Andrew Wyrobek, PhD

Andrew Wyrobek, PhD

Senior Staff Scientist, Lawrence Berkeley National Laboratory

Phone: (510) 989-4466 (voice)
Lawrence Berkeley Natl Laboratory
Life Sciences Division, One Cyclotron Road
Berkeley, CA 94270-8268


University of Notre Dame, Indiana, BS, 1970, Physics (cum laude)
Ontario Cancer Institute, University of Toronto, Canada, PhD, 1975, Medical Biophysics (with honors)

Professional Experience

  • 1973
    Medical Biophysicist, Chemical Hazards Branch, Toxic Hazards Division, Aerospace Medical Research Laboratory, Wright-Patterson Air Force Base, Ohio
  • 1975-2006
    Senior Staff Medical Biophysicist, Biology and Biotechnology Research Program, Lawrence Livermore Laboratory (LLNL); 1975-1987, Reproductive Biology Section; 1987-1991, Molecular Biology Section; 1991-1995, Biodosimetry Group
  • 1976-1999
    Visiting Lecturer, School of Public Health, University of California, Berkeley
  • 1976-1996
    Visiting Lecturer, San Jose State University, San Jose, California
  • 1970-1982
    Captain and Lieutenant, US Air Force Reserves
  • 1995-2002
    Team Leader, Genetic Damage and Disease Team, LLNL
  • 2002-2005
    Division Leader, Health Effects Genetics Division, LLNL
  • 1995-2005
    Chair, Institutional Review Board (Human Subjects Committee), LLNL
  • 2004-2006
    Senior Biomedical Research Scientist, School of Public Health, University of California, Berkeley
  • 1997-2006
    Executive Committee, University of California BioDiscovery Program
  • 2006-present
    Senior Staff Scientist, Lawrence Berkeley National Laboratory, Berkeley CA

Honors & Awards

  • 2006-present
    President-elect, Environmental Mutagen Society
  • 2006-present
    Member, National Council for Radiation Protection

Selected Publications

  • Mascio, L.N., Yuen, B.K., Kegelmeyer, W.P. Jr., Matsumoto, K., Briner, J., and Wyrobek, A.J. (1998). Advances in the automated detection of metaphase chromosomes labeled with fluorescence dyes. Cytometry 33:10-18.
  • Baumgartner A., Van Hummelen P., Lowe X., Adler I.-D., and Wyrobek A.J. (1999) Numerical and structural chromosomal abnormalities detected in human sperm with a combination of multicolor FISH assays. Env Mol Mut 33:49-58.
  • Titenko-Holland, N., Ahlborn, T., Turteltaub, K., Markee, C., Moore II, D., Wyrobek, A.J., and Smith, M.T. (1999). Acrylamide causes preimplantation in male germ of mice. Reprod Toxicol 13, 167-178.
  • Marchetti, F., Lowe, X., Bishop, J., and Wyrobek, A.J. (1999). Absence of selection against aneuploid sperm during spermiogenesis, fertilization and the first cell-cycle of mammalian zygotes Biol Reprod 61, 948-954 (1999).
  • Sloter, E., Lowe, X., Moore, D. II, Nath, J., Wyrobek, A. (2000) Multicolor FISH analysis of Chromosomal Breaks, Duplications, Deletions, and numerical abnormalities in the sperm of healthy men.Am J Hum Genet 67:862-72.
  • Wyrobek, A. J., Marchetti, F., Sloter, E., Bishop, J. (2000) Chromosomally defective sperm and their developmental consequences. In: Anderson D, Karakaya AE, Sram RJ (eds) "Human Monitoring after Environmental and Occupational Exposure to Chemical and Physical Agents", NATO Science Series, Vol. 313, IOS Press, pp 134-150.
  • Kidd, SA, Eskenazi, B. Wyrobek, A.J. (2001) The Effects of male age on semen quality: a review of the literature. Fertil Steril 75:237-248.
  • Kegelmeyer, L.M., Tomascik-Cheeseman L.M., Burnett, M.S., van Hummelen, P., Wyrobek, A.J., (2001) A groundtruth approach to accurate quantitation of fluorescence microarrays. SPIE Proceedings (4266).
  • Marchetti F., Sloter, E., Wyrobek , A.J. (2001) Advances in understanding paternally transmitted chromosomal abnormalities. In: Sram RJ (Ed) "Male reproductive studies in the Teplice Program", Academia, Prague, Czech Republic, pp 193-205.
  • Schmid, T.E., Lowe, X., Marchetti, F., Bishop, J., Haseman, J., Wyrobek, A.J. (2001) Evaluation of inter-scorer and inter-laboratory reliability of the mouse epididymal sperm aneuploidy (m-ESA) assay. Mutagenesis 16, 189-195.
  • Marchetti, F., Bishop, J.B., Lowe, X., Generoso, W.M., Hozier , J., Wyrobek, A.J. (2001) Etoposide induces heritable chromosomal aberrations and aneuploidy during male meiosis in the mouse. Proc Natl Acad Sci USA 98, 3952-3957.
  • Lowe, X., Eskenazi, B., Nelson, D.O., Kidd, S., Alme, A., Wyrobek, A.J. (2001) Frequency of XY sperm increases with age in fathers of boys with Klinefelter Syndrome. Am J Hum Genet 69, 1046-1054.
  • Eskenazi, B., Wyrobek A.J., Kidd, S.A., Lowe, X., Moore D.II, Weisinger, K., Aylstock, M. (2002) Sperm aneuploidy in fathers of children with paternally and maternally inherited Klinefelter syndrome. Human Reprod 17(3):576-83.
  • Tomascik-Cheeseman, L.M., Lowe, X., Marchetti,, F., Shamanski FL, Pedersen R, Nath J Wyrobek AJ (2002) CENP-B is not critical for meiotic segregation in male mice. Mutation Res 513, 197-203.
  • Rubes, J., Vozdova, M., Robbins W.A., Rezacova, O., Perreault, S.D., Wyrobek A.J. (2002) Stable variants of sperm aneuploidy among healthy men show associations between germinal and somatic aneuploidy. Am. J. Hum. Genet. 70, 1507-1519.
  • Sorenson, K.J., Attix C.M., Christian, A.T., Wyrobek, A.J., Tucker, J.D. (2002) Adaptive response induction and variation in human lymphoblastoid cell lines. Mutation Res 519:15-24.
  • Tiemann-Boege, I., Navidi, W., Grewal, R., Cohn, D., Eskenazi, B., Wyrobek, A.J., Arnheim, N. (2002) The observed human sperm mutation frequency cannot explain the achondroplasia paternal age effect. PNAS Nov 12;99(23):14952-7.
  • Frias, S., VanHummelen, P., Meistrich M.L., Lowe X.R., Hagemeister F.B., Shelby, M.D., Bishop J.B., Wyrobek A.J., (2003) NOVP chemotherapy for Hodgkins disease transiently induces sperm aneuploidies associated with the major clinical aneuploidy syndromes. Cancer Research. Jan 1;63(1):44-51.
  • Eskenazi, B., Wyrobek, A.J., Sloter, E., Kidd, S.A., Moore, L., Young, S.S., Moore D. II (2003). The effect of age on semen quality in healthy men. Human Reprod 18(2):447-54.
  • Perreault, S.D, Aitken, R.J., Baker, H.W., Everson, D.P., Huszar, G., Irvine, D.S., Morris, I.D., Morris, R., Robbins, W.A., Sakkas, D., Spano, M., Wyrobek, A.J. (2003) Integrating new tests of sperm genetic integrity into semen analysis: breakout group discussion. In: Advances in Male Mediated Developmental Toxicity (B. Robaire and B. Hales, Eds.) Kluwer Academic/Plenum Publisher, New York, Vol. 518 pp 253-268
  • Marchetti, F., Wyrobek, A.J. (2003) PAINT/DAPI analysis of mouse zygotes to detect paternally transmitted chromosomal damage. In: Advances in Male Mediated Develpmental Toxicity (B. Robaire and B. Hales, Eds.) Kluwer Academic/Plenum Publisher, New York, Vol. 518, pp 131-145.
  • Barton TS, Wyrobek AJ, Hill FS, Robaire B, Hales BF. (2003) Numerical chromosomal abnormalities in rat epididymal spermatozoa following chronic cyclophosphamide exposure. Biol Reprod. 69(4):1150-7.
  • Marchetti F, Wyrobek AJ. (2003) PAINT/DAPI analysis of mouse zygotes to detect paternally transmitted chromosomal aberrations. Adv Exp Med Biol. 518:131-45.
  • Perreault SD, Aitken RJ, Baker HW, Evenson DP, Huszar G, Irvine DS, Morris ID, Morris RA, Robbins WA, Sakkas D, Spano M, Wyrobek AJ. (2003) Integrating new tests of sperm genetic integrity into semen analysis: breakout group discussion. Adv Exp Med Biol. 518:253-68.
  • Glaser RL, Broman KW, Schulman RL, Eskenazi B, Wyrobek AJ, Jabs EW. (2003) The paternal-age effect in Apert syndrome is due, in part, to the increased frequency of mutations in sperm. Am J Hum Genet. 73(4):939-47.
  • Kohane M, Daugela A, Kutomi H, Charlson L, Wyrobek A, Wyrobek J. (2003) Nanoscale in vivo evaluation of the stiffness of Drosophila melanogaster integument during development. J Biomed Mater Res A. 66:633-42.
  • Hill FS, Marchetti F, Liechty M, Bishop J, Hozier J, Wyrobek AJ. (2003) A new FISH assay to simultaneously detect structural and numerical chromosomal abnormalities in mouse sperm. Mol Reprod Dev. 66(2):172-80.
  • Marchetti F, Bishop JB, Cosentino L, Moore D 2nd, Wyrobek AJ. (2004) Paternally transmitted chromosomal aberrations in mouse zygotes determine their embryonic fate. Biol Reprod. 70(3):616-24.
  • Yin E, Nelson DO, Coleman MA, Peterson LE, Wyrobek AJ. (2003) Gene expression changes in mouse brain after exposure to low-dose ionizing radiation. Int J Radiat Biol. 79(10):759-75.
  • Sloter E, Nath J, Eskenazi B, Wyrobek AJ. (2004) Effects of male age on the frequencies of germinal and heritable chromosomal abnormalities in humans and rodents. Fertil Steril. 81(4):925-43.
  • Schmid TE, Brinkworth MH, Hill F, Sloter E, Kamischke A, Marchetti F, Nieschlag E, Wyrobek AJ. (2004) Detection of structural and numerical chromosomal abnormalities by ACM-FISH analysis in sperm of oligozoospermic infertility patients. Hum Reprod. 19(6):1395-400.
  • Tomascik-Cheeseman LM, Coleman MA, Marchetti F, Nelson DO, Kegelmeyer LM, Nath J, Wyrobek AJ. (2004) Differential basal expression of genes associated with stress response, damage control, and DNA repair among mouse tissues. Mutat Res. 561(1-2):1-14.
  • Motin VL, Georgescu AM, Fitch JP, Gu PP, Nelson DO, Mabery SL, Garnham JB, Sokhansanj BA, Ott LL, Coleman MA, Elliott JM, Kegelmeyer LM, Wyrobek AJ, Slezak TR, Brubaker RR, Garcia E. (2004) Temporal global changes in gene expression during temperature transition in Yersinia pestis. J Bacteriol. 186:6298-305.
  • Zudova D, Wyrobek AJ, Bishop J, Marchetti F. (2004) Impaired fertility in T-stock female mice after superovulation. Reproduction. 128(5):573-81.
  • Eskenazi B, Kidd SA, Marks AR, Sloter E, Block G, Wyrobek AJ. (2005) Antioxidant intake is associated with semen quality in healthy men. Hum Reprod. 20(4):1006-12.
  • Wyrobek AJ, Schmid TE, Marchetti F. (2005) Cross-species sperm-FISH assays for chemical testing and assessing paternal risk for chromosomally abnormal pregnancies. Environ Mol Mutagen. 45(2-3):271-83.
  • Wyrobek AJ , Schmid TE, Marchetti F. (2005) Relative susceptibilities of male germ cells to genetic defects induced by cancer chemotherapies. J Natl Cancer Inst Monogr. (34):31-5.
  • Silver EW, Eskenazi B, Evenson DP, Block G, Young S, Wyrobek AJ. (2005) Effect of antioxidant intake on sperm chromatin stability in healthy nonsmoking men. J Androl. 26(4):550-6.
  • Marchetti F, Wyrobek AJ. (2005) Mechanisms and consequences of paternally-transmitted chromosomal abnormalities. Birth Defects Res C Embryo Today. 75(2):112-29.
  • Coleman MA, Yin E, Peterson LE, Nelson D, Sorensen K, Tucker JD, Wyrobek AJ. (2005) Low-dose irradiation alters the transcript profiles of human lymphoblastoid cells including genes associated with cytogenetic radioadaptive response. Radiat Res. 164(4 Pt 1):369-82.
  • Marchetti F, Pearson FS, Bishop JB, Wyrobek AJ. (2005) Etoposide induces chromosomal abnormalities in mouse spermatocytes and stem cell spermatogonia. Hum Reprod. Nov 25; [Epub ahead of print]