University of California San Francisco
Helen Diller Family Comprehensive Cancer Center
Sharof M. Tugizov, DVM, PhD, DSc

Sharof M. Tugizov, DVM, PhD, DSc

Professor, Department of Medicine, UCSF

Cancer Center Program Memberships

Cancer Immunology

Research Summary

My research focus is investigation of the interactions of human viruses, including human cytomegalovirus (HCMV), Epstein–Barr virus (EBV), Herpes simplex virus (HSV), human immunodeficiency virus (HIV), and human papillomavirus (HPV), with monostratified and multistratified oral epithelia. We have made significant contributions to current knowledge about entry, transcytosis, intracellular transport, egress, and cell-to-cell transmission of these viruses in oral epithelial cells by establishing and using polarized epithelial cells, as well as ex vivo oral mucosal tissue explants. Currently, we are investigating the potential interactions of HIV with other viral pathogens, including human papillomavirus HPV. The incidence of HPV-associated oral cancer in human immunodeficiency virus (HIV)-positive individuals is substantially higher than in HIV-uninfected individuals. Our data show that oral epithelial biopsies from HIV-infected individuals and oral keratinocytes grown in tissue culture from HIV-infected individuals have multiple changes consistent with epithelial-mesenchymal transition (EMT), a multistep epigenetic process characterized by loss of cell adhesion and increased proliferation and mobility of epithelial cells. EMT is important in cell differentiation during embryogenesis. However, it is also plays a critical role in progression of neoplastic processes. We expect that our research may led to better understanding the role of HIV-associated EMT in development of HPV-associated neoplasia.

Education

Institute of Agriculture, School of Veterinary Medicine, Samarkand, Uzbekistan (former Soviet Union), DVM 1981, Veterinary Medicine
Institute of Experimental Veterinary Medicine and Ivanosky Institute of Virology, Moscow, Russia, PhD, 1985, Virology, Cell Biology
Ivanosky Institute of Virology, Russian Academy of Medical Sciences, Moscow, Russia, DSc, 1996, Medical Virology Infectious Disease


Professional Experience

  • 1985-88
    Junior Research Scientist, Dept. of Virus Physiology, Ivanovsky Institute of Virology, Academy of Medical Sciences, Moscow, USSR
  • 1988-91
    Senior Research Scientist, Dept. of Molecular Biology, Ivanovsky Institute of Virology, Academy of Medical Sciences, Moscow, USSR
  • 1991-94
    Visiting Scientist, Department of Stomatology, School of Dentistry, University of California, San Francisco, California
  • 1994-99
    Researcher in Virology and Molecular Biology, Department of Stomatology, School of Dentistry, University of California, San Francisco, California
  • 1999-2005
    Assistant Adjunct Professor, Department of Medicine, School of Medicine, University of California, San Francisco, California
  • 2005-11
    Associate Professor, Department of Medicine, School of Medicine, University of California, San Francisco, California
  • 2011-current
    Professor, Department of Medicine, School of Medicine, University of California, San Francisco, California

Honors & Awards

  • 2001 Hellman Fellow
  • Ad hoc reviewer for NIH, USA, 2006
  • Ad hoc reviewer for NIH, USA, 2009
  • Ad hoc reviewer for Cancer Research Institute, United Kingdom, 2002
  • Ad hoc reviewer for Cancer Research Institute, United Kingdom, 2006
  • Ad hoc reviewer for Cancer Research Institute, United Kingdom, 2009
  • Ad hoc reviewer for Hellman Family Foundation, 2010
  • Ad hoc referee for Journal of Virology, Journal of Dermatology, In vitro, Journal of Oral Pathology and Medicine, Voprosi Virusologii (in Russian), British Journal of Dermatology, Plos Pathogens, Virology, Journal of Dental Research, Chemotherapy, AIDS, Viral Immunology
  • Member of Editorial Board, Virology& Mycology

Selected Publications

  1. Wechsler EI, Tugizov S, Herrera R, Costa MD, Palefsky JM. Erratum: E5 can be expressed in anal cancer and leads to epidermal growth factor receptor-induced invasion in a human papillomavirus 16-transformed anal epithelial cell line. J Gen Virol. 2018 Jul; 99(7):948.
    View on PubMed
  2. Sufiawati I, Tugizov SM. HIV-induced matrix metalloproteinase-9 activation through mitogen-activated protein kinase signalling promotes HSV-1 cell-to-cell spread in oral epithelial cells. J Gen Virol. 2018 May 18.
    View on PubMed
  3. Wechsler EI, Tugizov S, Herrera R, Da Costa M, Palefsky JM. E5 can be expressed in anal cancer and leads to epidermal growth factor receptor-induced invasion in a human papillomavirus 16-transformed anal epithelial cell line. J Gen Virol. 2018 Apr 06.
    View on PubMed
  4. Yasen A, Herrera R, Rosbe K, Lien K, Tugizov SM. HIV internalization into oral and genital epithelial cells by endocytosis and macropinocytosis leads to viral sequestration in the vesicles. Virology. 2018 Feb; 515:92-107.
    View on PubMed
  5. Yasen A, Herrera R, Rosbe K, Lien K, Tugizov SM. Release of HIV-1 sequestered in the vesicles of oral and genital mucosal epithelial cells by epithelial-lymphocyte interaction. PLoS Pathog. 2017 Feb; 13(2):e1006247.
    View on PubMed
  6. Haga T, Efird JT, Tugizov S, Palefsky JM. Increased TNF-alpha and sTNFR2 levels are associated with high-grade anal squamous intraepithelial lesions in HIV-positive patients with low CD4 level. Papillomavirus Res. 2017 Jun; 3:1-6.
    View on PubMed
  7. Tugizov S. Human immunodeficiency virus-associated disruption of mucosal barriers and its role in HIV transmission and pathogenesis of HIV/AIDS disease. Tissue Barriers. 2016 Jul-Sep; 4(3):e1159276.
    View on PubMed
  8. Herrera R, Morris M, Rosbe K, Feng Z, Weinberg A, Tugizov S. Human beta-defensins 2 and -3 cointernalize with human immunodeficiency virus via heparan sulfate proteoglycans and reduce infectivity of intracellular virions in tonsil epithelial cells. Virology. 2016 Jan; 487:172-87.
    View on PubMed
  9. Sufiawati I, Tugizov SM. HIV-associated disruption of tight and adherens junctions of oral epithelial cells facilitates HSV-1 infection and spread. PLoS One. 2014; 9(2):e88803.
    View on PubMed
  10. Tugizov SM, Herrera R, Chin-Hong P, Veluppillai P, Greenspan D, Michael Berry J, Pilcher CD, Shiboski CH, Jay N, Rubin M, Chein A, Palefsky JM. HIV-associated disruption of mucosal epithelium facilitates paracellular penetration by human papillomavirus. Virology. 2013 Nov; 446(1-2):378-88.
    View on PubMed
  11. Tugizov SM, Herrera R, Palefsky JM. Epstein-Barr virus transcytosis through polarized oral epithelial cells. J Virol. 2013 Jul; 87(14):8179-94.
    View on PubMed
  12. Tugizov SM, Herrera R, Veluppillai P, Greenspan D, Soros V, Greene WC, Levy JA, Palefsky JM. Differential transmission of HIV traversing fetal oral/intestinal epithelia and adult oral epithelia. J Virol. 2012 Mar; 86(5):2556-70.
    View on PubMed
  13. Challacombe SJ, Fidel PL, Tugizov S, Tao L, Wahl SM. HIV infection and specific mucosal immunity: workshop 4B. Adv Dent Res. 2011 Apr; 23(1):142-51.
    View on PubMed
  14. Weinberg A, Naglik JR, Kohli A, Tugizov SM, Fidel PL, Liu Y, Herzberg M. Innate immunity including epithelial and nonspecific host factors: workshop 1B. Adv Dent Res. 2011 Apr; 23(1):122-9.
    View on PubMed
  15. Tugizov SM, Webster-Cyriaque JY, Syrianen S, Chattopadyay A, Sroussi H, Zhang L, Kaushal A. Mechanisms of viral infections associated with HIV: workshop 2B. Adv Dent Res. 2011 Apr; 23(1):130-6.
    View on PubMed
  16. Tugizov SM, Herrera R, Veluppillai P, Greenspan D, Soros V, Greene WC, Levy JA, Palefsky JM. HIV is inactivated after transepithelial migration via adult oral epithelial cells but not fetal epithelial cells. Virology. 2011 Jan 20; 409(2):211-22.
    View on PubMed
  17. Dorner M, Zucol F, Alessi D, Haerle SK, Bossart W, Weber M, Byland R, Bernasconi M, Berger C, Tugizov S, Speck RF, Nadal D. beta1 integrin expression increases susceptibility of memory B cells to Epstein-Barr virus infection. J Virol. 2010 Jul; 84(13):6667-77.
    View on PubMed
  18. Xiao J, Palefsky JM, Herrera R, Sunshine C, Tugizov SM. EBV-positive human sera contain antibodies against the EBV BMRF-2 protein. Virology. 2009 Oct 10; 393(1):151-9.
    View on PubMed
  19. Xiao J, Palefsky JM, Herrera R, Berline J, Tugizov SM. EBV BMRF-2 facilitates cell-to-cell spread of virus within polarized oral epithelial cells. Virology. 2009 Jun 05; 388(2):335-43.
    View on PubMed
  20. Haga T, Uchide N, Tugizov S, Palefsky JM. Role of E-cadherin in the induction of apoptosis of HPV16-positive CaSki cervical cancer cells during multicellular tumor spheroid formation. Apoptosis. 2008 Jan; 13(1):97-108.
    View on PubMed

Go to UCSF Profiles, powered by CTSI