Biorepository and Tissue Biomarker Technology Core

Tissue Samples

• Acquisition in the OR & Clinics
• Multiple preservation methods with QA/QC
• Viable cell procurement
• Custodial storage/Distribution
• Retrospective FFPE Block Retrieval/Storage

Blood Samples

• Pickup from phlebotomy sites
• Whole Blood Processing –purified PBMC (QC), plasma, serum
• Custodial Storage/Distribution
• Secure Freezer Management

• Optimized processing of unfixed tissues for microtomy/biomarkers
• Embedding
• Cryo-microtomy
• FFPE microtomy
• RNase-free scrolls/sections
• Hematoxylin & Eosin staining
• Special histochemical stains
• Tissue microarray construction
• DNA/RNA extractions
• Histology instruction

• Immunohistochemistry/ Immunofluorescence -- single & multiplex staining
  • Ventana 5 marker IF/OPAL 7 marker IF
  • Chromogenic translucent cyes for IHC
• ACD RNAscope - RNA in situ
• Whole slide scanning – Ziess AxioScan Z1 & Vectra Quantitative Pathology Imaging System
• Object-based image analysis
• inForm software
• Definiens software