About UCSF Search UCSF UCSF Medical Center

Reagents Available for Purchase

10X Taqman Buffer

Description
The Genome Core sells 10x TaqMan buffer containing the non-reactive, fluorescent ROX to normalize well-to-well variation.

Science
The principal behind using this buffer is using a passive reference dye in the PCR buffer to normalize the well-to-well variation. This variation can be a result of pipetting errors and/or variation in the optical properties of the 7900 instrument (e.g. fiber optics or optical caps/film). By spiking in a dual labeled probe that emits in the ~620nm range, this signal can be used to normalize the signal in the other wavelengths. The official ABI “buffer A” contains a 5’-FAM-TTTTTTTT-Rox-3’ probe at a final concentration of 60nM. The other buffer components have varied over time, but have basically been a standard PCR buffer without MgCl2. At the Genome Core we make it using ABI PCR Buffer II (P/N 4379878) with additional stabilizers.

Protocol
Available here.

Pricing
See pricing schedule here.

Additional Resources
A searchable database of core facilities at all UCSF campus locations, provided by the Clinical and Translational Science Institute at UCSF, is available here.