Associate Professor, Department of Medicine, UCSF
Research SummaryI have a long history of vascular research and am actively studying the impairment of rat vascular function (arterial flow-mediated dilation, FMD) by tobacco and marijuana secondhand smoke. My lab develop¬ed the main approach being used for these studies (FMD measurements of endothelial function in living rats). We have published one paper introducing this approach and using it to explore aspects of vascular function, plus a paper about its use to explore how endothelial function is influenced by pharmacological manipulation of intracellular nitric oxide storage pools, and a paper published recently about impairment of FMD in rat by expo¬s¬ure to as little as one minute of tobac¬co sidestream smoke. My laboratory has also participated in studies of human exposure to second¬hand smoke and its effects on FMD and circulating angiogenic cell func¬tion. The combina¬tion of the my lab’s productivity in secondhand smoke research, its development of the technique to measure rat endothe¬lial function, and a long prior history of vascular biology research, make me uniquely well-positioned to study impairment of vascular function by smoke exposure. Furthermore, since 2005, I have previously been the PI on an R01, two R21s, an R03, a P50 subproject, and a phase I SBIR that are completed; and currently I am the PI of a five-year R01 focused on how exposure to diverse tobacco products affects vascular function and endothelial production of nitric oxide. This demonstrates a consistent pattern of productivity in NIH-funded research. My active R01 study and my past P50 subproj¬ect are align¬ed with the activities of the Center for Tobacco Control Research and Education (CTCRE), and I have been a member of the UCSF Tobacco Center of Regulatory Science (TCORS) since its inception.
University of California, Berkeley, CA, B.A., 1981-1985, Molecular Biology
Stanford University, Stanford, CA, Ph.D., 1985-1991, Biological Sciences
Stanford University, Stanford, CA, (postdoctoral), 1991-1993, Cell Biology/Biochemistry
Stanford University, Stanford, CA, (postdoctoral), 1993-1997, Molecular Pharmacology
Research Scientist, Department of Molecular Pharmacology, Stanford University
Senior Research Scientist, Department of Molecular Pharmacology, Stanford University
Assistant Professor, Division of Cardiology, University of California, San Francisco
Associate Professor, Division of Cardiology, University of California, San Francisco
Investigator, Cardiovascular Research Institute, University of California, San Francisco
Honors & Awards
President, Molecular Biology Undergraduate Society, UC Berkeley
Selected graduation speaker, Department of Molecular Biology, UC Berkeley
Graduated with distinction, UC Berkeley
Award for Exceptional Contributions in Teaching, Department of Biological Sciences, Stanford University
Elected Graduate Student Representative, Dept. of Biological Sciences, Stanford University
4th place winner (out of >2000), Nikon Small World Competition for microscopy
- Springer, M.L. and Yanofsky, C. (1989). A morphological and genetic analysis of conidiophore development in Neurospora crassa. Genes Dev. 3:559-571.
- Springer, M.L. (1989). An effective procedure for the preparation of Neurospora crassa conidiophores for scanning electron microscopy. Fungal Genet. Newsl. 36:78.
- Springer, M.L. (1991). Tergitol-induced colonial growth without inhibition of conidiation. Fungal Genet. Newsl. 38:92.
- Springer, M.L., Hager, K.M., Garrett-Engele, C., and Yanofsky, C. (1992). Timing of synthesis and cellular localization of two conidiation-specific proteins of Neurospora crassa. Dev. Biol. 152:255-262.
- Springer, M.L. and Yanofsky, C. (1992). Expression of con genes along the three sporulation pathways of Neurospora crassa. Genes Dev. 6:1052-1057.
- Springer, M.L. (1993). Genetic control of fungal differentiation: The three sporulation pathways of Neurospora crassa. BioEssays 15:365-374.
- Springer, M.L., Patterson, B., and Spudich, J.A. (1994). Stage-specific requirement for myosin II during Dictyostelium development. Development 120:2651-2660.
- Blau, H.M. and Springer, M.L. (1995). Gene therapy--A novel form of drug delivery. N. Engl. J. Med. 333:1204-1207.
- Blau, H.M. and Springer, M.L. (1995). Muscle-mediated gene therapy. N. Engl. J. Med. 333:1554-1556.
- Springer, M.L. and Blau, H.M. (1997). High-efficiency retroviral infection of primary myoblasts. Som. Cell Mol. Genet. 23:203-209.
- Kang, S.-M., Hofmann, A., Le, D.L., Springer, M.L., Stock, P.G., and Blau, H.M. (1997). Immune response and myoblasts that express Fas ligand. Science 278:1322-1324.
- Springer, M.L., Kraft, P.E., and Blau, H.M. (1998). Inhibition of solid tumor growth by Fas ligand-expressing myoblasts. Som. Cell Mol. Genet. 24:281-289.
- Springer, M.L., Chen, A.S., Kraft, P.E., Bednarski, M., and Blau, H.M. (1998). VEGF gene delivery to muscle: Potential role for vasculogenesis. Mol. Cell 2:549-558.
- Abendroth, A., Slobedman, B., Springer, M.L., Blau, H.M., and Arvin, A.M. (1999). Analysis of immune responses to varicella zoster viral proteins induced by DNA vaccination. Antiviral Res. 44: 179-192.
- Springer, M.L., Ip, T.K., and Blau, H.M. (2000). Angiogenesis monitored by perfusion with a space-filling microbead suspension. Mol. Therapy 1: 82-87.
- Springer, M.L., Hortelano, G., Bouley, D., Wong, J., Kraft, P.E., and Blau, H.M. (2000). Induction of angiogenesis by implantation of encapsulated primary myoblasts expressing vascular endothelial growth factor. J. Gene Med. 2:279-288.
- Lee, R.J., Springer, M.L., Blanco-Bose, W.E., Shaw, R., Ursell, P.C., and Blau, H.M. (2000). VEGF gene delivery to myocardium: Deleterious effects of unregulated expression. Circulation 102:898-901.
- Springer, M.L., Ozawa, C.R., and Blau, H.M. (2002). Transient production of -smooth muscle actin by skeletal myoblasts during differentiation in culture and following intramuscular implantation. Cell Motil. Cytoskeleton 51:177-186.
- Munz, B., Hildt, E., Springer, M.L., and Blau, H.M. (2002). RIP2, a checkpoint in myogenic differentiation. Mol. Cell Biol. 22:5879-5886.
- Springer, M.L., Ozawa, C.R., Banfi, A., Kraft, P.E., Ip, T.K., Brazelton, T.R., and Blau, H.M. (2003). Localized arteriole formation adjacent to sites of implantation of myoblasts expressing VEGF. Mol. Ther., 7:441-449.
- Ozawa, C.R., Banfi, A., Glazer, N., Thurston, G., Springer, M.L., Kraft, P.E., McDonald, D.M., and Blau, H.M. (2004). Microenvironmental VEGF concentration, not total dose, determines a threshold between normal and aberrant angiogenesis. J. Clin. Invest. 113: 516-527.
- Springer, M.L., Sievers, R.E., Viswanathan, M., Yee, M.S., Foster, E., Grossman, W., and Yeghiazarians, Y. (2005). Closed-chest cell injections into mouse myocardium guided by high-resolution echocardiography. Amer. J. Physiol. Heart Circ. Physiol., 289:H1307-H1314.
- Kamba, T., Tam, B.Y.Y., Hashizume, H., Haskell, A., Sennino, B., Mancuso, M.R., Norberg, S.M., O'Brien, S.M., Davis, R.B., Thurston, G., Joho, S., Springer, M.L., Kuo, C.J., and McDonald, D.M. (2006). VEGF-dependent plasticity of fenestrated capillaries in the normal adult microvasculature. Amer. J. Physiol. Heart Circ. Physiol. 290:H560-H576.
- Springer, M.L. (2006). A Balancing Act: Therapeutic Approaches to the Modulation of Angiogenesis. Current Opinion in Investigational Drugs 7:243-250.
- von Degenfeld, G., Banfi, A., Springer, M.L., Jacobi, J., Ozawa, C.R., Merchant, M.J., Cooke, J.P., and Blau, H.M. (2006). Microenvironmental concentration of VEGF is critical to stable and functional vessel growth in ischemia. FASEB J., 20:2657-2659.
- Zhang, Y., Takagawa, J., Sievers, R.E., Khan, M.F., Viswanathan, M.N., Springer, M.L., Foster, E., and Yeghiazarians, Y. (2007). Validation of the wall motion score and myocardial performance indices as novel techniques to assess left ventricular function in mice post myocardial infarction. Amer. J. Physiol. Heart Circ. Physiol., 292:H1187-H1192.
- Takagawa, J., Zhang, Y., Sievers, R.E., Kapasi, N.K., Wong, M.L., Wang, Y., Yeghiazarians, Y., Lee, R.J., Grossman, W., and Springer, M.L. (2007). Myocardial infarct size measurement in the mouse chronic infarction model: Comparison of area- and length-based approaches. J. Appl. Physiol. 102:2104-2111.
- Springer, M.L., Banfi, A., Ye, J., von Degenfeld, G., Kraft, P.E., Saini, S.A., Kapasi, N.K., and Blau, H.M. (2007). Localization of vascular response to VEGF is not dependent on heparin binding. FASEB J. 21:2074-2085.
- Heiss, C., Sievers, R.E., Amabile, N., Momma, T.Y., Chen, Q., Natarajan, S., Yeghiazarians, Y., and Springer, M.L. (2008). In vivo measurement of flow-mediated vasodilation in living rats using high resolution ultrasound. Amer. J. Physiol. Heart Circ. Physiol. 294:H1086-H1093.
- Heiss, C., Wong, M.L., Block, V.I., Lao, D., Real, W.M., Yeghiazarians, Y., Lee, R.J., and Springer, M.L. (2008). Pleiotrophin induces nitric oxide dependent migration of endothelial progenitor cells. J. Cell. Physiol. 215:366-373.
- Heiss, C., Amabile, N., Lee, A.C., Real, W.M., Schick, S., Lao, D., Wong, M.L., Jahn, S., Angeli, F.S., Minasi, P., Springer, M.L., Hammond, S.K., Glantz, S.A., Grossman, W., Balmes, J.R., and Yeghiazarians, Y. (2008). Second hand smoke causes acute vascular injury, and mobilization of dysfunctional endothelial progenitor cells. J. Am. Coll. Cardiol. 51:1760-1771.
- Li, X., Tjwa, M., Van Hove, I., Enholm, B., Neven E., Paavonen, K., Jeltsch, M., Diez Juan, T., Sievers, R.E., Chorianopoulos, E., Wada, H., Vanwildemeersch, M., Noel, A., Foidart, J.-M., Springer, M.L., von Degenfeld, G., Dewerchin, M., Blau, H.M., Alitalo, K., Eriksson, U., Carmeliet, P. and Moons, L. (2008). Re-evaluation of the role of VEGF-B suggests a restricted role in the revascularization of the ischemic myocardium. Arterioscler. Thromb. Vasc. Biol. 28:1614-1620.
- Yeghiazarians, Y., Zhang, Y., Prasad, M., Shih, H., Saini, S.A., Takagawa, J., Sievers, R.E., Wong, M.L., Kapasi, N.K., Mirsky, R., Koskenvuo, J., Minasi, P., Ye, J., Viswanathan, M.N., Angeli, F.S., Boyle A.J., Springer, M.L., and Grossman, W. (2009). Injection of bone marrow cell extract into infarcted hearts results in functional improvement comparable to intact cell therapy. Mol. Ther. 17:1250-1256.