David A. Agard, PhD

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Additional Websites

• Agard Lab Website

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Education

Yale University, New Haven, B.S., 1975, Molec. Biol. and Biophysics
California Institute of Technology, Pasadena, Ph.D., 1980, Biological Chemistry
University of California, San Francisco, Postdoctoral, 1980
MRC Laboratory, Cambridge, England, Postdoctoral, 1981-82

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Professional Experience

• 1975-1978
  National Science Foundation Predoctoral Fellow, California Institute of Technology.
• 1980
  Postdoctoral Fellow with Dr. J.W. Sedat, Dept. of Biochemistry and Biophysics University of California, San Francisco (UCSF)
• 1981
  Postdoctoral Fellow with Dr. R. Henderson, MRC Laboratory of Molecular Biology, Cambridge, England
• 1983-1989
  Assistant Professor of Biochemistry & Biophysics, UCSF
• 1986-1989
  Assistant Investigator in Structural Biology, Howard Hughes Medical Institute, UCSF
• 1989-1992
  Associate Professor of Biochemistry & Biophysics, UCSF
• 1986-1992
  Associate Investigator in Structural Biology, Howard Hughes Medical Institute, UCSF
• 1992-present
  Professor of Biochemistry & Biophysics and of Pharmaceutical Chemistry, UCSF
• 1993-present
  Investigator in Structural Biology, Howard Hughes Medical Institute UCSF
• 1995-present
  Chair, Graduate Group in Biophysics, UCSF
• 2001-2002
  Director, Institute for Bioengineering, Biotechnology, and Quantitative Biomedical Research, University of California, San Francisco, Berkeley, Santa Cruz
• 2002-present
  Scientific Director, Institute for Bioengineering, Biotechnology, and Quantitative Biomedical Research, University of California, San Francisco, Berkeley, Santa Cruz

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Honors & Awards

• 1975-1978
  National Science Foundation Predoctoral Fellow
• 1976
  Dreyfus Travelling Fellowship
• 1980-1982
  Helen Hay Whitney Postdoctoral Fellow
• 1983-1986
  Searle Scholar
• 1983-1991
  Presidential Young Investigator's Award (NSF)
• 1986
  Sidhu Award for Outstanding Contributions to Crystallography

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Selected Publications

1. Genest O, Reidy M, Street TO, Hoskins JR, Camberg JL, Agard DA, Masison DC, Wickner S. Uncovering a Region of Heat Shock Protein 90 Important for Client Binding in E. coli and Chaperone Function in Yeast. Mol Cell. 2013 Feb 7; 49(3):464-73.
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2. Abrahamsson S, Chen J, Hajj B, Stallinga S, Katsov AY, Wisniewski J, Mizuguchi G, Soule P, Mueller F, Darzacq CD, Darzacq X, Wu C, Bargmann CI, Agard DA, Dahan M, Gustafsson MG. Fast multicolor 3D imaging using aberration-corrected multifocus microscopy. Nat Methods. 2013 Jan; 10(1):60-3.
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3. Ozyamak E, Kollman J, Agard DA, Komeili A. The Bacterial Actin MamK: IN VITRO ASSEMBLY BEHAVIOR AND FILAMENT ARCHITECTURE. J Biol Chem. 2013 Feb 8; 288(6):4265-77.
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4. Schneidman-Duhovny D, Rossi A, Avila-Sakar A, Kim SJ, Velázquez-Muriel J, Strop P, Liang H, Krukenberg KA, Liao M, Kim HM, Sobhanifar S, Dötsch V, Rajpal A, Pons J, Agard DA, Cheng Y, Sali A. A method for integrative structure determination of protein-protein complexes. Bioinformatics. 2012 Dec 1; 28(24):3282-9.
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5. Mennella V, Keszthelyi B, McDonald KL, Chhun B, Kan F, Rogers GC, Huang B, Agard DA. Subdiffraction-resolution fluorescence microscopy reveals a domain of the centrosome critical for pericentriolar material organization. Nat Cell Biol. 2012 Nov; 14(11):1159-68.
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6. Cunningham CN, Southworth DR, Krukenberg KA, Agard DA. The conserved arginine 380 of Hsp90 is not a catalytic residue, but stabilizes the closed conformation required for ATP hydrolysis. Protein Sci. 2012 Aug; 21(8):1162-71.
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7. Kraemer JA, Erb ML, Waddling CA, Montabana EA, Zehr EA, Wang H, Nguyen K, Pham DS, Agard DA, Pogliano J. A phage tubulin assembles dynamic filaments by an atypical mechanism to center viral DNA within the host cell. Cell. 2012 Jun 22; 149(7):1488-99.
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8. Kelch BA, Salimi NL, Agard DA. Functional modulation of a protein folding landscape via side-chain distortion. Proc Natl Acad Sci U S A. 2012 Jun 12; 109(24):9414-9.
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9. Shao L, Winoto L, Agard DA, Gustafsson MG, Sedat JW. Interferometer-based structured-illumination microscopy utilizing complementary phase relationship through constructive and destructive image detection by two cameras. J Microsc. 2012 Jun; 246(3):229-36.
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10. Arigovindan M, Sedat JW, Agard DA. Effect of depth dependent spherical aberrations in 3D structured illumination microscopy. Opt Express. 2012 Mar 12; 20(6):6527-41.
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11. Li S, Fernandez JJ, Marshall WF, Agard DA. Three-dimensional structure of basal body triplet revealed by electron cryo-tomography. EMBO J. 2012 Feb 1; 31(3):552-62.
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12. Street TO, Lavery LA, Verba KA, Lee CT, Mayer MP, Agard DA. Cross-monomer substrate contacts reposition the Hsp90 N-terminal domain and prime the chaperone activity. J Mol Biol. 2012 Jan 6; 415(1):3-15.
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13. Kollman JM, Merdes A, Mourey L, Agard DA. Microtubule nucleation by ?-tubulin complexes. Nat Rev Mol Cell Biol. 2011 Nov; 12(11):709-21.
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14. Alexander LD, Partridge JR, Agard DA, McAlpine SR. A small molecule that preferentially binds the closed conformation of Hsp90. Bioorg Med Chem Lett. 2011 Dec 1; 21(23):7068-71.
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15. Guillet V, Knibiehler M, Gregory-Pauron L, Remy MH, Chemin C, Raynaud-Messina B, Bon C, Kollman JM, Agard DA, Merdes A, Mourey L. Crystal structure of ?-tubulin complex protein GCP4 provides insight into microtubule nucleation. Nat Struct Mol Biol. 2011 Aug; 18(8):915-9.
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16. Southworth DR, Agard DA. Client-loading conformation of the Hsp90 molecular chaperone revealed in the cryo-EM structure of the human Hsp90:Hop complex. Mol Cell. 2011 Jun 24; 42(6):771-81.
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17. Gopalakrishnan J, Mennella V, Blachon S, Zhai B, Smith AH, Megraw TL, Nicastro D, Gygi SP, Agard DA, Avidor-Reiss T. Sas-4 provides a scaffold for cytoplasmic complexes and tethers them in a centrosome. Nat Commun. 2011; 2:359.
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18. Street TO, Lavery LA, Agard DA. Substrate binding drives large-scale conformational changes in the Hsp90 molecular chaperone. Mol Cell. 2011 Apr 8; 42(1):96-105.
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19. Zheng SQ, Branlund E, Kesthelyi B, Braunfeld MB, Cheng Y, Sedat JW, Agard DA. A distributed multi-GPU system for high speed electron microscopic tomographic reconstruction. Ultramicroscopy. 2011 Jul; 111(8):1137-43.
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20. Krukenberg KA, Street TO, Lavery LA, Agard DA. Conformational dynamics of the molecular chaperone Hsp90. Q Rev Biophys. 2011 May; 44(2):229-55.
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