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Genome Analysis Equipment & Software

Instrumentation

All equipment located at the Mt. Zion Campus, Cancer Research Building, 2340 Sutter Street, unless noted otherwise.

  • AB 7900HT with exchangeable blocks (96, 384-well Fast PCR, Taqman Array) and high-throughput plate loading accessory. Mt. Zion Campus, Cancer Research Building, 2340 Sutter St, Room S167.
  • AB 7900HT with exchangeable blocks (384-well Fast PCR, Taqman Array). Mission Bay Campus, Helen Diller Family Cancer Research Building, 1450 3rd St., HD-235.
  • Sequenom Compact MassARRAY System
  • Nanodrop 8000 spectrophotometer
  • Agilent 2100 Bioanalyzer
  • Two MJ Research tetrad DNA engines with four independent PCR blocks.
  • ABI 9600 PCR block with exchangeable 96- and 384-well blocks.
  • Synergy Biotek Plate Reader
  • Nanodrop-8000
  • AB 7900HT with 384-well Fast PCR Block and exchangeable TLDA (Taqman Low Density Array) Block (located at Mission Bay HD-235)
  • Also available for evaluation by Cancer Center members is an ABI 6100 semi-automated nucleic acid purification instrument that is capable of purifying 96 samples (up to 2 million cells/ well) from cells (from culture or sorting) to pure RNA in about 45 minutes.

Software

  • QBasePlus2 (Biogazelle) QPCR Analysis Software
  • Gene Codes Sequencher 4.7
  • ABI Primer Express 2.0 and 3.0
  • ABI SDS vs 2.1 and 2.3
  • ABI SeqScape 2.5
  • Softgenetics Mutation Surveyor
  • Sequenom Assay Design 3.1 (SNP Genotyping)
  • Sequenom Typer 3.4 and 4.0 (SNP Genotyping Analysis)
  • Sequenom EpiDesigner (Methylation Experiment Design)
  • Sequenom EpiTyper (Methylation Analysis)
  • Sequenom QGE (Quantitative Gene Expression Analysis)

Additional Resources
A searchable database of core facilities at all UCSF campus locations, provided by the Clinical and Translational Science Institute at UCSF, is available here.