David P. Toczyski, PhD

David P. Toczyski, PhD

Professor, Department of Biochemistry and Biophysics, UCSF


Phone: (415) 502-1301 (voice)
Box 0128, UCSF
San Francisco, CA 94143-0128

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Cancer Center Membership

Program Member » Non-aligned


State University of New York/Buffalo, B.S., 1987, Biology
Yale University, M.Phil., 1990, Biophysics & Biochem
Yale University, Ph.D., 1992, Biophysics & Biochem

Professional Experience

  • 1984-1986
    State University New York - Buffalo, Undergrad Research
  • 1987-1992
    Yale University, Graduate Student
  • 1993-1998
    University of Washington/FHCRC, Postdoctoral Associate
  • 1998-2005
    UCSF Biochemistry & Biophysics/CRI, Assistant Professor
  • 2005-2010
    UCSF Biochemistry & Biophysics/CRI, Associate Professor
  • 2010-present
    UCSF Biochemistry & Biophysics/CRI,  Professor

Honors & Awards

  • 1993-1996
    Jane Coffin Childs Fellow
  • 2003-2009
    Leukemia and Lymphoma Society Scholar
  • 2005-present
    NDT study section, permanent member
  • 2009
    Keynote address, NIH Cell Cycle meeting

Selected Publications

  1. Isolation of ubiquitinated substrates by tandem affinity purification of E3 ligase-polyubiquitin-binding domain fusions (ligase traps). Nat Protoc. 2016 Feb; 11(2):291-301.
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  2. Parallel Parkin: Cdc20 Takes a New Partner. Mol Cell. 2015 Oct 1; 60(1):3-4.
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  3. Prb1 Protease Activity Is Required for Its Recognition by the F-Box Protein Saf1. Biochemistry. 2015 Jul 28; 54(29):4423-6.
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  4. DNA Damage Regulates Translation through ß-TRCP Targeting of CReP. PLoS Genet. 2015 Jun; 11(6):e1005292.
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  5. Ndd1 Turnover by SCFGrr1 Is Inhibited by the DNA Damage Checkpoint in Saccharomyces cerevisiae. PLoS Genet. 2015 Apr; 11(4):e1005162.
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  6. Acetylome profiling reveals overlap in the regulation of diverse processes by sirtuins, gcn5, and esa1. Mol Cell Proteomics. 2015 Jan; 14(1):162-76.
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  7. Polymerase stalling during replication, transcription and translation. Curr Biol. 2014 May 19; 24(10):R445-52.
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  8. Hst3 is turned over by a replication stress-responsive SCF(Cdc4) phospho-degron. Proc Natl Acad Sci U S A. 2014 Apr 22; 111(16):5962-7.
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  9. Ubiquitin ligase trapping identifies an SCF(Saf1) pathway targeting unprocessed vacuolar/lysosomal proteins. Mol Cell. 2014 Jan 9; 53(1):148-61.
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  10. Rad53 downregulates mitotic gene transcription by inhibiting the transcriptional activator Ndd1. Mol Cell Biol. 2014 Feb; 34(4):725-38.
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  11. Gcn5 and sirtuins regulate acetylation of the ribosomal protein transcription factor Ifh1. Curr Biol. 2013 Sep 9; 23(17):1638-48.
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  12. F-box protein specificity for g1 cyclins is dictated by subcellular localization. PLoS Genet. 2012; 8(7):e1002851.
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  13. Ubiquitin ligases: Taming the APC. Nat Chem Biol. 2012 Apr; 8(4):323-4.
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  14. Keeping it together in times of stress: checkpoint function at stalled replication forks. Mol Cell. 2012 Mar 9; 45(5):585-6.
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  15. Colocalization of Mec1 and Mrc1 is sufficient for Rad53 phosphorylation in vivo. Mol Biol Cell. 2012 Mar; 23(6):1058-67.
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  16. Ubiquitination of Cdc20 by the APC occurs through an intramolecular mechanism. Curr Biol. 2011 Nov 22; 21(22):1870-7.
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  17. Structural biology: a new look for the APC. Nature. 2011 Feb 10; 470(7333):182-3.
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  18. Cross-species chemogenomic profiling reveals evolutionarily conserved drug mode of action. Mol Syst Biol. 2010 Dec 21; 6:451.
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  19. Cdc5 blocks in vivo Rad53 activity, but not in situ activity (ISA). Cell Cycle. 2010 Nov 1; 9(21):4266-8.
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  20. Damage-induced phosphorylation of Sld3 is important to block late origin firing. Nature. 2010 Sep 23; 467(7314):479-83.
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