Danica Galonić Fujimori, PhD

Danica Galonić Fujimori, PhD

Assistant Professor of Cellular and Molecular Pharmacology and Pharmaceutical Chemistry, UCSF


Phone: (415) 514-0147
Box 2280, UCSF
San Francisco, CA 94143-2280

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Cancer Center Membership

Associate Member » Developmental Therapeutics

Research Summary

My lab is interested in elucidating how dynamic methylation of histone proteins within nucleosomes contributes to the regulation of transcription. In particular, we are investigating the function and regulation of Jumonji C domain-containing histone demethylases. We are approaching these challenges by combining chemical synthesis, protein biochemistry, mechanistic enzymology and cellular studies. In addition, my lab is interested in the catalysis and evolution of substrate specificity of a pair of related and mechanistically novel bacterial enzymes that methylate the peptidyltransferase center of the ribosome, causing resistance to five chemically distinct classes of antibiotics.


University of Belgrade, Belgrade, Serbia BSc 07/2000 Chemistry
University of Illinois, Urbana, IL PhD 05/2005 Chemistry
Harvard Medical School, Boston, MA Postdoc 06/2008 Biochemistry


Professional Experience

  • 7/05-6/08
    Postdoctoral Fellow, Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School
  • 7/08-pres.
    Assistant Professor, Department of Cellular and Molecular Pharmacology, Department of Pharmaceutical Chemistry, University of California San Francisco

Honors & Awards

  • 2000
    Serbian Chemical Society Outstanding Undergraduate Student Award
  • 2001
    University of Illinois Teachers Rated as Excellent
  • 2003
    Procter and Gamble Predoctoral Fellowship
  • 2004
    University of Illinois Pines Predoctoral Fellowship
  • 2005
    Damon Runyon Cancer Research Foundation Postdoctoral Fellowship
  • 2007
    NIH Pathway to Independence Award
  • 2009
    Kimmel Scholar Award
  • 2010
    V Foundation Scholar Award
  • 2011
    Basil O'Connor Starter Scholar Award, March of Dimes
  • 2011
    NSF CAREER Award
  • 2011
    Searle Scholar Award
  • 2014
    UCSF Excellence in Teaching Award

Selected Publications

  1. Fujimori DG. Radical SAM-mediated methylation reactions. Curr Opin Chem Biol. 2013 Aug; 17(4):597-604.
    View on PubMed
  2. Shiau C, Trnka MJ, Bozicevic A, Ortiz Torres I, Al-Sady B, Burlingame AL, Narlikar GJ, Fujimori DG. Reconstitution of nucleosome demethylation and catalytic properties of a Jumonji histone demethylase. Chem Biol. 2013 Apr 18; 20(4):494-9.
    View on PubMed
  3. Le DD, Cortesi AT, Myers SA, Burlingame AL, Fujimori DG. Site-specific and regiospecific installation of methylarginine analogues into recombinant histones and insights into effector protein binding. J Am Chem Soc. 2013 Feb 27; 135(8):2879-82.
    View on PubMed
  4. McCusker KP, Medzihradszky KF, Shiver AL, Nichols RJ, Yan F, Maltby DA, Gross CA, Fujimori DG. . Covalent Intermediate in the Catalytic Mechanism of the Radical S-Adenosyl-l-methionine Methyl Synthase RlmN Trapped by Mutagenesis. J Am Chem Soc. 2012; 134(43):18074-81.
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  5. Le DD, Fujimori DG. Protein and nucleic acid methylating enzymes: mechanisms and regulation. Curr Opin Chem Biol. 2012 Dec; 16(5-6):507-15.
    View on PubMed
  6. McCusker KP, Fujimori DG. The chemistry of peptidyltransferase center-targeted antibiotics: enzymatic resistance and approaches to countering resistance. ACS Chem Biol. 2012 Jan 20; 7(1):64-72.
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  7. Yan F, Fujimori DG. RNA methylation by radical SAM enzymes RlmN and Cfr proceeds via methylene transfer and hydride shift. Proc Natl Acad Sci U S A. 2011 Mar 8; 108(10):3930-4.
    View on PubMed
  8. Yan F, LaMarre JM, Röhrich R, Wiesner J, Jomaa H, Mankin AS, Fujimori DG. RlmN and Cfr are radical SAM enzymes involved in methylation of ribosomal RNA. J Am Chem Soc. 2010 Mar 24; 132(11):3953-64.
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  9. Wong C, Fujimori DG, Walsh CT, Drennan CL. Structural analysis of an open active site conformation of nonheme iron halogenase CytC3. J Am Chem Soc. 2009 Apr 8; 131(13):4872-9.
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  10. Fujimori DG. Hypoxia sensing goes gauche. Nat Chem Biol. 2009 Apr; 5(4):202-3.
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  11. Neumann CS, Fujimori DG, Walsh CT. Halogenation strategies in natural product biosynthesis. Chem Biol. 2008 Feb; 15(2):99-109.
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  12. Neidig ML, Brown CD, Light KM, Fujimori DG, Nolan EM, Price JC, Barr EW, Bollinger JM, Krebs C, Walsh CT, Solomon EI. CD and MCD of CytC3 and taurine dioxygenase: role of the facial triad in alpha-KG-dependent oxygenases. J Am Chem Soc. 2007 Nov 21; 129(46):14224-31.
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  13. Fujimori DG, Barr EW, Matthews ML, Koch GM, Yonce JR, Walsh CT, Bollinger JM, Krebs C, Riggs-Gelasco PJ. Spectroscopic evidence for a high-spin Br-Fe(IV)-oxo intermediate in the alpha-ketoglutarate-dependent halogenase CytC3 from Streptomyces. J Am Chem Soc. 2007 Nov 7; 129(44):13408-9.
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  14. Fujimori DG, Hrvatin S, Neumann CS, Strieker M, Marahiel MA, Walsh CT. Cloning and characterization of the biosynthetic gene cluster for kutznerides. Proc Natl Acad Sci U S A. 2007 Oct 16; 104(42):16498-503.
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  15. Fujimori DG, Walsh CT. What's new in enzymatic halogenations. Curr Opin Chem Biol. 2007 Oct; 11(5):553-60.
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  16. Krebs C, Galonic Fujimori D, Walsh CT, Bollinger JM. Non-heme Fe(IV)-oxo intermediates. Acc Chem Res. 2007 Jul; 40(7):484-92.
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  17. Galonic DP, Gin DY. Chemical glycosylation in the synthesis of glycoconjugate antitumour vaccines. Nature. 2007 Apr 26; 446(7139):1000-7.
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  18. Kelly WL, Boyne MT, Yeh E, Vosburg DA, Galonic DP, Kelleher NL, Walsh CT. Characterization of the aminocarboxycyclopropane-forming enzyme CmaC. Biochemistry. 2007 Jan 16; 46(2):359-68.
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  19. Galonic DP, Barr EW, Walsh CT, Bollinger JM, Krebs C. Two interconverting Fe(IV) intermediates in aliphatic chlorination by the halogenase CytC3. Nat Chem Biol. 2007 Feb; 3(2):113-6.
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  20. Ueki M, Galonic DP, Vaillancourt FH, Garneau-Tsodikova S, Yeh E, Vosburg DA, Schroeder FC, Osada H, Walsh CT. Enzymatic generation of the antimetabolite gamma,gamma-dichloroaminobutyrate by NRPS and mononuclear iron halogenase action in a streptomycete. Chem Biol. 2006 Nov; 13(11):1183-91.
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