University of California San Francisco
Helen Diller Family Comprehensive Cancer Center

Flow Cytometry & Cell Sorting

Flow Cytometry & Cell Sorting Instruments and Services

The LCA provides access and training on two FACS Calibur benchtop cytometers, one FACS Canto II multi-laser digital cytometer, one FACS Aria II cell sorter, one FACS Aria III cell sorter, and one Sony SH800 cell sorter. These systems are primarily used for the rapid analysis and sorting of single-cell suspensions. To analyze the light scatter and fluorescence characteristics of a cell population, one of the benchtop machines is appropriate. For more complex assays requiring more than four differentiating markers, then the high-end multi-laser Canto II system would work well. To rapidly retrieve or sort a particular sub-group of cells from a mixed sample, then a cell sorter would be required.

Instrument Operation/Training Rates:
Internal Rate: $94.64/hour
External Rate: $166.94/hour

For operator and technical assistance and core staff time, please contact Sarah Elmes at Sarah.Elmes@ucsf.edu


FACS Aria II

FACS Aria IIThe FACS Aria II is a high-speed digital sorter that incorporates a fixed-alignment cuvette flow cell. Fiber optics direct and focus five lasers (360nm, 405nm, 488nm, 561nm, and 633nm) onto the alignment prisms and then focuses on the cuvette flow cell. The flow cell allows for improved light excitation and collection optics. The collection optics consist of octagon- and trigon-shaped collection devices, and a total of up to 18 independent signals can be acquired at one time on a single-cell basis, followed by purification of cell subsets by sorting into tubes or multiwell formats.

Location: Room S252, Genentech Hall, Mission Bay Campus

Contact: For operator and technical assistance on this cell sorter, please contact Sarah Elmes at Sarah.Elmes@ucsf.edu.

Rates:
Self-Operated: $56.55/hour (internal rate) or $99.76/hour (external rate)
With Operator Assistance: $151.19/hour (internal rate) or $266.70 (external rate)

FACS Aria II


FACS Aria III

FACS Aria IIThe FACS Aria III is a high-speed digital sorter that incorporates a fixed-alignment cuvette flow cell. Fiber optics direct and focus four lasers (407nm, 488nm, 561nm, and 633nm) onto the alignment prisms and then focuses on the cuvette flow cell. The flow cell allows for improved light excitation and collection optics. The collection optics consist of octagon- and trigon-shaped collection devices, and a total of up to 18 independent signals can be acquired at one time on a single-cell basis, followed by purification of cell subsets by sorting into tubes or multiwell formats.

Location: Room HD335, Helen Diller Building, Mission Bay Campus

Contact: For operator and technical assistance on this cell sorter, please contact Sarah Elmes at Sarah.Elmes@ucsf.edu.

Rates:
Self-Operated: $56.55/hour (internal rate) or $99.76/hour (external rate)
With Operator Assistance: $151.19/hour (internal rate) or $266.70 (external rate)

FACS Aria III


FACS Canto II

FACSCanto II from Becton Dickinson is designed for advanced analytical research applications using digital signal processing and flow cell optics, similar to the Aria II systems. The Canto II is equipped with the latest technical capabilities - high-speed electronics, multiple laser excitations across a wide spectral range (from 405 up to 640nm) and up to eighteen PMT detectors, and HTS 96 well plate reader.

Location: Room 512, Rock Hall, Mission Bay Campus

Contact: For operator and technical assistance on this flow cytometer, please contact Jane Gordon at Jane.Gordon@ucsf.edu.

Rates:
Self-Operated: $48.61/hour (internal rate) or $85.75/hour (external rate)
With Operator Assistance: $143.25/hour (internal rate) or $252.69 (external rate)

FACS Canto II


FACS Calibur

FACS CaliburFACS Calibur is a benchtop flow cytometer designed for applications ranging from routine clinical to basic research. The system analyzes cells as they pass one at a time through two focused laser beams, a 488nm argon-ion laser and a 635nm red-diode laser. The FACS Calibur system is simpler than the Canto II measuring forward light scatter (FCS), side scatter (SSC), and four fluorescence parameters as well as the pulse area and width of any fluorescence parameter and time. 

Location: 
Room S301A, Genentech Hall, Mission Bay Campus
Room S171, Cancer Research Building, Mt Zion Campus

Contact:
Mission Bay: Sarah.Elmes@ucsf.edu
Mt Zion: Jane.Gordon@ucsf.edu

Rates:
Self-Operated: $48.61/hour (internal rate) or $85.75/hour (external rate)
With Operator Assistance: $143.25/hour (internal rate) or $252.69 (external rate)

FACS Calibur


BD FACSVerse

BD FACSVerseThe BD FACSVerse flow cytometer is designed to support cell analysis for research applications using up to 10 parameters.  It uses free-space lasers to concentrate intensity at the flow cell.  It is designed to minimize light loss and maximize resolution for multicolor applications.  It is designed for up to three lasers and has fixed alignment. 

Location: HD254, Helen Diller Building, Mission Bay Campus

Contact: For operator and technical assistance on this flow cytometer, please contact Jane Gordon at Jane.Gordon@ucsf.edu or Sarah.Elmes@ucsf.edu

Rates:
Self-Operated: $48.61/hour (internal rate) or $85.75/hour (external rate)
With Operator Assistance: $143.25/hour (internal rate) or $252.69 (external rate)


Sony SH800

Sony SH800The benchtop Sony SH800 cell sorter permits sorting of a wide range of cell sizes and applications using the 70 μm, 100 μm, and 130 μm microfluidics sorting chips.  This novel, chip-based design is fully integrated with comprehensive fluidics controls and advanced automation for set-up, acquisition, sort and analysis to make sorting less subjective, more precise and easier to use.  System software is intuitive and supports sorting into tubes and 96- and 384- well plates. 

Location: HD335, Helen Diller Building, Mission Bay Campus

Contact: For operator and technical assistance on this cell sorter, please contact Sarah Elmes at Sarah.Elmes@ucsf.edu or Jane Gordon at Jane.Gordon@ucsf.edu

Rates:
Self-Operated: $56.55/hour (internal rate) or $99.76/hour (external rate)
With Operator Assistance: $151.19/hour (internal rate) or $266.70 (external rate)

Sony SH800


FlowJo

FlowJo from Tree Star is designed for viewing and analyzing flow data. It provides a very fast method of post-acquisition manipulation of sample data that can be applied to all samples in a data set instantly. Advanced data presentation tools are available. Post-acquisition, multi-channel compensation for spectral spillover between parameters is also available.

Location: Lab 337, Helen Diller Building, Mission Bay Campus

Contact: For operator and technical assistance on this analysis software, please contact Jane Gordon at Jane.Gordon@ucsf.edu

Rates:
Self-Operated: $8.24/hour (internal rate) or $14.54/hour (external rate)