dstokoe@cc.ucsf.edu
(415) 502-2598 (voice)
(415) 502-3179 (fax)

Box 0128, UCSF; San Francisco, CA 94143-0128

additional websites:

Stokoe Lab Website

University of Bristol, Bristol, England, B.Sc. (Honors), 1990, Biochemistry
University of Dundee, Dundee, Scotland, Ph.D., 1993, Biochemistry

Stokoe, D. and McCormick, F. (1997). Activation of Raf by Ras and Src by different mechanisms-activation in vitro and in vivo. EMBO Journal 16:2384-2392.

Stokoe, D., Stephens, L., Copeland, T., Gaffney, P. R. J., Reese, C. B., Pointer, G., Holmes, A, B. McCormick, F. and Hawkins, P. (1997). Dual role of phosphatidylinositol (3,4,5) trisphosphate in activation of protein kinase B. Science 277:567-570.

Stephens, L., Anderson, K., Stokoe, D., Erdjument-Bromage, H., Painter, G. F., Holmes, A. B., Gaffney, P. R. J., Reese, C. B., McCormick, F., Tempst, P., Coadwell, J. and Hawkins, P. T. (1998). Protein kinase B kinases that mediate phosphatidylinositol 3,4,5-trisphosphate-dependent activation of protein kinase B. Science, 279 pp 710-714.

Haas-Kogan, D., Shalev, N., Wong, M., Mills, G., Yount, G. and Stokoe, D. (1998) PKB/akt Activity is Elevated in Glioblastoma Cells Due to Mutation of PTEN/MMAC1. Current Biology, vol 8 pp1195-1198.

Kane, L. P., Shapiro, V. S., Stokoe, D. and Weiss, A. (1999) Induction of NF-kB by the Akt/PKB Kinase. Current Biology 9 601-604.

Zundel, W., Schindler, C., Haas-kogan, D., Koong, A., Kaper, F., Chen, E., Gottschaulk, A. R., Ryan, H. E., Johnson, R. S., Jefferson, A. B., Stokoe, D., Giaccia, A. J. (2000) Loss of PTEN facilitates HIF-1-mediated gene expression. Genes and Development 14 391-396.

Taylor, V. C., Wong, M., Brandts, C., Reilly, L., Dean, N. M., Cowsert, L. M., Moodie, S. and Stokoe, D. (2000) The 5Õ Inositol Phospholipid Phosphatase SHIP-2 Causes PKB Inactivation and Cell Cycle Arrest in Glioblastoma Cells. Molecular and Cellular Biology. 20 6860-6871.

Flynn, P., Wong, M., Zavar, M., Dean, N. M. and Stokoe, D. (2000) Inhibition of PDK-1 Activity Causes a Reduction in Cellular Proliferation and Survival. Current Biology, 10 1439-43.

Gottschaulk, A. R., Basila, D., Wong, M., Dean, N. M., Brandts, C. H., Stokoe, D. and Haas-Kogan, D. A. (2001) The PTEN-induced G1 growth arrest requires p27Kip1. Cancer Research, 61 2105-2111.

Stokoe, D. (2001) PTEN. Current Biology. 11 R502.

Sussman, J., Stokoe, D., Ossina, N., and Shtivelman, E. (2001) Protein kinase B phosphorylates AHNAK and regulates its subcellular localization. J Cell Biol 154 1019-30.

Ermoian, R., Furniss, C. S., Lamborn, K. R., Basila, D., Berger, M. S., Gottschalk, A. R., Nicholas, M. K., Stokoe, D. and Haas-Kogan, D. A. (2002) Dysregulation of PTEN and protein kinase B is associated with glioma histology and patient survival. Clinical Cancer Research 8 1100-6

Shtivelman, E., Sussman, J. and Stokoe, D., (2002) A role for PI3-kinase and PKB activity in the G2/M phase of the cell cycle. Current Biology 12 919-24.

Donovan, S., See, W., Bonifas, J., Stokoe, D. and Shannon, K. M. (2002) Hyperactivation of protein kinase B and ERK have discrete effects on survival, proliferation and cytokine expression in Nf1-deficient myeloid cells. Cancer Cell, 2(6) p. 507-14

Gump, J., Stokoe, D. and McCormick, F. (2003) Phosphorylation of p16INK4A correlates with Cdk4 association J. Biol. Chem. 278(9) p. 6619-22.

Stokoe, D. Protein Phosphorylation Drug Discovery Summit--SRI Conference. Targeting kinases and phosphatases for novel therapeutics. 3-5 March 2003, San Diego, CA, USA. IDrugs, 2003. 6(5): p. 432-6.

Fujii, N., Haresco, J. J., Novak, K. A., Stokoe, D., Kuntz, I. D. and Guy, R. K. (2003) A selective irreversible inhibitor targeting a PDZ protein interaction domain. J Am Chem Soc 125(40): p. 12074-5.

Hirose, Y., Katayama, M., Stokoe, D., Haas-Kogan, D. A., Berger, M. S. and Pieper, R. O. (2003) The p38 mitogen-activated protein kinase pathway links the DNA mismatch repair system to the G2 checkpoint and to resistance to chemotherapeutic DNA-methylating agents. Mol. Cell. Biol. 23(22); 8306-16

Vijipurkar, U., Fischbach, N., Shen, W., Brandts, C., Stokoe, D., Lawrence, H. J. and Largman, C. (2004) Protein Kinase C-mediated phosphorylation of the leukemia-associated HOXA9 protein impairs its DNA-binding ability and induces myeloid differentiation. Mol. Cell. Biol. 24(9); 3827-37.

Pore, N., Liu, S., Shu, H.-K., Li, B., Haas-Kogan, D., Stokoe, D., Milanini-Mongiat, J., Pages, G., OÕRourke, D. M., Bernhard, E. and Maity, A. (2004). Sp1 is involved in Akt-mediated induction of VEGF expression through a HIF-1 independent mechanism. Molecular Biology of the Cell 15(11); 4841-53.

Tiffin, N., Adi, S., Stokoe, D., Wu, N. Y. and Rosenthal, S. M. (2004). Akt phosphorylation is not sufficient for insulin-like growth factor-stimulated myogenin expression but must be accompanied by down-regulation of mitogen-activated protein kinase/extracellular signal-regulated kinase phosphorylation. Endocrinology 145(11); 4991-6.

Brandts, C., Bilanges, B., Hare, G., McCormick, F. and Stokoe, D. (2005) Phosphorylation-independent stabilization of p27kip1 by the PI3-kinase pathway in glioblastoma cells. J. Biol. Chem. 280 2012-9.

Stokoe, D. and Costello, J. (2005) Phosphatase and tensin homologue growth suppression without phosphatase. Proc. Natl. Acad. Sci. 102 2677-8.

Nakamura, J. L., Karlsson, A., Arvold, N. D., Gottschalk, A. R., Pieper, R. O., Stokoe, D. and Haas-Kogan, D. A. (2005) PKB/Akt mediates radiosensitization by the signaling inhibitor LY294002 in human malignant gliomas. J. Neurooncol. 71 215-22.

Gottschalk, A. R., Doan, A., Nakamura, J. L., Haas-Kogan, D. A. and Stokoe, D. (2005) Inhibition of phosphatidylinositol-3-kinase causes cell death through a protein kinase B (PKB)-dependent mechanism and growth arrest through a PKB-independent mechanism. Int J Radiat Oncol Biol Phys 61 1183-8.

O'Shea, C., Klupsch, K., Choi, S., Bagus, B., Soria, C., Shen, J., McCormick, F. and Stokoe, D. (2005). Adenoviral proteins mimic nutrient/growth signals to activate the mTOR pathway for viral replication. EMBO Journal, 24 1211-21.

Bilanges, B. and Stokoe, D. (2005) Direct comparison of the specificity of gene silencing using antisense and RNAi. Biochemical Journal, 388 573-83.

Haas-Kogan, D. A., Prados, M. D., Jelluma, N., Arvold, N. D., Baumber, R., Lamborn, K. R., Kapadia, A., Malec, M., Berger, M. S. and Stokoe, D. (2005) Expression Levels of EGFR and phosphorylated PKB/Akt are Associated with Response of Gliomas to the EGFR Inhibitor erlotinib (Tarceva). J. Natl. Cancer Inst., 97 880-7.

Stokoe, D. (2005) PI3-kinase pathway and cancer, Expert Reviews in Molecular Medicine, 7 1-22.

OÕShea, C., Choi, S., McCormick, F. and Stokoe, D. (2005) Adenovirus overrides cellular checkpoints for protein translation. Cell Cycle, 4 883-8.

Tominaga, T., Meyer, T., Kolesnichenko, M., Bilanges, B. and Stokoe, D. (2005) Translational Deregulation in PDK-1-/- ES Cells. Mol. Cell. Biol. 25 8465-8475.

Haas-Kogan, D. A., Prados, M. D., Lamborn, K. R., Tihan, T., Berger, M. S. and Stokoe, D. (2005) Biomarkers to predict response to EGFR Receptor inhibitors. Cell Cycle, 4 1369-72.

Gottschalk, A. R., Doan, A., Nakamura, J. L., Stokoe, D. and Haas-Kogan, D. A. (2005) Inhibition of phosphatidylinositol-3-kinase causes increased sensitivity to radiation through a PKB-dependent mechanism. Int J Radiat Oncol Biol Phys 63 1221-7.

Oda, K., Stokoe, D., Taketani, Y. and McCormick, F. (2005) High Frequency of Coexistent Mutations of PIK3CA and PTEN Genes in Endometrial Carcinoma. Cancer Research 65 10669-73.

Mellinghoff, I. K., Wang, M. Y., Vivanco, I., Haas-Kogan, D. A., Zhu, S., Dia, E. Q., Lu, K. V., Yoshimoto, K., Huang, J. H. Y., Chute, D. J., Riggs, B. L., Horvath, S., Liau, L. M., Cavenee, W. K., Rao, P. N., Beroukhim, R., Peck, T. C., Lee, J. C., Sellers, W. R., Stokoe, D., Prados, M., Cloughesy, T. F., Sawyers, C. L. and Mischel, P. (2005) Molecular determinants of EGFR kinase inhibitor response in glioblastoma. NEJM. 353 2012-24.

Fan, Q. W., Knight, Z. A., Goldenberg, D. D., Yu, W., Mostov, K. E., Stokoe, D., Shokat, K. M. and Weiss, W. A. (2006) A dual PI3 kinase/mTOR inhibitor reveals emergent efficacy in glioma Cancer Cell 9 341-9.

Knight, Z. A., Gonzalez, B., Feldman, M. E., Zunder, E. R., Goldenberg, D. D., Williams, O., Loewith, R., Stokoe, D., Balla, A., Toth, B., Balla, T., Weiss, W. A., Williams, R. L. and Shokat, K. M. (2006) A Pharmacological Map of the PI3-K Family Defines a Role for p110alpha in Insulin Signaling. Cell 125 733-47.

1/5/07

photo: Chris T. Anderson