Biorepository and Tissue Biomarker Technology
Human Biospecimen Repository
Tissue Samples
- Acquisition in the OR & Clinics
- Multiple preservation methods with QA/QC
- Viable cell procurement
- Custodial storage/Distribution
- Retrospective FFPE Block Retrieval/Storage
Blood Samples
- Pickup from phlebotomy sites
- Whole Blood Processing –purified PBMC (QC), plasma, serum
- Custodial Storage/Distribution
- Secure Freezer Management
Tissue Biospecimen Processing
- Optimized processing of unfixed tissues for microtomy/biomarkers
- Embedding
- Cryo-microtomy
- FFPE microtomy
- RNase-free scrolls/sections
- Hematoxylin & Eosin staining
- Special histochemical stains
- Tissue microarray construction
- DNA/RNA extractions
- Histology instruction
Tissue Biomarker Technology
- Immunohistochemistry/ Immunofluorescence -- single & multiplex staining
- Ventana 5 marker IF/OPAL 7 marker IF
- Chromogenic translucent cyes for IHC
- ACD RNAscope - RNA in situ
- Whole slide scanning – Ziess AxioScan Z1 & Vectra Quantitative Pathology Imaging System
- Object-based image analysis
- inForm software
- Definiens software
Additional Resources
Histology Standard Operating Procedures
