hormonal control; expression of recombinant DNA. Dept of Biochemistry, UCSF
• 1976-1979
  Postdoctoral studies with Bruce Alberts. Stringency of gene inactivation; new preparative separation technology. Dept of Biochemistry, UCSF
• 1979-1985
  Assistant Professor, Department of Biochemistry & Biophysics, UCSF
• 1985-1989
  Associate Professor, Department of Biochemistry & Biophysics, UCSF
• 1986
  Sabbatical research, with Dr. Christiane Nüsslein-Volhard, Tubingen, FRG
• 1989-present
  Professor, Department of Biochemistry & Biophysics, UCSF
• 1995-1996
  Sabbatical research with Dr. Gerald Rubin, University of California, Berkeley

• Fellow of the Jane Coffin Childs Memorial Fund for Medical Research
• Senior Fellow of the California Division of the American Cancer Society
• Award - Sarstedt Research Prize for the development of electrophoretic separations
• Chairman of the Biological Regulatory Mechanism Gordon Conference
• Member NSF Developmental Biology Review Panel
• Chairman of the Molecular Genetics Gordon Conference
• Scientific Review Board, Howard Hughes Institute
• Advisory Panel to the NCI

• O'Farrell PH (1975) High resolution two-dimensional electrophoresis of proteins. J. Biol. Chem. 250: 4007.
• O'Farrell PH (1978) The suppression of defective translation by ppGpp and its role in the stringent response. Cell 14: 545-557.
• O'Farrell PH (1985). Separation techniques based on the opposition of two counteracting forces to produce a dynamic equilibrium. Science 227: 1586-1589.
• Desplan C, Theis J, and O'Farrell PH (1985) The Drosophila developmental gene, engrailed, encodes a sequence-specific DNA binding activity. Nature (Article) 318: 630-635.
• DiNardo S, Kuner JM, Theis J and O'Farrell PH (1985) Development of embryonic pattern in D. melanogaster as revealed by accumulation of the nuclear engrailed protein. Cell 43: 59-69.
• DiNardo S, Sher E, Heemskerk-Jongens J, Kassis JA and O'Farrell PH (1988) Two-tiered regulation of spatially patterned en expression during Drosophila embryogenesis. Nature (Article) 332: 604-609.
• Desplan, C., Theis, J., and O'Farrell, P.H. (1988) The sequence specificity of homeodomain-DNA interaction. Cell 54: 1081-1090.
• Jaynes, J.B. and O'Farrell, P.H. (1988) Activation and repression of transcription by homeodomain containing proteins that bind a common site. Nature (Article) 336: 744-749.
• Lehner, C.F., and O'Farrell, P.H. (1989) Expression and function of Drosophila cyclin A during embryonic cell cycle progression. Cell, 56: 957-968.
• Edgar, B.A., and O'Farrell, P.H. (1989) Genetic control of cell division patterns in the Drosophila embryo. Cell 57: 177-187.
• O'Farrell, P.H., Edgar, B.A., Lakich, D. and Lehner, C.F. (1989) Directing cell division during development. Science 246: 635-640.
• Lehner, C.F. and O'Farrell, P.H. (1990) The roles of cyclins A and B in mitotic control. Cell 61: 535-547.
• Edgar, B.A. and O'Farrell, P.H. (1990) The three post-blastoderm cell cycles of Drosophila embryogenesis are regulated in G2 by string. Cell 62: 469-480.
• Heemskerk, J., DiNardo, S., Kostriken, R. and O'Farrell, P.H. (1991) Multiple modes of engrailed regulation in the progression toward cell fate determination. Nature (Article) 352:404-410
• Shermoen, A. W. and O'Farrell, P.H. (1991) Progression of the cell cycle through mitosis causes abortion of nascent transcripts Cell 67: 303-310
• Leopold, P. and O'Farrell, P.H. (1991) An evolutionarily conserved cyclin homolog from Drosophila rescues yeast deficient in G1 cyclins. Cell 66: 1207-1216
• Vincent, J-P., and O'Farrell P.H. (1992) The state of engrailed expression is not clonally transmitted during early Drosophila development. Cell 68: 923-931
• Edgar BA, Sprenger F, Duronio RJ, Léopold P and O'Farrell PH (1994) Distinct molecular mechanisms regulate the cell cycle at successive stages of Drosophila embryogenesis. Genes and Dev. 8, 440-451
• Duronio RJ. and O'Farrell, PH. (1995) Developmental control of the G1 to S transition in Drosophila: cyclin E is a limiting downstream target of E2F. Genes and Dev. 9, 1456-1468
• Su TT, Follette PJ, and O'Farrell PH (1995) Qualifying for the license to replicate. Cell 81, 825-828
• Su, T. T., and O'Farrell, P. H. (1998) Chromosome association of MCM proteins in Drosophila endoreplication cycles. J. Cell Biol. 140: 451-460
• Follette, P. J., Duronio, R. J., and O'Farrell, P. H. (1998) Fluctuations in cyclin E levels are required for multiple rounds of endocycle S phase in Drosophila. Current Biol.8: 235-238
• Su, T. T., Sprenger, F., DiGregorio, P., Campbell, S. D. and O'Farrell, P. H. (1998) Exit from mitosis in Drosophila syncytial embryos requires proteolysis and cyclin degradation, and is associated with localized dephosphorylation. Genes and Dev. 12: 1495-1503
• Wingrove, J. A. and O'Farrell, P. H. (1999) Nitric oxide mediates behavioral, cell cycle and developmental responses to low oxygen in Drosophila. Cell. 98, 105-114
• Vidwans, S, Wong, M. L., Sprenger, M. and O'Farrell, P. H. (1999) Coupling between the centrosome cycle and the cell cycle during Drosophila embryogenesis. J. Cell Biol. 147: 1371-1378
• Rubin GM, et al., ( 2000) Comparative genomics of the eukaryotes. Science. 287: 2204-15.
• DiGregorio PJ, Ubersax JA, and O'Farrell PH. (2001) Hypoxia and nitric oxide induce a rapid, reversible cell cycle arrest of the Drosophila syncytial divisions J Biol Chem. 276: 1930-1937
• Parry, DH, and O'Farrell PH. (2001) The schedule of destruction of three mitotic cyclins can dictate the timing of events during exit from mitosis. Current Biology 11: 671-683
• O'Farrell, PH (2001) Triggering the all-or-nothing switch into mitosis. Trends Cell Biol. 11:512-9
• Vidwans, SJ., DiGregorio, PJ., Shermoen, AW., Foat, B., Iwasa, J., Yakubovitch, N., and O'Farrell, PH. (2002) Sister chromatids fail to separate during an induced endoreplication cycle in Drosophila embryos. Curr Biol 12:829-3390.
• Vidwans, SJ., Wong, ML., and O'Farrell, PH. (2003) Anomalous centriole configurations provide insights into the process of centriole duplication. J Cell Sci 116:137-143 Faculty 1000 choice
• Foley, E., and O'Farrell, PH. (2003) Nitric oxide contributes to induction of innate immune responses to gram negative bacteria in Drosophila . Genes and Dev. 17: 115-125- commentary: Dev Cell 4:5/Faculty 1000 choice
• Teodoro, RO and O'Farrell, PH (2003) Nitric oxide-induced suspended animation promotes survival during hypoxia EMBO J. 22, 580 -587 - Editors choice SCIENCE
• Echard, A and O'Farrell, PH (2003) The degradation of two mitotic cyclins contributes to the timing of cytokinesis. Curr. Biol. 13:373-83
• Parry, D., Hickson GRX and O'Farrell, PH (2003) Cyclin B destruction triggers changes in kinetochore behavior essential for successful anaphase. Curr. Biol. 13:647-653 Faculty 1000 choice
• Hériché, JK., Ang, D., Bier, E., and O'Farrell, PH. (2003) Involvement of an SCFSlmb complex in timely elimination of E2F upon initiation of DNA replication in Drosophila. BMC Genet.4:9
• O'Farrell PH (2003) How metazoans reach their full size: The natural history of bigness. Chapter in "Cell Growth" Ed. Michael Hall, Martin Raff, and George Thomas, Cold Spring Harbor Press, CSH NY
• O'Farrell PH, Stumpff J and Su TT (2004) Embryonic cleavage cycles: How is a mouse like a fly? Current Biol. 14: R35-45