The research interests of my lab focus on defining the roles and the mechanisms of enzymes in complex biological processes and on developing technologies to facilitate these studies. Information on my lab can be found at the following website: http://www.craiklab.ucsf.edu/. The primary emphasis of our work has been on enzymes, with a particular emphasis on macromolecular recognition. Our original protein engineering studies have evolved to encompass various proteases as well as their endogenous inhibitors and membrane bound receptors, including uPAR, and have recently been successful at developing functional imaging probes for breast, colon, and prostate cancer using novel technologies. Our antibody engineering studies have identified Fabs that recognize conformational states of the target enzyme or receptor and are being developed into functional probes for in vivo imaging. The methodologies we developed to identify these functional Fabs have also been applied to enzymes and membrane proteins for structural and functional studies.
July 1, 2002 - June 30, 2022 - Research Training in Chemistry and Chemical Biology, Principal Investigator. Sponsor: NIH/NIGMS, Sponsor Award ID: T32GM064337
August 1, 2015 - July 31, 2020 - Mapping the conformational cycle of transmembrane transporters, Co-Investigator. Sponsor: NIH/NIGMS, Sponsor Award ID: P01GM111126
September 24, 2014 - August 31, 2019 - Antibody Research Technology Center, Co-Investigator. Sponsor: NIH/NCI, Sponsor Award ID: P41CA196276
July 10, 2013 - March 31, 2017 - Allosteric Inhibition of a Family of Proteolytic Enzymes, Principal Investigator. Sponsor: NIH/NIGMS, Sponsor Award ID: R01GM104659
Allegheny College, Meadville, PA, B.S., 1976, Chemistry
Columbia University, N.Y., NY, M.A., 1978, Chemistry
Columbia University, N.Y., NY, Ph.D., 1981, Chemistry