Dieter C. Gruenert, PhD

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Education

University of Wisconsin, Madison, B.A., 1972, Molecular Biology
University of California, Berkeley, Ph.D., 1982, Biophysics
Institut Suisse de Recherches, Experimentales sur le Cancer, Epalinges/Lausanne, Suisse, Postdoc, 1982-1984, Carcinogenesis

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Professional Experience

• 1984-1986
  Molecular Biologist, Therapeutic Division, Hana Biologics, Inc., Berkeley, CA
• 1986-1989
  Assistant Research Biochemist, Cardiovascular Research Institute, UCSF
• 1990-2000
  Assistant Professor, Associate Professor, and Professor, Depts. of Lab Medicine and Stomatology, UCSF
• 1990-2000
  Member Graduate Group in Biophysics, UCSF
• 1991-2000
  Associate Staff, Cardiovascular Research Institute, UCSF
• 1993-2000
  Associate Director, Gene Therapy Core Center, UCSF
• 1996-2000
  Member, Program in Biomedical Sciences, UCSF
• 1999
  Visiting Professor, Tor Vergata Univ., Dept. of Biopathology and Diag. Imaging, Rome, Italy
• 2000-2003
  Member, Vermont Cancer Center
• 2000-2003
  Director and Professor, Human Molecular Genetics, Department of Medicine, Univ of Vermont
• 2003-2010
  Senior Scientist, California Pacific Medical Center Research Institute, San Francisco, CA
• 2005-2010
  Head, Stem Cell Research Program, California Pacific Medical Center Research Institute, San Francisco, CA
• 2005-2010
  Chair, SCRO Committee, California Pacific Medical Center Research Institute, San Francisco, CA
• 2003-present
  Adjunct Professor, Department of Medicine, University of Vermont
• 2004-present
  Adjunct Professor, Dept of Laboratory Medicine, University of California, San Francisco
• 2006-present
  Visiting Professor, Tor Vergata Univ., Dept. of Biopathology and Diag. Imaging, Rome, Italy
• 2009
  European Respiratory Soc Visiting Professor, Necker Hospital for Sick Children, Paris, France
• 2010-present
  Adjunct Professor, Department of Otolaryngology–Head and Neck Surgery, UCSF

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Selected Publications

1. Kelly C, Canning P, Buchanan PJ, Williams MT, Brown V, Gruenert DC, Elborn JS, Ennis M, Schock BC. Toll-like receptor 4 is not targeted to the lysosome in cystic fibrosis airway epithelial cells. Am J Physiol Lung Cell Mol Physiol. 2013 Mar; 304(5):L371-82.
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2. Gonzalez-Hilarion S, Beghyn T, Jia J, Debreuck N, Berte G, Mamchaoui K, Mouly V, Gruenert DC, Déprez B, Lejeune F. Rescue of nonsense mutations by amlexanox in human cells. Orphanet J Rare Dis. 2012; 7:58.
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3. Nimishakavi S, Besprozvannaya M, Raymond WW, Craik CS, Gruenert DC, Caughey GH. Activity and inhibition of prostasin and matriptase on apical and basolateral surfaces of human airway epithelial cells. Am J Physiol Lung Cell Mol Physiol. 2012 Jul; 303(2):L97-106.
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4. Mizunoe S, Shuto T, Suzuki S, Matsumoto C, Watanabe K, Ueno-Shuto K, Suico MA, Onuki K, Gruenert DC, Kai H. Synergism between interleukin (IL)-17 and Toll-like receptor 2 and 4 signals to induce IL-8 expression in cystic fibrosis airway epithelial cells. J Pharmacol Sci. 2012; 118(4):512-20.
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5. Gruenert DC, Sargent RG. Virus-mediated Genetic Surgery: Homologous Recombination With a Little "Helper" From My Friends. Mol Ther Nucleic Acids. 2012; 1:e2.
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6. de Semir D, Maurisse R, Du F, Xu J, Yang X, Illek B, Gruenert DC. Generation of SV40-transformed rabbit tracheal-epithelial-cell-derived blastocyst by somatic cell nuclear transfer. Cell Tissue Res. 2012 Feb; 347(2):357-67.
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7. John G, Chillappagari S, Rubin BK, Gruenert DC, Henke MO. Reduced surface toll-like receptor-4 expression and absent interferon-?-inducible protein-10 induction in cystic fibrosis airway cells. Exp Lung Res. 2011 Aug; 37(6):319-26.
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8. Sargent RG, Kim S, Gruenert DC. Oligo/polynucleotide-based gene modification: strategies and therapeutic potential. Oligonucleotides. 2011 Mar-Apr; 21(2):55-75.
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9. Illek B, Lei D, Fischer H, Gruenert DC. Sensitivity of chloride efflux vs. transepithelial measurements in mixed CF and normal airway epithelial cell populations. Cell Physiol Biochem. 2010; 26(6):983-90.
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10. LeSimple P, Liao J, Robert R, Gruenert DC, Hanrahan JW. Cystic fibrosis transmembrane conductance regulator trafficking modulates the barrier function of airway epithelial cell monolayers. J Physiol. 2010 Apr 15; 588(Pt 8):1195-209.
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11. Maurisse R, De Semir D, Emamekhoo H, Bedayat B, Abdolmohammadi A, Parsi H, Gruenert DC. Comparative transfection of DNA into primary and transformed mammalian cells from different lineages. BMC Biotechnol. 2010; 10:9.
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12. Bedayat B, Abdolmohamadi A, Ye L, Maurisse R, Parsi H, Schwarz J, Emamekhoo H, Nicklas JA, O'Neill JP, Gruenert DC. Sequence-specific correction of genomic hypoxanthine-guanine phosphoribosyl transferase mutations in lymphoblasts by small fragment homologous replacement. Oligonucleotides. 2010 Feb; 20(1):7-16.
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13. Treharne KJ, Xu Z, Chen JH, Best OG, Cassidy DM, Gruenert DC, Hegyi P, Gray MA, Sheppard DN, Kunzelmann K, Mehta A. Inhibition of protein kinase CK2 closes the CFTR Cl channel, but has no effect on the cystic fibrosis mutant deltaF508-CFTR. Cell Physiol Biochem. 2009; 24(5-6):347-60.
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14. John G, Yildirim AO, Rubin BK, Gruenert DC, Henke MO. TLR-4-mediated innate immunity is reduced in cystic fibrosis airway cells. Am J Respir Cell Mol Biol. 2010 Apr; 42(4):424-31.
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15. Barriere H, Bagdany M, Bossard F, Okiyoneda T, Wojewodka G, Gruenert D, Radzioch D, Lukacs GL. Revisiting the role of cystic fibrosis transmembrane conductance regulator and counterion permeability in the pH regulation of endocytic organelles. Mol Biol Cell. 2009 Jul; 20(13):3125-41.
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16. Griesenbach U, Meng C, Farley R, Gardner A, Brake MA, Frankel GM, Gruenert DC, Cheng SH, Scheule RK, Alton EW. The role of doxorubicin in non-viral gene transfer in the lung. Biomaterials. 2009 Apr; 30(10):1971-7.
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17. Schwarzer C, Fischer H, Kim EJ, Barber KJ, Mills AD, Kurth MJ, Gruenert DC, Suh JH, Machen TE, Illek B. Oxidative stress caused by pyocyanin impairs CFTR Cl(-) transport in human bronchial epithelial cells. Free Radic Biol Med. 2008 Dec 15; 45(12):1653-62.
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18. Sajjan U, Wang Q, Zhao Y, Gruenert DC, Hershenson MB. Rhinovirus disrupts the barrier function of polarized airway epithelial cells. Am J Respir Crit Care Med. 2008 Dec 15; 178(12):1271-81.
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19. Treharne KJ, Crawford RM, Xu Z, Chen JH, Best OG, Schulte EA, Gruenert DC, Wilson SM, Sheppard DN, Kunzelmann K, Mehta A. Protein kinase CK2, cystic fibrosis transmembrane conductance regulator, and the deltaF508 mutation: F508 deletion disrupts a kinase-binding site. J Biol Chem. 2008 Sep 5; 283(36):25103.
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20. Illek B, Maurisse R, Wahler L, Kunzelmann K, Fischer H, Gruenert DC. Cl transport in complemented CF bronchial epithelial cells correlates with CFTR mRNA expression levels. Cell Physiol Biochem. 2008; 22(1-4):57-68.
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