Research Summary

The focus of my research group is the protein homeostasis network, a network of over 1,000 different factors in human cells that maintain the proteome in a functional and balanced state. Our goal is to understand how this network functions, and how it is challenged and rewired in disease states, in particular cancer and neurodegenerative diseases. Our functional genomics platform enables us to elucidate dynamic networks and to pinpoint nodes that are potential therapeutic targets. Using biochemistry, biophysics and cell biology, we then "zoom in" on individual nodes of the network to reveal their mechanism of action.

As a postdoctoral scholar in Jonathan Weissman’s lab at UCSF, I spearheaded the development of a functional genomics platform for robust genome-wide screens and genetic interaction maps in mammalian cells (see section C.2 below). Very recently, we have enhanced this platform by developing CRISPR-based technologies enabling both loss-of-function and gain-of-function screens in mammalian cells (see section C.3 below). I am confident that our functional genomics approach will have a particularly high impact in its application to elucidate cancer-relevant cellular pathways. We have successfully used this approach to identify cellular factors that control the sensitivity of cancer cells to drugs (see section C.4 below). This approach can identify biomarkers predictive of clinical responses, predict possible mechanisms of drug resistance, and guide the development of combination therapies.

Research Funding

  • September 5, 2018 - June 30, 2023 - The Psychiatric Cell Map Initiative: Connecting Genomics, Subcellular Networks, and Higher Order Phenotypes, Co-Principal Investigator. Sponsor: NIH/NIMH, Sponsor Award ID: U01MH115747
  • September 30, 2018 - May 31, 2023 - Systematic elucidation of endosomal trafficking as a therapeutic opportunity in AD using CRISPR-based functional genomics, Principal Investigator. Sponsor: NIH/NIA, Sponsor Award ID: R01AG062359
  • September 30, 2016 - August 31, 2021 - Linking tau proteostasis with neuronal activity in FTD, Co-Investigator. Sponsor: NIH/NINDS, Sponsor Award ID: U54NS100717
  • September 30, 2015 - May 31, 2020 - Rewiring of the human protein homeostasis network in normal and disease contexts, Principal Investigator. Sponsor: NIH/NIGMS, Sponsor Award ID: DP2GM119139

Education

Marburg University, Germany, Pre-diploma, 06/00, Human Biology
Cambridge University, UK, B.A. (M.A.), First Class Honours, 06/03, Biochemistry
The Rockefeller University, Ph.D., 06/09, Biological Sciences (Cell Biology, Biophysics)
University of California, San Francisco, Postdoctoral Fellow, 07/15, Systems Biology

Honors & Awards

  • 2017
    Named to first cohort of Chan Zuckerberg Biohub Investigators
  • 2016
    Innovative Research Grant, Stand Up To Cancer
  • 2015
    New Innovator Award, NIH
  • 2015
    Allen Distinguished Investigator Award, Paul G. Allen Family Foundation
  • 2014
    Pathway to Independence Award (K99/R00), NIH
  • 2012
    Finalist, Deloitte QB3 Award for Innovation, California Institute for Quantitative Biosciences
  • 2012
    PBBR Postdoctoral Research Fellowship, Program for Breakthrough Biomedical Research, UCSF
  • 2010
    Postdoctoral Fellowship, Jane Coffin Childs Memorial Fund
  • 2006
    David Rockefeller Fellowship, The Rockefeller University
  • 2003
    Predoctoral Fellowship, Howard Hughes Medical Institute

Selected Publications

  1. Nuñez JK, Chen J, Pommier GC, Cogan JZ, Replogle JM, Adriaens C, Ramadoss GN, Shi Q, Hung KL, Samelson AJ, Pogson AN, Kim JYS, Chung A, Leonetti MD, Chang HY, Kampmann M, Bernstein BE, Hovestadt V, Gilbert LA, Weissman JS Genome-wide programmable transcriptional memory by CRISPR-based epigenome editing.  View on PubMed
  2. Ehrenberg AJ, Leng K, Letourneau KN, Hernandez I, Lew C, Seeley WW, Spina S, Miller B, Heinsen H, Kampmann M, Kosik KS, Grinberg LT Patterns of neuronal Rhes as a novel hallmark of tauopathies.  View on PubMed
  3. Abrams J, Arhar T, Mok SA, Taylor IR, Kampmann M, Gestwicki JE Functional genomics screen identifies proteostasis targets that modulate prion protein (PrP) stability.  View on PubMed
  4. Kanfer G, Sarraf SA, Maman Y, Baldwin H, Dominguez-Martin E, Johnson KR, Ward ME, Kampmann M, Lippincott-Schwartz J, Youle RJ Image-based pooled whole-genome CRISPRi screening for subcellular phenotypes.  View on PubMed
  5. Tian R, Samelson AJ, Rezelj VV, Chen M, Ramadoss GN, Guo X, Kain AM, Tran QD, Lim SA, Lui I, Nunez J, Rockwood SJ, Liu N, Carlson-Stevermer J, Oki J, Maures T, Holden K, Weissman JS, Wells JA, Conklin B, Vignuzzi M, Kampmann M BRD2 inhibition blocks SARS-CoV-2 infection in vitro by reducing transcription of the host cell receptor ACE2.  View on PubMed
  6. Leng K, Li E, Eser R, Piergies A, Sit R, Tan M, Neff N, Li SH, Rodriguez RD, Suemoto CK, Leite REP, Ehrenberg AJ, Pasqualucci CA, Seeley WW, Spina S, Heinsen H, Grinberg LT, Kampmann M Molecular characterization of selectively vulnerable neurons in Alzheimer's disease.  View on PubMed
  7. Semesta KM, Tian R, Kampmann M, von Zastrow M, Tsvetanova NG A high-throughput CRISPR interference screen for dissecting functional regulators of GPCR/cAMP signaling.  View on PubMed
  8. Bennett NK, Nguyen MK, Darch MA, Nakaoka HJ, Cousineau D, Ten Hoeve J, Graeber TG, Schuelke M, Maltepe E, Kampmann M, Mendelsohn BA, Nakamura JL, Nakamura K Defining the ATPome reveals cross-optimization of metabolic pathways.  View on PubMed
  9. Newberry RW, Arhar T, Costello J, Hartoularos GC, Maxwell AM, Naing ZZC, Pittman M, Reddy NR, Schwarz DMC, Wassarman DR, Wu TS, Barrero D, Caggiano C, Catching A, Cavazos TB, Estes LS, Faust B, Fink EA, Goldman MA, Gomez YK, Gordon MG, Gunsalus LM, Hoppe N, Jaime-Garza M, Johnson MC, Jones MG, Kung AF, Lopez KE, Lumpe J, Martyn C, McCarthy EE, Miller-Vedam LE, Navarro EJ, Palar A, Pellegrino J, Saylor W, Stephens CA, Strickland J, Torosyan H, Wankowicz SA, Wong DR, Wong G, Redding S, Chow ED, DeGrado WF, Kampmann M Robust Sequence Determinants of a-Synuclein Toxicity in Yeast Implicate Membrane Binding.  View on PubMed
  10. Ramkumar P, Abarientos AB, Tian R, Seyler M, Leong JT, Chen M, Choudhry P, Hechler T, Shah N, Wong SW, Martin TG, Wolf JL, Roybal KT, Pahl A, Taunton J, Wiita AP, Kampmann M CRISPR-based screens uncover determinants of immunotherapy response in multiple myeloma.  View on PubMed
  11. Kampmann M CRISPR-based functional genomics for neurological disease.  View on PubMed
  12. Jost M, Chen Y, Gilbert LA, Horlbeck MA, Krenning L, Menchon G, Rai A, Cho MY, Stern JJ, Prota AE, Kampmann M, Akhmanova A, Steinmetz MO, Tanenbaum ME, Weissman JS Pharmaceutical-Grade Rigosertib Is a Microtubule-Destabilizing Agent.  View on PubMed
  13. Khairunnisa Mentari Semesta, Ruilin Tian, Martin Kampmann, Mark von Zastrow, Nikoleta G. Tsvetanova A High-throughput CRISPR Interference Screen to Dissect Functional Regulators of GPCR/cAMP Signaling.  View on PubMed
  14. Rauch JN, Luna G, Guzman E, Audouard M, Challis C, Sibih YE, Leshuk C, Hernandez I, Wegmann S, Hyman BT, Gradinaru V, Kampmann M, Kosik KS LRP1 is a master regulator of tau uptake and spread.  View on PubMed
  15. Newberry RW, Leong JT, Chow ED, Kampmann M, DeGrado WF Deep mutational scanning reveals the structural basis for a-synuclein activity.  View on PubMed
  16. Guo X, Aviles G, Liu Y, Tian R, Unger BA, Lin YT, Wiita AP, Xu K, Correia MA, Kampmann M Mitochondrial stress is relayed to the cytosol by an OMA1-DELE1-HRI pathway.  View on PubMed
  17. Oh E, Mark KG, Mocciaro A, Watson ER, Prabu JR, Cha DD, Kampmann M, Gamarra N, Zhou CY, Rape M Gene expression and cell identity controlled by anaphase-promoting complex.  View on PubMed
  18. Chen JJ, Nathaniel DL, Raghavan P, Nelson M, Tian R, Tse E, Hong JY, See SK, Mok SA, Hein MY, Southworth DR, Grinberg LT, Gestwicki JE, Leonetti MD, Kampmann M Compromised function of the ESCRT pathway promotes endolysosomal escape of tau seeds and propagation of tau aggregation.  View on PubMed
  19. Uckeley ZM, Moeller R, Kühn LI, Nilsson E, Robens C, Lasswitz L, Lindqvist R, Lenman A, Passos V, Voss Y, Sommerauer C, Kampmann M, Goffinet C, Meissner F, Överby AK, Lozach PY, Gerold G Quantitative Proteomics of Uukuniemi Virus-host Cell Interactions Reveals GBF1 as Proviral Host Factor for Phleboviruses.  View on PubMed
  20. Tian R, Gachechiladze MA, Ludwig CH, Laurie MT, Hong JY, Nathaniel D, Prabhu AV, Fernandopulle MS, Patel R, Abshari M, Ward ME, Kampmann M CRISPR Interference-Based Platform for Multimodal Genetic Screens in Human iPSC-Derived Neurons.  View on PubMed

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