Dieter C. Gruenert, PhD

A B C D E F G H I J K L M N O P Q R S T U V W X Y Z
Dieter C. Gruenert, PhD

Adjunct Professor, Department of Otolaryngology–Head and Neck Surgery, UCSF

dgruenert@ohns.ucsf.edu

Phone: (415) 476-8586
Box 1330, UCSF
San Francisco, CA 94143-1330

View on UCSF Profiles

Cancer Center Membership

Associate Member » Breast Oncology

Education

University of Wisconsin, Madison, B.A., 1972, Molecular Biology
University of California, Berkeley, Ph.D., 1982, Biophysics
Institut Suisse de Recherches, Experimentales sur le Cancer, Epalinges/Lausanne, Suisse, Postdoc, 1982-1984, Carcinogenesis


Professional Experience

  • 1984-1986
    Molecular Biologist, Therapeutic Division, Hana Biologics, Inc., Berkeley, CA
  • 1986-1989
    Assistant Research Biochemist, Cardiovascular Research Institute, UCSF
  • 1990-2000
    Assistant Professor, Associate Professor, and Professor, Depts. of Lab Medicine and Stomatology, UCSF
  • 1990-2000
    Member Graduate Group in Biophysics, UCSF
  • 1991-2000
    Associate Staff, Cardiovascular Research Institute, UCSF
  • 1993-2000
    Associate Director, Gene Therapy Core Center, UCSF
  • 1996-2000
    Member, Program in Biomedical Sciences, UCSF
  • 1999
    Visiting Professor, Tor Vergata Univ., Dept. of Biopathology and Diag. Imaging, Rome, Italy
  • 2000-2003
    Member, Vermont Cancer Center
  • 2000-2003
    Director and Professor, Human Molecular Genetics, Department of Medicine, Univ of Vermont
  • 2003-2010
    Senior Scientist, California Pacific Medical Center Research Institute, San Francisco, CA
  • 2005-2010
    Head, Stem Cell Research Program, California Pacific Medical Center Research Institute, San Francisco, CA
  • 2005-2010
    Chair, SCRO Committee, California Pacific Medical Center Research Institute, San Francisco, CA
  • 2003-present
    Adjunct Professor, Department of Medicine, University of Vermont
  • 2004-present
    Adjunct Professor, Dept of Laboratory Medicine, University of California, San Francisco
  • 2006-present
    Visiting Professor, Tor Vergata Univ., Dept. of Biopathology and Diag. Imaging, Rome, Italy
  • 2009
    European Respiratory Soc Visiting Professor, Necker Hospital for Sick Children, Paris, France
  • 2010-present
    Adjunct Professor, Department of Otolaryngology–Head and Neck Surgery, UCSF

Selected Publications

  • Kunzelmann K, Legendre J-Y, Kneoll D, Escobar LC, Xu Z, Gruenert DC. Gene targeting of CFTR DNA in CF epithelial cells. Gene Therapy, 3:859-867, 1996 PMID: 8908499.
  • Goncz KK, Kunzelmann K, Xu Z, Gruenert DC. Targeted replacement of normal and mutant CFTR sequences in human airway epithelial cells using small DNA fragments. Hum Molec Genet 7:1913-1919, 1998. PMID: 9811935
  • Holmes AR, Dohrman AF, Ellison AR, Goncz KK, Gruenert DC. Intracellular Compartmentalization of DNA fragments in cultured airway epithelial cells mediated by cationic lipids. Pharm Res 16:1020-1025, 1999. PMID: 10450925
  • Goncz KK. Gruenert DC. Site-directed alteration of genomic DNA by small-fragment homologous replacement. Methods in Molecular Biology. 133:85-99, 2000. PMID: 10561833
  • Goncz KK, Colosimo A, Weiner-Kronish J, Papahadjoupolos D, Sawa T, Schreier H, Wei-wen L, Xu Z, Gruenert DC. Expression of mutant CFTR in normal mouse lung after site-specific altering of CFTR sequences by SFHR. Gene Ther 8:961-965, 2001. PMID: 11426337
  • Colosimo A, Goncz KK, Novelli G, Dallapiccola B, Gruenert DC. SFHR-mediated gene targeting of expression vector systems in human airway epithelial cells. Mol Ther 3:178-85, 2001. PMID: 11237674
  • Goncz KK, Prokopishyn NL, Chow BL, Davis BR, Gruenert DC. Application of SFHR to gene therapy of monogenic disorders. Gene Therapy 9:691-694, 2002. PMID: 12032689
  • Sangiuolo F, Bruscia E, Serafino A, Nardone AM, Bonifazi E, Lais M, Gruenert DC, Novelli G. In vitro correction of cystic fibrosis epithelial cell lines by small fragment homologous replacement (SFHR) technique. BMC Med Genet. 3:8, 2002. PMID: 12243649
  • Bruscia E, Sangiuolo F, Goncz KK, Novelli G, Gruenert DC. Isolation of CF cell lines corrected at deltaF508-CFTR locus by SFHR-mediated targeting. Gene Therapy 9:683-685, 2002. PMID: 12032687
  • Gruenert DC, Bruscia E, Novelli G, Colosimo A, Dallapiccola B, Sangiuolo F, Goncz KK. Sequence specific modification of genomic DNA by small DNA fragments. J Clin Invest, 112:637-641, 2003. PMID: 12952908
  • Goncz KK, Prokopishyn NL, Abdolmohammadi A, Bedayat B, Maurisse R, Davis BR, Gruenert DC. SFHR-mediated modification of genomic ß-globin sequences in human hematopoietic stem/progenitor cells. Oligonucleotides 16:213-224, 2006. PMID: 16978085
  • Maurisse R, Fichou Y, de Semir D, Cheung J, Ferec C, Gruenert DC. Gel Purification of Genomic DNA Removes Contaminating Small DNA Fragments Interfering with PCR Analysis of SFHR. Oligonucleotides16:375-386, 2006. PMID: 17155912
  • Sangiuolo F, Scaldaferri ML, Serafino AL, Bruscia E, Mango R, Gruenert DC, DeFelici M, Novelli G. CFTR gene targeting in mouse embryonic stem cells mediated by Small Fragment Homologous Replacement (SFHR). Frontiers Biosci 13:2989-2999, 2008. PMID: 17981772
  • Bedayat B, Abdolmohammadi A, Ye L, Maurisse R, Parsi H, Schwarz J, Emamekhoo H, Nicklas JA, O’Neill JP, Gruenert DC. Sequence-Specific Correction of Genomic HPRT Mutations in Lymphoblasts by Small Fragment Homologous Replacement (SFHR). Oligonucleotides 20:7-16, 2010. PMID: 19995283
  • Maurisse R, De Semir D, Emamekhoo H, Bedayat B, Abdolmohammadi A, Parsi H, Gruenert DC: Comparative transfection of DNA into primary and transformed mammalian cells from different lineages. BMC Biotechnol 10:9 2010. PMID: 20144189