Histology & Biomarkers

The Histology & Biomarker Core provides state-of-the-art tissue biomarker histologic detection and image analyses for both human and mouse tissue biospecimens. To order work, visit our iLab website.

Histology

  • Optimized processing of unfixed tissues for microtomy/biomarkers
  • Embedding
  • FFPE microtomy
  • Cryo-microtomy
  • RNase-free scrolls/sections
  • Hematoxylin & Eosin staining
  • Special histochemical stains
  • Tissue microarray construction
  • Cell button creation
  • Histology instruction & consultation

Biomarker Staining

Our laboratory offers a robust menu of immunohistochemistry (IHC) and immunofluorescence (IF) single and multiplex panel stains on the Ventana Discovery Ultra platform.

  • IHC/IF -- single & multiplex staining
  • Multiplex IHC chromogenic panels up to three markers
  • Multiplex IF panels up to 5 markers using Ventana’s staining technology or 6 markers using Akoya’s OPAL dyes
  • In situ hybridization (RNAscope)
  • Current list of protocols can be found here: Biomarker Menu

Whole Slide Scanning

Digital imaging services include both brightfield (BF) and immunofluorescent (IF) slides as well multispectral scanning.

  • Zeiss Axio ScanZ.1 - Whole Slide Scanning Service
    • Automated loading of up to 100 slides
    • 10x/20x/40x objective scanning for BF and FL 
    • 9 fluorescent channels: Cy7, Cy5, Red 610, Alexa 594/Texas Red/RFP, Cy3, FITC/GFP, DCC/Coumarin, Rhodamine 6G, DAPI
  • Akoya Vectra 3.0 - Rental
    • Automated loading of up to 6 slides
    • 5x, 10x, 20x, 40x objective scanning BF or FL (multi-spectral/color)
    • 5 filter cubes: FITC/AF488, Cy3/TITC/AF555, DAPI, AF594/Texas Red, Cy5/-ET

Image Processing

Our image analysis specialist offers strategic planning of biomarker panels and project-oriented quantitative analysis of H&E/IHC/IF images.

For more information please see our Imaging/Scanning FAQs