Research Summary

William Hyun is an expert in the field of cytometry, specialized in the technical areas of flow cytometry and cell sorting, confocal and digital microscopy, high-content and high-throughput screening, in vivo small animal imaging, encoded array systems, biomarkers and bioinformatics. Hyun has worked in the field of cytometry for more than twenty-five (25) years, nineteen (19) years as Director of the Laboratory for Cell Analysis (LCA). In 2014, Hyun initiated and was appointed Director of the Laboratory for Advanced Cytometry (LAC) and Director of the Sony Biotechnology Center in the Department of Laboratory Medicine. Hyun has past appointments in the Biomedical Sciences (BMS) and Tetrad Graduate Programs, Eli and Edythe Broad Center for Regenerative Medicine and Stem Cell Research and Affiliate Scientist appointments at Lawrence Berkeley National Lab (LBNL) and UC Berkeley (UCB). The LAC and Sony Biotechnology Center are focused on translating research science into the clinical cytology and pathology labs. Technology translation includes assay validation, development of CLIA/FDA approval processes, QC/QA procedures, automation and robotics, reimbursement coding, data handling, multiparametric data analysis and advanced presentation of results.
Other technology areas include development of nanochips for soluble microanalysis and microfluidic chips for genomic and cytometric assays. The first effort in cellular bar coding resulted in the formation of a commercial company, Vitra Biosciences. Other efforts include encoded micro-fabricated chips, commercialized by True Materials, a startup housed in the UCSF QB3 Incubator and eventually purchased by Affymetrix. Another program has been investigating the use of bioscaffolds and molecular assembly along with 3D bio-printing for use in cytometric assays for stem cell engraftment, directional cell growth, surgical repair, and organoids for high content imaging assays like cellular invasion, migration and maturation studies. Some of this work is now commercialized by Fibralign Corporation. Working with the UCSF Abate lab, new partitioning technologies are being implemented for refined multiplex genomic and proteomic analysis at the single cell and single molecule level. Three commercial companies, Mission Bio, Scribe Bio and Fluent Biosciences, were founded to commercialize UCSF IP. Other developmental projects include mass spec flow and mass image cytometry, buoyancy bubble-based cell isolation (Akadeum Bio), single cell Western blot technology from the Herr lab at UC Berkeley (Zephyrus Bio, now BioTechne), and clinical single cell genomic diagnostics for oncology/pathology.
Hyun is an angel investor/co-founder in several biotech startup companies, and currently sits on the Board of Mission Bio, Scribe Bio, Newbridge Media, Akadeum Biosciences, Fluent Bio and the Scientific Advisory Board of Fibralign Corporation, Intabio, and CellFe. Hyun is a venture partner at Genoa Ventures focused on early stage investments where biology meets technology. Hyun has current and past compensated consulting roles with ZEISS and CRI in the area of spectral microscopy, Becton Dickinson, Sony and Danaher Corporations in the area of flow cytometry and cell sorting, Bristol Meyer Squibb and Sanofi-Aventis/Regeneron in the area of high content screening. Past positions include investor/advisor to Accuri Cytometers, Vitra Biosciences, KAIROS, SEQ, True Materials, Biotrue, Symbiosis Biowares, and Unihart Biotech Pharma.
Hyun has authored over 100 peer-reviewed publications.

Honors & Awards

  • 1981
    National Science Foundation Special Achievement Award

Selected Publications

  • Sullam PM; Hyun W; Szollosi J; Dong JF; Foss WM; Lopez JA. Physical Proximity and Functional Interplay on the Platelet Plasma Membrane of the Glycoprotein Ib-IX-V Complex and the Fc Receptor, FcγRIIA. J. Biol. Chem, 273(9), 1998: 5331-5336.
  • Pulliam L; Stubblebine, M; Hyun W. Quantification of Neurotoxicity and Identification of Cellular Subsets in a Three Dimensional Brain Model. Cytometry, Volume 32,1998: 66-69.
  • Fung J; Hyun W; Dandekar P; Pedersen R; Weier HU. Spectral Imaging in Preconception/Preimplantation Genetic Diagnosis of Aneuploidy: Multicolor, Multichromosome Screening of Single Cells. J of Assisted Reproduction and Genetics, Volume 15:5,1998: 386-93.
  • Ryder MI; Fujitaki R; Lebus S; Mahboub M; Faia B; Muhaimin D; Hamada M; Hyun W. Alterations of neutrophil l-selectin and CD18 expression by tobacco smoke: implications for periodontal disease. Periodontal Res, Vol 33,1998.
  • Ryder MI, Fujitaki R, Johnson G, Hyun W. Alterations of neutrophil oxidative burst by in vitro smoke exposure: implications for oral and systemic diseases. Annals Periodontology 1998 Jul;3(1):76-87
  • Nagy P, Bene L, Balazs M, Hyun WC, Lockett SJ, Chiang NY, Waldman F, Feuerstein BG, Damjanovich S, Szollosi J EGF-induced redistribution of erbB2 on breast tumor cells: flow and image cytometric energy transfer measurements. Cytometry 1998 Jun 1;32(2):120-31
  • Stull RA; Hyun W; Pallavicini MG. Simultaneous Flow Cytometric Analyses of Enhanced Green and Yellow Fluorescent Proteins and Cell Surface Antigens in Transduced Hematopoietic Stem Cell Populations. Cytometry, Volume 40,2000: 126-34.
  • Chang JW, Peng M, Vaquerano JE, Zhou YM, Clinton RA, Hyun WC, Giedlin MA, Leong SP. Induction of Th1 response by dendritic cells pulsed with autologous melanoma apoptotic bodies. Anticancer Res;20(3A) 2000:1329-36.
  • Kobayashi A, Darragh T, et al, Hyun W, Smith-McCune K. Lymphoid Follicles are Generated in High Grade Cervical Dysplasia and Have Differing Characteristics Depending on HIV Status. Amer Journal Pathology. Vol 160:1, 2002:151.
  • Liu W, Enright J, Hyun W, Day R, Schaufele F. CCAAT/Enhancer Binding Protein alpha uses distinct domains to prolong pituitary cells in Growth 1 and DNA Synthesis phases of the cell cycle. BMC Cell Bio, 2002, 3(1):6.
  • Ryder MI, Wu TC, Kallaos SS, Hyun W. Alterations of neutrophil f-actin kinetics by tobacco smoke: implications for periodontal disease. J Periodontol Res 2002; 37; 286-292.
  • Foo NC, Ahn BY, Ma X, Hyun W, Yen TS. Cellular vacuolization and apoptosis induced by hepatitis B virus large surface protein. Hepatology 2002; Dec;36(6): 1400-7.
  • Park JW, Yeh M, et al, Hyun W, Clark OH. Heat Shock Protein 90-Binding Geldanamycin Inhibits Cancer Cell Proliferation, Down Regulates Oncoproteins, and Inhibits Epidermal Growth Factor-Induced Invasion in Thyroid Cancer Cell Lines.. Jour Clin Endocrinology & Metabolism, 2003, 88(7).
  • Limoli CL, Giedzinski E, et al, and Hyun W. Persistant Oxidative Stress in Chromosomally Unstable Cells. Cancer Res 63(12), Jun 15, 2003.
  • Park J, Wong M, Lobo M, Hyun W, Duh Q and Clark O. Modulation of TNF-Related Thyroid Cancer Cell Lines. Thyroid 13(12), 2004
  • Huang G, Krig S...Hyun W, Volik S, Feuerstein B, Yaswen P, Collins C. ZNF217 suppresses cell death associated with chemotherapy and telomere dysfunction. Cancer, 2004.
  • Nagy P, Bene L, Hyun W,...Park JW, Szollsi J. Novel calibration method for flow cytometric fluorescence resonance energy transfer measurements. Cytometry Oct;67(2):86-96, 2005.
  • Park JW, Hyun WC, Ginzinger DG, Lobo M, Cotter P, Duh QY, Clark OH. Troglitazone, the peroxisome proliferator-activated receptor-gamma agonist, induces antiproliferation and redifferentiation in human thyroid cancer cell lines. Thyroid. Mar;15(3):222-31, 2005.
  • Rubenstein J, Fridlyand J, Shen A, Aldape K,Hyun W, Parikh S, Haqq C, Shuman M. Gene expression and angiotropism in primary CNS lymphoma. Blood. 2006.

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