Biomarkers and Imaging

Biomarker Staining

Our laboratory offers a robust menu of immunohistochemistry (IHC) and immunofluorescence (IF) single and multiplex panel stains on the Ventana Discovery Ultra platform.

  • IHC/IF -- single & multiplex staining
  • Multiplex IHC chromogenic panels up to three markers
  • Multiplex IF panels up to 5 markers using Ventana’s staining technology or 6 markers using Akoya’s OPAL dyes
  • In situ hybridization (RNAscope)
  • Current list of protocols can be found here: Biomarker Menu

Whole Slide Scanning

Digital imaging services include both brightfield (BF) and immunofluorescent (IF) slides as well multispectral scanning.

  • Zeiss Axio ScanZ.1 - Whole Slide Scanning Service
    • Automated loading of up to 100 slides
    • 10x/20x/40x objective scanning for BF and FL 
    • 9 fluorescent channels: Cy7, Cy5, Red 610, Alexa 594/Texas Red/RFP, Cy3, FITC/GFP, DCC/Coumarin, Rhodamine 6G, DAPI

Image Processing

Our image analysis specialist offers strategic planning of biomarker panels and project-oriented quantitative analysis of H&E/IHC/IF images.

For more information please see our Imaging/Scanning FAQs


Additional Resources