Recharge Rates
| Recharge Type | Internal Rate* | Affiliate Rate* | External Rate* |
| Construct design | $524.72 | $524.72 | $661.15 |
| Consultation | $314.83 | $314.83 | $396.69 |
| Cryopreservation (fertilized oocytes) | $2,096.95 | $2,096.95 | $2,642.16 |
| Cryopreservation (sperm) | $524.72 | $524.72 | $661.15 |
| Expansion of individual Clones of ES cells | $782.30 | $782.30 | $985.70 |
| Gene Targeting Screening Assay design | $333.91 | $333.91 | $420.73 |
| in vitro fertilization | $1,099.04 | $1,099.04 | $1,384.79 |
| Infection of fertilized oocytes with lentiviruses (B6D2) | $1,144.83 | $1,144.83 | $1,442.49 |
| Infection of fertilized oocytes with lentiviruses (C57BL/6) | $1,860.35 | $1,860.35 | $2,344.04 |
| Infection of fertilized oocytes with lentiviruses (FVB/N) | $1,144.83 | $1,144.83 | $1,442.49 |
| Infection of fertilized oocytes with lentiviruses (NOD) | $2,043.53 | $2,043.53 | $2,574.85 |
| Infection of fertilized oocytes with lentiviruses (NZB) | $3,291.40 | $3,291.40 | $4,147.16 |
| Infection of fertilized oocytes with lentiviruses (other) | $1,812.65 | $1,812.65 | $2,283.94 |
| Karyotype analysis of ES cell clones | $782.30 | $782.30 | $985.70 |
| Microinjection of DNA into FVB/N oocytes | $3,424.01 | $3,424.01 | $4,314.25 |
| Microinjection of DNA into B6D2 Embryos | $3,424.01 | $3,424.01 | $4,314.25 |
| Microinjection of DNA into NOD Embryos | $6,010.38 | $6,010.38 | $7,573.08 |
| Microinjection of DNA into NZB Embryos | $10,208.10 | $10,208.10 | $12,862.21 |
| Microinjection of DNA into C57BL/6 Embryos | $5,247.15 | $5,247.15 | $6,611.41 |
| Microinjection of Targeted 129 ES cells | $3,434.50 | $3,434.50 | $4,327.47 |
| Microinjection of Targeted C57BL/6 ES cells | $3,816.11 | $3,816.11 | $4,808.30 |
| Primary Gene Targeting Experiment (C57BL/6) | $4,102.32 | $4,102.32 | $5,168.92 |
| Primary Gene Targeting Experiment (FD-129 ES cells) | $3,816.11 | $3,816.11 | $4,808.30 |
| Primary Gene Targeting Experiment (FI-129 ES cells) | $3,434.50 | $3,434.50 | $4,327.47 |
| Recombineering (3 steps) | $2,480.47 | $2,480.47 | $3,125.39 |
| Recovery of mice from frozen embryos | $477.01 | $477.01 | $601.03 |
| Recovery of three 96-well plates of ES cells | $763.26 | $763.26 | $961.71 |
| Rederivation | $763.26 | $763.26 | $961.71 |
| Screening 96 ES cell clones (qPCR copy number assay) | $286.21 | $286.21 | $360.62 |
| Screening 96 ES cell clones (Conventional PCR) | $95.40 | $95.40 | $120.20 |
| Secondary Transfection of Gene Targeted Clones | $1,908.06 | $1,908.06 | $2,404.16 |
| Shipping mice | $477.01 | $477.01 | $601.03 |
* Internal users are funded by UCSF sources (or other UC campus sources) and provide a valid fund-DPA for payment. Affiliate users are funded by non-UC sources and pay by check, but the funding is provided by a recognized UCSF affiliate (SFGH, SFVAMC, NCIRE, Gallo, Gladstone, HHMI, or LBNL). External users are funded by non-UC sources and pay by check.
Acknowledgment
Publications generated from use of this facility should appropriately acknowledge the UCSF Comprehensive Cancer
Center Transgenic Core.
