University of California San Francisco
Helen Diller Family Comprehensive Cancer Center
David P. Toczyski, PhD

David P. Toczyski, PhD

Professor, Department of Biochemistry and Biophysics, UCSF

Cancer Center Program Memberships



State University of New York/Buffalo, B.S., 1987, Biology
Yale University, M.Phil., 1990, Biophysics & Biochem
Yale University, Ph.D., 1992, Biophysics & Biochem

Professional Experience

  • 1984-1986
    State University New York - Buffalo, Undergrad Research
  • 1987-1992
    Yale University, Graduate Student
  • 1993-1998
    University of Washington/FHCRC, Postdoctoral Associate
  • 1998-2005
    UCSF Biochemistry & Biophysics/CRI, Assistant Professor
  • 2005-2010
    UCSF Biochemistry & Biophysics/CRI, Associate Professor
  • 2010-present
    UCSF Biochemistry & Biophysics/CRI,  Professor

Honors & Awards

  • 1993-1996
    Jane Coffin Childs Fellow
  • 2003-2009
    Leukemia and Lymphoma Society Scholar
  • 2005-present
    NDT study section, permanent member
  • 2009
    Keynote address, NIH Cell Cycle meeting

Selected Publications

  1. Alme EB, Stevenson E, Krogan NJ, Swaney DL, Toczyski DP. The kinase Isr1 negatively regulates hexosamine biosynthesis in S. cerevisiae. PLoS Genet. 2020 Jun 24; 16(6):e1008840.
    View on PubMed
  2. Sanvisens Delgado N, Toczyski DP. Mck1 kinase is a new player in the DNA damage checkpoint pathway. PLoS Genet. 2019 10; 15(10):e1008372.
    View on PubMed
  3. Meza-Gutierrez F, Simsek D, Toczyski DP. A genetic approach to study polyubiquitination in Saccharomyces cerevisiae. Methods Enzymol. 2019; 618:49-72.
    View on PubMed
  4. Meza Gutierrez F, Simsek D, Mizrak A, Deutschbauer A, Braberg H, Johnson J, Xu J, Shales M, Nguyen M, Tamse-Kuehn R, Palm C, Steinmetz LM, Krogan NJ, Toczyski DP. Genetic analysis reveals functions of atypical polyubiquitin chains. Elife. 2018 12 14; 7.
    View on PubMed
  5. Lao JP, Ulrich KM, Johnson JR, Newton BW, Vashisht AA, Wohlschlegel JA, Krogan NJ, Toczyski DP. The Yeast DNA Damage Checkpoint Kinase Rad53 Targets the Exoribonuclease, Xrn1. G3 (Bethesda). 2018 12 10; 8(12):3931-3944.
    View on PubMed
  6. van Emden TS, Forn M, Forné I, Sarkadi Z, Capella M, Martín Caballero L, Fischer-Burkart S, Brönner C, Simonetta M, Toczyski D, Halic M, Imhof A, Braun S. Shelterin and subtelomeric DNA sequences control nucleosome maintenance and genome stability. EMBO Rep. 2019 01; 20(1).
    View on PubMed
  7. Romero AM, Martínez-Pastor M, Du G, Solé C, Carlos M, Vergara SV, Sanvisens N, Wohlschlegel JA, Toczyski DP, Posas F, de Nadal E, Martínez-Pastor MT, Thiele DJ, Puig S. Phosphorylation and Proteasome Recognition of the mRNA-Binding Protein Cth2 Facilitates Yeast Adaptation to Iron Deficiency. mBio. 2018 09 18; 9(5).
    View on PubMed
  8. Mark KG, Loveless TB, Toczyski DP. Isolation of ubiquitinated substrates by tandem affinity purification of E3 ligase-polyubiquitin-binding domain fusions (ligase traps). Nat Protoc. 2016 Feb; 11(2):291-301.
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  9. Meza-Gutierrez F, Hundley FV, Toczyski DP. Parallel Parkin: Cdc20 Takes a New Partner. Mol Cell. 2015 Oct 01; 60(1):3-4.
    View on PubMed
  10. Mark KG, Meza-Gutierrez F, Johnson JR, Newton BW, Krogan NJ, Toczyski DP. Prb1 Protease Activity Is Required for Its Recognition by the F-Box Protein Saf1. Biochemistry. 2015 Jul 28; 54(29):4423-6.
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  11. Loveless TB, Topacio BR, Vashisht AA, Galaang S, Ulrich KM, Young BD, Wohlschlegel JA, Toczyski DP. DNA Damage Regulates Translation through ß-TRCP Targeting of CReP. PLoS Genet. 2015 Jun; 11(6):e1005292.
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  12. Edenberg ER, Mark KG, Toczyski DP. Ndd1 turnover by SCF(Grr1) is inhibited by the DNA damage checkpoint in Saccharomyces cerevisiae. PLoS Genet. 2015 Apr; 11(4):e1005162.
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  13. Downey M, Johnson JR, Davey NE, Newton BW, Johnson TL, Galaang S, Seller CA, Krogan N, Toczyski DP. Acetylome profiling reveals overlap in the regulation of diverse processes by sirtuins, gcn5, and esa1. Mol Cell Proteomics. 2015 Jan; 14(1):162-76.
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  14. Edenberg ER, Downey M, Toczyski D. Polymerase stalling during replication, transcription and translation. Curr Biol. 2014 May 19; 24(10):R445-52.
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  15. Edenberg ER, Vashisht AA, Topacio BR, Wohlschlegel JA, Toczyski DP. Hst3 is turned over by a replication stress-responsive SCF(Cdc4) phospho-degron. Proc Natl Acad Sci U S A. 2014 Apr 22; 111(16):5962-7.
    View on PubMed
  16. Mark KG, Simonetta M, Maiolica A, Seller CA, Toczyski DP. Ubiquitin ligase trapping identifies an SCF(Saf1) pathway targeting unprocessed vacuolar/lysosomal proteins. Mol Cell. 2014 Jan 09; 53(1):148-61.
    View on PubMed
  17. Edenberg ER, Vashisht A, Benanti JA, Wohlschlegel J, Toczyski DP. Rad53 downregulates mitotic gene transcription by inhibiting the transcriptional activator Ndd1. Mol Cell Biol. 2014 Feb; 34(4):725-38.
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  18. Downey M, Knight B, Vashisht AA, Seller CA, Wohlschlegel JA, Shore D, Toczyski DP. Gcn5 and sirtuins regulate acetylation of the ribosomal protein transcription factor Ifh1. Curr Biol. 2013 Sep 09; 23(17):1638-48.
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  19. Landry BD, Doyle JP, Toczyski DP, Benanti JA. F-box protein specificity for g1 cyclins is dictated by subcellular localization. PLoS Genet. 2012; 8(7):e1002851.
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  20. Foe IT, Toczyski DP. Ubiquitin ligases: Taming the APC. Nat Chem Biol. 2012 Mar 16; 8(4):323-4.
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