About UCSF Search UCSF UCSF Medical Center

David A. Agard, PhD


Professor, Departments of Biochemistry/Biophysics and Pharmaceutical Chemistry, UCSF; Scientific Director, Institute for Bioengineering, Biotechnology, and Quantitative Biomedical Research, UCSF/UC-Berkeley/UC-Santa Cruz


Phone: (415) 476-2521 (voice)
Box 2240, UCSF
San Francisco, CA 94143-2240

View on UCSF Profiles

Additional Websites


Yale University, New Haven, B.S., 1975, Molec. Biol. and Biophysics
California Institute of Technology, Pasadena, Ph.D., 1980, Biological Chemistry
University of California, San Francisco, Postdoctoral, 1980
MRC Laboratory, Cambridge, England, Postdoctoral, 1981-82

Professional Experience

  • 1975-1978
    National Science Foundation Predoctoral Fellow, California Institute of Technology.
  • 1980
    Postdoctoral Fellow with Dr. J.W. Sedat, Dept. of Biochemistry and Biophysics University of California, San Francisco (UCSF)
  • 1981
    Postdoctoral Fellow with Dr. R. Henderson, MRC Laboratory of Molecular Biology, Cambridge, England
  • 1983-1989
    Assistant Professor of Biochemistry & Biophysics, UCSF
  • 1986-1989
    Assistant Investigator in Structural Biology, Howard Hughes Medical Institute, UCSF
  • 1989-1992
    Associate Professor of Biochemistry & Biophysics, UCSF
  • 1986-1992
    Associate Investigator in Structural Biology, Howard Hughes Medical Institute, UCSF
  • 1992-present
    Professor of Biochemistry & Biophysics and of Pharmaceutical Chemistry, UCSF
  • 1993-present
    Investigator in Structural Biology, Howard Hughes Medical Institute UCSF
  • 1995-present
    Chair, Graduate Group in Biophysics, UCSF
  • 2001-2002
    Director, Institute for Bioengineering, Biotechnology, and Quantitative Biomedical Research, University of California, San Francisco, Berkeley, Santa Cruz
  • 2002-present
    Scientific Director, Institute for Bioengineering, Biotechnology, and Quantitative Biomedical Research, University of California, San Francisco, Berkeley, Santa Cruz

Honors & Awards

  • 1975-1978
    National Science Foundation Predoctoral Fellow
  • 1976
    Dreyfus Travelling Fellowship
  • 1980-1982
    Helen Hay Whitney Postdoctoral Fellow
  • 1983-1986
    Searle Scholar
  • 1983-1991
    Presidential Young Investigator's Award (NSF)
  • 1986
    Sidhu Award for Outstanding Contributions to Crystallography

Selected Publications

  1. Gordon DA, Smith ME, Wratschko M, Agard D, Holden L, Wilcox S, Lazorchak JM. A new approach for the laboratory culture of the fathead minnow, Pimephales promelas. Environ Toxicol Chem. 2014 Jan; 33(1):126-33.
    View on PubMed
  2. Miao Y, Wong CC, Mennella V, Michelot A, Agard DA, Holt LJ, Yates JR, Drubin DG. Cell-cycle regulation of formin-mediated actin cable assembly. Proc Natl Acad Sci U S A. 2013 Nov 19; 110(47):E4446-55.
    View on PubMed
  3. Arigovindan M, Fung JC, Elnatan D, Mennella V, Chan YH, Pollard M, Branlund E, Sedat JW, Agard DA. High-resolution restoration of 3D structures from widefield images with extreme low signal-to-noise-ratio. Proc Natl Acad Sci U S A. 2013 Oct 22; 110(43):17344-9.
    View on PubMed
  4. Heideman DA, Hesselink AT, van Kemenade FJ, Iftner T, Berkhof J, Topal F, Agard D, Meijer CJ, Snijders PJ. The Aptima HPV assay fulfills the cross-sectional clinical and reproducibility criteria of international guidelines for human papillomavirus test requirements for cervical screening. J Clin Microbiol. 2013 Nov; 51(11):3653-7.
    View on PubMed
  5. Li X, Zheng SQ, Egami K, Agard DA, Cheng Y. Influence of electron dose rate on electron counting images recorded with the K2 camera. J Struct Biol. 2013 Nov; 184(2):251-60.
    View on PubMed
  6. Li X, Mooney P, Zheng S, Booth CR, Braunfeld MB, Gubbens S, Agard DA, Cheng Y. Electron counting and beam-induced motion correction enable near-atomic-resolution single-particle cryo-EM. Nat Methods. 2013 Jun; 10(6):584-90.
    View on PubMed
  7. Genest O, Reidy M, Street TO, Hoskins JR, Camberg JL, Agard DA, Masison DC, Wickner S. Uncovering a region of heat shock protein 90 important for client binding in E. coli and chaperone function in yeast. Mol Cell. 2013 Feb 7; 49(3):464-73.
    View on PubMed
  8. Abrahamsson S, Chen J, Hajj B, Stallinga S, Katsov AY, Wisniewski J, Mizuguchi G, Soule P, Mueller F, Dugast Darzacq C, Darzacq X, Wu C, Bargmann CI, Agard DA, Dahan M, Gustafsson MG. Fast multicolor 3D imaging using aberration-corrected multifocus microscopy. Nat Methods. 2013 Jan; 10(1):60-3.
    View on PubMed
  9. Ozyamak E, Kollman J, Agard DA, Komeili A. The bacterial actin MamK: in vitro assembly behavior and filament architecture. J Biol Chem. 2013 Feb 8; 288(6):4265-77.
    View on PubMed
  10. Schneidman-Duhovny D, Rossi A, Avila-Sakar A, Kim SJ, Velázquez-Muriel J, Strop P, Liang H, Krukenberg KA, Liao M, Kim HM, Sobhanifar S, Dötsch V, Rajpal A, Pons J, Agard DA, Cheng Y, Sali A. A method for integrative structure determination of protein-protein complexes. Bioinformatics. 2012 Dec 15; 28(24):3282-9.
    View on PubMed
  11. Mennella V, Keszthelyi B, McDonald KL, Chhun B, Kan F, Rogers GC, Huang B, Agard DA. Subdiffraction-resolution fluorescence microscopy reveals a domain of the centrosome critical for pericentriolar material organization. Nat Cell Biol. 2012 Nov; 14(11):1159-68.
    View on PubMed
  12. Cunningham CN, Southworth DR, Krukenberg KA, Agard DA. The conserved arginine 380 of Hsp90 is not a catalytic residue, but stabilizes the closed conformation required for ATP hydrolysis. Protein Sci. 2012 Aug; 21(8):1162-71.
    View on PubMed
  13. Kraemer JA, Erb ML, Waddling CA, Montabana EA, Zehr EA, Wang H, Nguyen K, Pham DS, Agard DA, Pogliano J. A phage tubulin assembles dynamic filaments by an atypical mechanism to center viral DNA within the host cell. Cell. 2012 Jun 22; 149(7):1488-99.
    View on PubMed
  14. Kelch BA, Salimi NL, Agard DA. Functional modulation of a protein folding landscape via side-chain distortion. Proc Natl Acad Sci U S A. 2012 Jun 12; 109(24):9414-9.
    View on PubMed
  15. Shao L, Winoto L, Agard DA, Gustafsson MG, Sedat JW. Interferometer-based structured-illumination microscopy utilizing complementary phase relationship through constructive and destructive image detection by two cameras. J Microsc. 2012 Jun; 246(3):229-36.
    View on PubMed
  16. Arigovindan M, Sedat JW, Agard DA. Effect of depth dependent spherical aberrations in 3D structured illumination microscopy. Opt Express. 2012 Mar 12; 20(6):6527-41.
    View on PubMed
  17. Li S, Fernandez JJ, Marshall WF, Agard DA. Three-dimensional structure of basal body triplet revealed by electron cryo-tomography. EMBO J. 2012 Feb 1; 31(3):552-62.
    View on PubMed
  18. Street TO, Lavery LA, Verba KA, Lee CT, Mayer MP, Agard DA. Cross-monomer substrate contacts reposition the Hsp90 N-terminal domain and prime the chaperone activity. J Mol Biol. 2012 Jan 6; 415(1):3-15.
    View on PubMed
  19. Kollman JM, Merdes A, Mourey L, Agard DA. Microtubule nucleation by ?-tubulin complexes. Nat Rev Mol Cell Biol. 2011 Nov; 12(11):709-21.
    View on PubMed
  20. Alexander LD, Partridge JR, Agard DA, McAlpine SR. A small molecule that preferentially binds the closed conformation of Hsp90. Bioorg Med Chem Lett. 2011 Dec 1; 21(23):7068-71.
    View on PubMed

Go to UCSF Profiles, powered by CTSI