Research Summary

My work focuses on a fundamental biological problem: understanding the regulatory system that guides the eukaryotic cell through the stages of the cell division cycle. My laboratory studies this problem primarily in the budding yeast Saccharomyces cerevisiae, but my findings have broad significance for human diseases, such as cancer, that arise from defects in cell proliferation or chromosome behavior. The research strategy of my laboratory is to use quantitative biochemical analysis to understand the detailed mechanisms of key enzymes involved in cell cycle control. We also use molecular genetics, proteomics, advanced light microscopy, and computational methods to explore how these enzymes are assembled into a robust regulatory system that drives accurate cell cycle progression and chromosome segregation.

Research Funding

  • July 1, 1979 - June 30, 2021 - Cell Biology, Genetics, and Biochemistry Training Grant, Principal Investigator. Sponsor: NIH/NIGMS, Sponsor Award ID: T32GM007810
  • May 1, 2016 - April 30, 2021 - Regulatory Enzymes and Systems in Cell Cycle Control, Principal Investigator. Sponsor: NIH/NIGMS, Sponsor Award ID: R35GM118053
  • August 3, 1990 - August 31, 2016 - Molecular Control of Cell Proliferation, Principal Investigator. Sponsor: NIH/NIGMS, Sponsor Award ID: R37GM053270
  • January 1, 2004 - March 31, 2016 - Cell Cycle Control by Cyclin-Dependent Kinases, Principal Investigator. Sponsor: NIH/NIGMS, Sponsor Award ID: R01GM069901

Education

University of Calgary, Canada, B.Sc. (Hon.), 1980, Animal Biology
University of California San Francisco, Ph.D., 1986, Endocrinology
University of California San Francisco, Post-Doc, 1986-1989, Biochemistry

Honors & Awards

  • 1987-1989
    Helen Hay Whitney Foundation Postdoctoral Fellowship
  • 1990-1993
    Searle Scholar Award
  • 1990-1992
    March of Dimes Basil O'Connor Starter Scholar Award
  • 1991-1996
    Rita Allen Foundation Scholar Award
  • 2007-present
    Jack D. and DeLoris Lange Endowed Chair in Physiology
  • 1997, 2003, 2006, 2008, 2010, 2012
    UCSF Medical School Teaching Award for Outstanding Lecture Series
  • 2010
    UCSF Kaiser Award for Excellence in Teaching in the Classroom Setting
  • 2010
    UCSF Graduate Students Association Outstanding Faculty Mentorship Award
  • 2011
    MERIT award, NIGMS
  • 2012
    Fellow, Royal Society of London

Selected Publications

  1. Carlson CR, Asfaha JB, Ghent CM, Howard CJ, Hartooni N, Safari M, Frankel AD, Morgan DO Phosphoregulation of Phase Separation by the SARS-CoV-2 N Protein Suggests a Biophysical Basis for its Dual Functions.  View on PubMed
  2. Mizrak A, Morgan DO Polyanions provide selective control of APC/C interactions with the activator subunit.  View on PubMed
  3. Rosen LE, Klebba JE, Asfaha JB, Ghent CM, Campbell MG, Cheng Y, Morgan DO Cohesin cleavage by separase is enhanced by a substrate motif distinct from the cleavage site.  View on PubMed
  4. Qin L, Mizrak A, Guimarães DSPSF, Tambrin HM, Morgan DO, Hall MC The pseudosubstrate inhibitor Acm1 inhibits the anaphase-promoting complex/cyclosome by combining high-affinity activator binding with disruption of Doc1/Apc10 function.  View on PubMed
  5. Seoane AI, Morgan DO Firing of Replication Origins Frees Dbf4-Cdc7 to Target Eco1 for Destruction.  View on PubMed
  6. Davey NE, Morgan DO Building a Regulatory Network with Short Linear Sequence Motifs: Lessons from the Degrons of the Anaphase-Promoting Complex.  View on PubMed
  7. Lu D, Girard JR, Li W, Mizrak A, Morgan DO Quantitative framework for ordered degradation of APC/C substrates.  View on PubMed
  8. Girard JR, Tenthorey JL, Morgan DO An E2 accessory domain increases affinity for the anaphase-promoting complex and ensures E2 competition.  View on PubMed
  9. Lu D, Hsiao JY, Davey NE, Van Voorhis VA, Foster SA, Tang C, Morgan DO Multiple mechanisms determine the order of APC/C substrate degradation in mitosis.  View on PubMed
  10. Eshleman HD, Morgan DO Sgo1 recruits PP2A to chromosomes to ensure sister chromatid bi-orientation during mitosis.  View on PubMed
  11. Van Voorhis VA, Morgan DO Activation of the APC/C ubiquitin ligase by enhanced E2 efficiency.  View on PubMed
  12. Naylor SG, Morgan DO Cdk1-dependent phosphorylation of Iqg1 governs actomyosin ring assembly prior to cytokinesis.  View on PubMed
  13. Morgan DO The D box meets its match.  View on PubMed
  14. Foster SA, Morgan DO The APC/C subunit Mnd2/Apc15 promotes Cdc20 autoubiquitination and spindle assembly checkpoint inactivation.  View on PubMed
  15. Yaakov G, Thorn K, Morgan DO Separase biosensor reveals that cohesin cleavage timing depends on phosphatase PP2A(Cdc55) regulation.  View on PubMed
  16. Foe IT, Foster SA, Cheung SK, DeLuca SZ, Morgan DO, Toczyski DP Ubiquitination of Cdc20 by the APC occurs through an intramolecular mechanism.  View on PubMed
  17. Schaefer JB, Morgan DO Protein-linked ubiquitin chain structure restricts activity of deubiquitinating enzymes.  View on PubMed
  18. Kõivomägi M, Valk E, Venta R, Iofik A, Lepiku M, Morgan DO, Loog M Dynamics of Cdk1 substrate specificity during the cell cycle.  View on PubMed
  19. Lyons NA, Morgan DO Cdk1-dependent destruction of Eco1 prevents cohesion establishment after S phase.  View on PubMed
  20. Rodrigo-Brenni MC, Foster SA, Morgan DO Catalysis of lysine 48-specific ubiquitin chain assembly by residues in E2 and ubiquitin.  View on PubMed

Go to UCSF Profiles, powered by CTSI