Charles S. Craik, PhD

Allegheny College, Meadville, PA, B.S., 1976, Chemistry
Columbia University, N.Y., NY, M.A., 1978, Chemistry
Columbia University, N.Y., NY, Ph.D., 1981, Chemistry

• 1981-85
  Postgraduate Research Biochemist, Department of Biochemistry & Biophysics, UCSF
• 1985-91
  Assistant Professor, Departments of Pharm. Chemistry and Biochemistry & Biophysics, UCSF
• 1991-1994
  Associate Professor, Departments of Pharm. Chemistry and Biochemistry & Biophysics, UCSF
• 1994-present
  Professor, Departments of Pharm. Chemistry, and Biochemistry & Biophysics, UCSF
• 1995-present
  Professor, Departments of Pharm. Chemistry, Pharmacology and Biochemistry & Biophysics, UCSF

• 1972-75
  National Honor Society; Alden Scholar
• 1974-75
  Doane Distinguished Scholar, Phi Beta Kappa
• 1975
  Richard Edwin Lee Scholar
• 1985-86
  Top 100 Innovators - Science Digest

• (195) A Multi-Enzyme Cascade of Hemoglobin Proteolysis in the Intestine of Blood-Feeding Hookworms. Williamson, A.L., Lecchi, P.X., Turk, B.E., Choe, Y., Hotez, P.J., McKerrow, J.H., Cantley, L.C., Sajid, M., Craik, C.S. and Loukas, A. J. Biol. Chem. 279(34), 35950-7 (2004)
• (196) Methyl Groups as Probes for Proteins and Complexes in In-Cell NMR Experiments. Serber, Z., Straub, W. Corsini, L.. Nomura, A.M., Shimba, Craik. C.S., N., Ortiz de Montellano, P. and Dotsch V. J. Amer. Chem. Soc. 126(22) 7119-25 (2004)
• (197) Optimization of (13) C direct detection NMR methods. Shimba, N. Kovack, H., Stern, A.S., Nomura, A.M., Shimada, I. Hoch, J.C., Craik, C.S., and Dotsch V. J. Biomol. NMR 30(2), 175-9. (2004)
• (198) Granzyme M is a Regulatory Protease that Inactivates Proteinase Inhibitor 9, an Endogenous Inhibitor of Granzyme B. Mahrus, S. Kisiel, W. and Craik, C.S. J. Biol. Chem. 279(52), 54275-82 (2004)
• (199) Trypsin. Halfon, S., Baird, T.T., and Craik, C.S. Handbook of Proteolytic Enzymes 2nd Ed. (2004)
• (200) Brachyurins. Tsu, C., and Craik, C.S. Handbook of Proteolytic Enzymes 2nd Ed. (2004)
• (201) Substrates of the Prostate-specific Serine Protease Prostate/KLK4 Defined by Positional-scanning Peptide Libraries. Matsumura, M. Bhatt, A.S., Andress, D., Clegg, N., Takayama, T.K. and Craik, C.S. Prostate 62(1), 1-13 (2005)
• (202) Papas Got a Brand New Tag: Advances in Identification of Proteases and their Substrates. Marnett, A.B. and Craik, C.S. Trends in Biotechnol. 23(2), 59-64 (2005)
• (203) Development of Alpha-Keto-based Inhibitors of Cruzain, a Cysteine Protease Implicated in Chagas Disease. Choe, Y., Brinen, L.S., Price, M.S., Engel, J.C., Lange, M. Grisostomi, C., Weston, S.G., Pallai, P.V., Cheng, H., Hardy, L.W., Hartsough, D.S., McMakin, M., Tilto, R.F. Baldino, C.M., and Craik, C.S. Bioorg. Med. Chem. 13(6), 2141-56 (2005)
• (204) Selective Chemical Functional Probes of Granzymes A and B Demonstrate Granzyme B is a Major Effector of Natural Killer Cell-Mediated Lysis of Target Cells. Mahrus, S. and Craik, C.S. Chem. Biol. 12(5), 567-77 (2005)
• (205) Adhesion Signaling By a Novel Mitotic Substrate of SRC Kinases. Bhatt, A., Erdjument-Bromage, H., Tempst, P., Craik, C.S., and Moasser, M. Oncogene, 24(34) 5333-43 (2005)
• (206) Hepatocyte Growth Factor is a Preferred In Vitro Substrate for Human Hepsin, a Membrane-anchored Serine Protease Implicated in Prostate and Ovarian Cancers. Herter, S., Piper, D.E., Aaron, W., Gabriele, T., Cutler, G., Cao, P., Bhatt, A.S., Choe, Y., Craik, C.S., Walker, N., Meininger, D., Hoey, T. and Austin, R.J. Biochem J. 390(1), 125-36 (2005)
• (207) Induced Structure: A Transitional Helix Switch that Regulates Enzyme Activity. *Nomura, A.M., *Marnett, A.B., Shimba, N., Doetch, V., and Craik, C.S. Nat. Struc. Mol. Bio. 12(11) 1019-20 (2005)
• (208) One Functional Switch Mediates Reversible and Irreversible Inactivation of a Herpesvirus Protease. Normura, A.M., Marnett, A.B., Shimba, N., Dtch, and Craik, C.S. Biochemistry 45(11), 3572-9 (2006)
• (209) Substrate Profiling of Cysteine Proteases Using a Combinatorial Peptide Library Identifies Functionally Unique Specificities. Choe, Y., Leonetti, F., Greenbaum, D.C., Lecaille, F., Bogyo, M., Brmme, D., Ellman, J.A., and Craik, C.S. J. Biol. Chem. 281(18), 12824-32
• (210) Conversion of Trypsin to a Functional Threonine Protease. Baird, T.T., Wright, W.D. and Craik, C.S. Protein Sci. 15(6), 1229-38
• (211) Specificity Profiling of Seven Human Tissue Kallikreins Reveals Individual Subsite Preferences. Debela, M., Magdolen, V., Schechter, N., Valachova, M., Lottspeich, F., Craik, C.S., Choe, Y., Bode, W., and Goettig, P. J. Biol. Chem. 2006 Sep 1; 281(35):25678-88
• (212) Plasminogen Activation Independent of uPA and tPA Maintains Wound Healing in Gene-Deficient Mice. Lund, L.R., Green, K.A., Stoop, A.A., Ploug, M., Almholt, K., Lilla, J., Nielsen, B.S., Christensen, I.J., Craik, C.S., Werb, Z., Dano, K. and Romer, J. EMBO J 2006 Jun 21; 25(12), 2686-97.
• (213) Granzyme B Proteolyses Receptors Important to Proliferation and Survival, Tipping the Balance Towards Apoptosis. Loeb, C.R.K., Harris, J. and Craik, C.S. J. Biol. Chem. 2006 Sep 22; 281(38):28326-35
• (214) Ecotin Modulates Thrombin Activity through Exosite-2 Interactions. Castro, H. C., Monteiro, R. Q., Assafim, M., Loureiro, N. I., Craik, C. S., Zingali, R. B. Int. J Biochem Cell Biol. 2006; 38(11), 1839-1900.
• (215) Expression and Characterization of Constitutively Active Human Caspase-14. Park, K., Kuechle, M. K. Choe, Y., Craik, C. S., Lawrence, O. T., Presland, R. B. Biochem Biophys Res Commun. 2006 Sep 8; 347(4), 941-8.
• (216) Isotope-Coded and Affinity-Tagged Cross-Linking (ICATXL): An Efficient Strategy to Probe Protein Interaction Surfaces. Chu, F., Mahrus, S., Craik, C. S., Burlingame, A. L. J Am Chem Soc. 2006 Aug 16; 128(32), 10362-3.