University of California San Francisco
Helen Diller Family Comprehensive Cancer Center

Biorepository and Tissue Biomarker Technology Core

Human Biospecimen Repository

Human Biospecimen Repository

Tissue Samples

  • Acquisition in the OR & Clinics
  • Multiple preservation methods with QA/QC
  • Viable cell procurement
  • Custodial storage/Distribution
  • Retrospective FFPE Block Retrieval/Storage

Blood Samples

  • Pickup from phlebotomy sites
  • Whole Blood Processing –purified PBMC (QC), plasma, serum
  • Custodial Storage/Distribution
  • Secure Freezer Management
Tissue Biospecimen Processing

Tissue Biospecimen Processing

  • Optimized processing of unfixed tissues for microtomy/biomarkers
  • Embedding
  • Cryo-microtomy
  • FFPE microtomy
  • RNase-free scrolls/sections
  • Hematoxylin & Eosin staining
  • Special histochemical stains
  • Tissue microarray construction
  • DNA/RNA extractions
  • Histology instruction
Tissue Biomarker Technology

Tissue Biomarker Technology

  • Immunohistochemistry/ Immunofluorescence -- single & multiplex staining
    • Ventana 5 marker IF/OPAL 7 marker IF
    • Chromogenic translucent cyes for IHC
  • ACD RNAscope - RNA in situ
  • Whole slide scanning – Ziess AxioScan Z1 & Vectra Quantitative Pathology Imaging System
  • Object-based image analysis
  • inForm software
  • Definiens software

Additional Resources

Histology Standard Operating Procedures