Arun P. Wiita, MD, PhD

A B C D E F G H I J K L M N O P Q R S T U V W X Y Z
Arun P. Wiita, MD, PhD

Health Sciences Clinical Instructor, Dept. of Laboratory Medicine, UCSF

Box 0134, UCSF
San Francisco, CA 94158

UCSF Profiles

Cancer Center Membership

Associate Member » Hematopoietic Malignancies

Research Summary

My research laboratory spans a number of areas within genomics, proteomics, and hematologic malignancies. One major area of research involves the use of targeted, quantitative proteomics to answer important biological questions in myeloma and B-cell leukemia. We are particularly interested in questions related to translational regulation after cancer therapy as well as how clinically-relevant genomic alterations result in phenotypic changes at the level of the proteome. Furthermore, my group includes the UCSF Stephen and Nancy Grand Multiple Myeloma Translational Initiative Laboratory. In this role, we collaborate with industry and academic partners to advance preclinical evaluation of novel myeloma therapeutics using a suite of in vitro and in vivo approaches.

In my clinical work, I am Assistant Director of the UCSF Clinical Cytogenetics laboratory. In this role, I interpret molecular genetic testing for the prenatal and postnatal diagnosis of microdeletion and microduplication syndromes. In this area we have translational interests in emerging technologies for determination of DNA copy number variation, integration of single-nucleotide variants and CNV interpretation, and cell-free DNA for prenatal testing. In my research laboratory we are also using quantitative proteomics to evaluate the role of protein homeostasis in determining the phenotypic effects of CNVs, as well using CRISPR/Cas9 genome engineering to generate new functional models of syndromic CNVs.

Education

2002, Princeton University, Princeton, NJ, A.B., Chemistry
2008, Columbia University, New York, NY, Ph.D, Neurobiology/Biophysics
2009, Columbia University, New York, NY, M.D., Medicine
2012, University of California, San Francisco, CA, Residency, Laboratory Medicine
2014, University of California, San Francisco, CA, Post-Doc, Pharmaceutical Chemistry


Professional Experience

  • 2007
    Graduate Student Fellow, Columbia U. Science & Technology Venture
  • 2008-2009
    Co-founder, MedVolution Systems, Inc. (clinical decision support software)
  • 2009-2012
    Resident, Dept. of Laboratory Medicine, UCSF
  • 2012-2014
    Post-doctoral Scholar, Wells Lab, Dept. of Pharmaceutical Chemistry, UCSF
  • 2014-present
    HS Clinical Instructor, Dept. of Laboratory Medicine, UCSF

Honors & Awards

  • 2008
    Winner, Wharton Venture Award, team “Bayesian Diagnosis Pro”, U. Penn.
  • 2008
    Dean’s Award for Excellence in Research, Columbia U. College of P&S (best Ph.D. thesis prize)
  • 2010, 2013
    Paul Strandjord Young Investigator Award, Academy of Clinical & Lab. Physicians and Scientists
  • 2010
    Laurence J. Marton Award for Excellence in Research, Dept. Lab. Med., UCSF
  • 2010
    Julius Krevans Award for Clinical Excellence, San Francisco General Hospital/UCSF
  • 2012
    NIH Ruth F. Kirschstein F32 Post-doctoral Fellowship (declined)
  • 2012
    Jane Coffin Childs Fund Post-doctoral Fellowship (declined)
  • 2012-15
    Damon Runyon Cancer Research Foundation Post-doctoral Fellowship

Selected Publications

  1. Sherbenou DW, Aftab BT, Su Y, Behrens CR, Wiita A, Logan AC, Acosta-Alvear D, Hann BC, Walter P, Shuman MA, Wu X, Atkinson JP, Wolf JL, Martin TG, Liu B. Antibody-drug conjugate targeting CD46 eliminates multiple myeloma cells. J Clin Invest. 2016 Nov 14.
    View on PubMed
  2. Mather CA, Qi Z, Wiita AP. False positive cell free DNA screening for microdeletions due to non-pathogenic copy number variants. Prenat Diagn. 2016 Jun; 36(6):584-6.
    View on PubMed
  3. Ori-McKenney KM, McKenney RJ, Huang HH, Li T, Meltzer S, Jan LY, Vale RD, Wiita AP, Jan YN. Phosphorylation of ß-Tubulin by the Down Syndrome Kinase, Minibrain/DYRK1a, Regulates Microtubule Dynamics and Dendrite Morphogenesis. Neuron. 2016 May 4; 90(3):551-63.
    View on PubMed
  4. Julien O, Zhuang M, Wiita AP, O'Donoghue AJ, Knudsen GM, Craik CS, Wells JA. Quantitative MS-based enzymology of caspases reveals distinct protein substrate specificities, hierarchies, and cellular roles. Proc Natl Acad Sci U S A. 2016 Apr 5; 113(14):E2001-10.
    View on PubMed
  5. Wiita AP, Hsu GW, Lu CM, Esensten JH, Wells JA. Circulating proteolytic signatures of chemotherapy-induced cell death in humans discovered by N-terminal labeling. Proc Natl Acad Sci U S A. 2014 May 27; 111(21):7594-9.
    View on PubMed
  6. Carr SA, Abbatiello SE, Ackermann BL, Borchers C, Domon B, Deutsch EW, Grant RP, Hoofnagle AN, Hüttenhain R, Koomen JM, Liebler DC, Liu T, MacLean B, Mani DR, Mansfield E, Neubert H, Paulovich AG, Reiter L, Vitek O, Aebersold R, Anderson L, Bethem R, Blonder J, Boja E, Botelho J, Boyne M, Bradshaw RA, Burlingame AL, Chan D, Keshishian H, Kuhn E, Kinsinger C, Lee JS, Lee SW, Moritz R, Oses-Prieto J, Rifai N, Ritchie J, Rodriguez H, Srinivas PR, Townsend RR, Van Eyk J, Whiteley G, Wiita A, Weintraub S. Targeted peptide measurements in biology and medicine: best practices for mass spectrometry-based assay development using a fit-for-purpose approach. Mol Cell Proteomics. 2014 Mar; 13(3):907-17.
    View on PubMed
  7. Wiita AP, Seaman JE, Wells JA. Global analysis of cellular proteolysis by selective enzymatic labeling of protein N-termini. Methods Enzymol. 2014; 544:327-58.
    View on PubMed
  8. Wiita AP, Ziv E, Wiita PJ, Urisman A, Julien O, Burlingame AL, Weissman JS, Wells JA. Global cellular response to chemotherapy-induced apoptosis. Elife. 2013; 2:e01236.
    View on PubMed
  9. Wiita AP, Nambiar A. Longitudinal management with crossmatch-compatible platelets for refractory patients: alloimmunization, response to transfusion, and clinical outcomes (CME). Transfusion. 2012 Oct; 52(10):2146-54.
    View on PubMed
  10. Wiita AP, Roubinian N, Khan Y, Chin-Hong PV, Singer JP, Golden JA, Miller S. Cytomegalovirus disease and infection in lung transplant recipients in the setting of planned indefinite valganciclovir prophylaxis. Transpl Infect Dis. 2012 Jun; 14(3):248-58.
    View on PubMed
  11. Wiita AP, Schrijver I. Clinical application of high throughput molecular screening techniques for pharmacogenomics. Pharmgenomics Pers Med. 2011; 4:109-21.
    View on PubMed
  12. Perez-Jimenez R, Li J, Kosuri P, Sanchez-Romero I, Wiita AP, Rodriguez-Larrea D, Chueca A, Holmgren A, Miranda-Vizuete A, Becker K, Cho SH, Beckwith J, Gelhaye E, Jacquot JP, Gaucher EA, Gaucher E, Sanchez-Ruiz JM, Berne BJ, Fernandez JM. Diversity of chemical mechanisms in thioredoxin catalysis revealed by single-molecule force spectroscopy. Nat Struct Mol Biol. 2009 Aug; 16(8):890-6.
    View on PubMed
  13. Perez-Jimenez R, Wiita AP, Rodriguez-Larrea D, Kosuri P, Gavira JA, Sanchez-Ruiz JM, Fernandez JM. Force-clamp spectroscopy detects residue co-evolution in enzyme catalysis. J Biol Chem. 2008 Oct 3; 283(40):27121-9.
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  14. Koti Ainavarapu SR, Wiita AP, Dougan L, Uggerud E, Fernandez JM. Single-molecule force spectroscopy measurements of bond elongation during a bimolecular reaction. J Am Chem Soc. 2008 May 21; 130(20):6479-87.
    View on PubMed
  15. Ainavarapu SR, Wiita AP, Huang HH, Fernandez JM. A single-molecule assay to directly identify solvent-accessible disulfide bonds and probe their effect on protein folding. J Am Chem Soc. 2008 Jan 16; 130(2):436-7.
    View on PubMed
  16. Szoszkiewicz R, Ainavarapu SR, Wiita AP, Perez-Jimenez R, Sanchez-Ruiz JM, Fernandez JM. Dwell time analysis of a single-molecule mechanochemical reaction. Langmuir. 2008 Feb 19; 24(4):1356-64.
    View on PubMed
  17. Wiita AP, Perez-Jimenez R, Walther KA, Gräter F, Berne BJ, Holmgren A, Sanchez-Ruiz JM, Fernandez JM. Probing the chemistry of thioredoxin catalysis with force. Nature. 2007 Nov 1; 450(7166):124-7.
    View on PubMed
  18. Ainavarapu SR, Brujic J, Huang HH, Wiita AP, Lu H, Li L, Walther KA, Carrion-Vazquez M, Li H, Fernandez JM. Contour length and refolding rate of a small protein controlled by engineered disulfide bonds. Biophys J. 2007 Jan 1; 92(1):225-33.
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  19. Wiita AP, Ainavarapu SR, Huang HH, Fernandez JM. Force-dependent chemical kinetics of disulfide bond reduction observed with single-molecule techniques. Proc Natl Acad Sci U S A. 2006 May 9; 103(19):7222-7.
    View on PubMed
  20. Kipp RA, Case MA, Wist AD, Cresson CM, Carrell M, Griner E, Wiita A, Albiniak PA, Chai J, Shi Y, Semmelhack MF, McLendon GL. Molecular targeting of inhibitor of apoptosis proteins based on small molecule mimics of natural binding partners. Biochemistry. 2002 Jun 11; 41(23):7344-9.
    View on PubMed

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