Research Summary

The goal of my laboratory is to better understand the biology of skin cancer. We employ a variety of -omic approaches, including the use of DNA-sequencing, RNA-sequencing, and single-cell sequencing technologies. Our main focus is on melanoma and cutaneous squamous cell carcinoma. Previously, we have defined the driver genes and the mutational processes that were operative in these types of cancer. We are also interested in their precursor lesions (common nevi and actinic keratoses) as well as their cells of origin (melanocytes and keratinocytes). By studying the early stages of tumorigenesis in addition to fully-evolved tumors, we have delineated the critical events involved in the progression of skin cancers from individual cells, to benign neoplasms, to invasive cancers, and eventually to metastases. Collectively, our work has improved the diagnosis and staging of skin cancers and offered important guidance for prevention campaigns.

Education

University of Illinois, Urbana/Champaign, IL, BS, 05/2006, Biochemistry
Stanford University, Palo Alto, CA PhD 04/2012 Cancer Biology
University of California San Francisco, San Francisco, CA, Postdoctoral Fellow, 09/2017

Honors & Awards

  • 2006
    Graduate Summa Cum Laude, University of Illinois
  • 2006
    William T. & Lynn Jackson Best Senior Thesis Award, University of Illinois
  • 2007
    Stanford Graduate Fellowship
  • 2008
    National Science Foundation Graduate Fellowship
  • 2013
    Ruth L Kirschstein National Service Award Postdoctoral Fellowship
  • 2016
    The NCI Transition Career Development Award (K22)
  • 2018
    Selected as one of the Next Generation Stars of Cancer Research by the American Association of Cancer Research
  • 2018
    Recipient of the Young Investigator Award by the Society of Melanoma Research

Selected Publications

  1. Chang D, Shain AH. The landscape of driver mutations in cutaneous squamous cell carcinoma. NPJ Genom Med. 2021 Jul 16; 6(1):61.  View on PubMed
  2. Shain AH. Two Trajectories to Melanoma on the Hands and Feet. JAMA Dermatol. 2021 Jul 01; 157(7):769-770.  View on PubMed
  3. Bowman RL, Hennessey RC, Weiss TJ, Tallman DA, Crawford ER, Murphy BM, Webb A, Zhang S, La Perle KM, Burd CJ, Levine RL, Shain AH, Burd CE. UVB mutagenesis differs in Nras- and Braf-mutant mouse models of melanoma. Life Sci Alliance. 2021 09; 4(9).  View on PubMed
  4. Tang J, Fewings E, Chang D, Zeng H, Liu S, Jorapur A, Belote RL, McNeal AS, Tan TM, Yeh I, Arron ST, Judson-Torres RL, Bastian BC, Shain AH. Publisher Correction: The genomic landscapes of individual melanocytes from human skin. Nature. 2021 Feb; 590(7844):E20.  View on PubMed
  5. Tang J, Fewings E, Chang D, Zeng H, Liu S, Jorapur A, Belote RL, McNeal AS, Tan TM, Yeh I, Arron ST, Judson-Torres RL, Bastian BC, Shain AH. The genomic landscapes of individual melanocytes from human skin. Nature. 2020 10; 586(7830):600-605.  View on PubMed
  6. Cao W, Lee H, Wu W, Zaman A, McCorkle S, Yan M, Chen J, Xing Q, Sinnott-Armstrong N, Xu H, Sailani MR, Tang W, Cui Y, Liu J, Guan H, Lv P, Sun X, Sun L, Han P, Lou Y, Chang J, Wang J, Gao Y, Guo J, Schenk G, Shain AH, Biddle FG, Collisson E, Snyder M, Bivona TG. Multi-faceted epigenetic dysregulation of gene expression promotes esophageal squamous cell carcinoma. Nat Commun. 2020 07 22; 11(1):3675.  View on PubMed
  7. Shain AH, Bastian BC. Author Correction: From melanocytes to melanomas. Nat Rev Cancer. 2020 Jun; 20(6):355.  View on PubMed
  8. Botton T, Talevich E, Mishra VK, Zhang T, Shain AH, Berquet C, Gagnon A, Judson RL, Ballotti R, Ribas A, Herlyn M, Rocchi S, Brown KM, Hayward NK, Yeh I, Bastian BC. Genetic Heterogeneity of BRAF Fusion Kinases in Melanoma Affects Drug Responses. Cell Rep. 2019 10 15; 29(3):573-588.e7.  View on PubMed
  9. Zeng H, Judson-Torres RL, Shain AH. The Evolution of Melanoma - Moving beyond Binary Models of Genetic Progression. J Invest Dermatol. 2020 02; 140(2):291-297.  View on PubMed
  10. Yeh I, Jorgenson E, Shen L, Xu M, North JP, Shain AH, Reuss D, Wu H, Robinson WA, Olshen A, von Deimling A, Kwok PY, Bastian BC, Asgari MM. Targeted Genomic Profiling of Acral Melanoma. J Natl Cancer Inst. 2019 10 01; 111(10):1068-1077.  View on PubMed
  11. Torres R, Lang UE, Hejna M, Shelton SJ, Joseph NM, Shain AH, Yeh I, Wei ML, Oldham MC, Bastian BC, Judson-Torres RL. MicroRNA Ratios Distinguish Melanomas from Nevi. J Invest Dermatol. 2020 01; 140(1):164-173.e7.  View on PubMed
  12. Shain AH, Bagger MM, Yu R, Chang D, Liu S, Vemula S, Weier JF, Wadt K, Heegaard S, Bastian BC, Kiilgaard JF. The genetic evolution of metastatic uveal melanoma. Nat Genet. 2019 07; 51(7):1123-1130.  View on PubMed
  13. Joseph NM, Tsokos CG, Umetsu SE, Shain AH, Kelley RK, Onodera C, Bowman S, Talevich E, Ferrell LD, Kakar S, Krings G. Genomic profiling of combined hepatocellular-cholangiocarcinoma reveals similar genetics to hepatocellular carcinoma. J Pathol. 2019 06; 248(2):164-178.  View on PubMed
  14. Webster JD, Pham TH, Wu X, Hughes NW, Li Z, Totpal K, Lee HJ, Calses PC, Chaurushiya MS, Stawiski EW, Modrusan Z, Chang MT, Tran C, Lee WP, Chalasani S, Hung J, Sharma N, Chan S, Hotzel K, Talevich E, Shain A, Xu M, Lill J, Dixit VM, Bastian BC, Dey A. The tumor suppressor BAP1 cooperates with BRAFV600E to promote tumor formation in cutaneous melanoma. Pigment Cell Melanoma Res. 2019 03; 32(2):269-279.  View on PubMed
  15. Zeng H, Jorapur A, Shain AH, Lang UE, Torres R, Zhang Y, McNeal AS, Botton T, Lin J, Donne M, Bastian IN, Yu R, North JP, Pincus L, Ruben BS, Joseph NM, Yeh I, Bastian BC, Judson RL. Bi-allelic Loss of CDKN2A Initiates Melanoma Invasion via BRN2 Activation. Cancer Cell. 2018 07 09; 34(1):56-68.e9.  View on PubMed
  16. Shain AH, Joseph NM, Yu R, Benhamida J, Liu S, Prow T, Ruben B, North J, Pincus L, Yeh I, Judson R, Bastian BC. Genomic and Transcriptomic Analysis Reveals Incremental Disruption of Key Signaling Pathways during Melanoma Evolution. Cancer Cell. 2018 07 09; 34(1):45-55.e4.  View on PubMed
  17. Shain AH, Bastian BC. Filling the gaps in the genomic catalogue of melanoma subtypes. Pigment Cell Melanoma Res. 2017 01; 30(6):508-509.  View on PubMed
  18. Yeh I, Lang UE, Durieux E, Tee MK, Jorapur A, Shain AH, Haddad V, Pissaloux D, Chen X, Cerroni L, Judson RL, LeBoit PE, McCalmont TH, Bastian BC, de la Fouchardière A. Combined activation of MAP kinase pathway and β-catenin signaling cause deep penetrating nevi. Nat Commun. 2017 09 21; 8(1):644.  View on PubMed
  19. Chiba K, Lorbeer FK, Shain AH, McSwiggen DT, Schruf E, Oh A, Ryu J, Darzacq X, Bastian BC, Hockemeyer D. Mutations in the promoter of the telomerase gene TERT contribute to tumorigenesis by a two-step mechanism. Science. 2017 09 29; 357(6358):1416-1420.  View on PubMed
  20. Yeh I, Tee MK, Botton T, Shain AH, Sparatta AJ, Gagnon A, Vemula SS, Garrido MC, Nakamaru K, Isoyama T, McCalmont TH, LeBoit PE, Bastian BC. NTRK3 kinase fusions in Spitz tumours. J Pathol. 2016 11; 240(3):282-290.  View on PubMed

Go to UCSF Profiles, powered by CTSI