Flow Cytometry & Cell Sorting Instruments and Services
The LCA provides access and training on three ThermoFisher ATTUNE NxT analyzers and one BD FACS CANTO benchtop cytometers with HTS, four BD FACS FUSION cell sorters, a BD S8 Spectral/Imaging cell sorter and one Sony SH800 cell sorter. These systems are primarily used for the rapid analysis and/or sorting of single-cell suspensions. To analyze the light scatter and fluorescence characteristics of a cell population, the benchtop machines are appropriate. To rapidly retrieve or sort a particular sub-group of cells from a mixed sample, a cell sorter would be required.
Instrument Operation/Training Rates:
Current hourly rates for instruments and services are available on the LCA i-Labs software.
For operator and technical assistance and core staff time, please contact Sarah Elmes at [email protected].
FACS FUSIONs
The BD FACS FUSION is a high-speed digital sorter that incorporates a fixed-alignment cuvette flow cell. Fiber optics direct and focus five lasers (360nm, 405nm, 488nm, 561nm, and 633nm) onto the alignment prisms and then focuses on the cuvette flow cell. The flow cell allows for improved light excitation and collection optics. The collection optics consist of octagon- and trigon-shaped collection devices, and a total of up to 18 independent signals can be acquired at one time on a single-cell basis, followed by purification of up to 4 cell subsets simultaneously by sorting into tubes or sort single cells into multi-well formats.
Location:
- Room HD337, Helen Diller Building, Mission Bay Campus (FUSION 1, FUSION 2 and FUSION 3)
- Room 559 Weill NeuroSciences Building, Mission Bay Campus (Weill FUSION)
Rates: Current hourly rates for instruments and services are available on the LCA i-Labs software.
Contact: For operator and technical assistance and core staff time, please contact Sarah Elmes at [email protected].
BD S8 Discover Spectral/Imaging Cell Sorter
The BD S8 is a state-of-the-art spectral based 5-laser cell sorter. The spectral aspect allows design panels of 30+ parameters. It can sort 6-ways simultaneously into tubes as well as into multi-well formats. The S8 is also capable of generating images of the cells via the 488nm laser. Physical and fluorescent characteristics can be used for gating populations for isolation.
Location: Room S-252C, Genentech Hall, Mission Bay Campus
Rates: Current hourly rates for instruments and services are available on the LCA i-Labs software.
Contact: For operator and technical assistance on this cell sorter, please contact Sarah Elmes at [email protected]
FACS Canto II
FACSCanto II from Becton Dickinson is designed for advanced analytical research applications using digital signal processing and flow cell optics, similar to the Aria II systems. The Canto II is equipped with the latest technical capabilities - high-speed electronics, multiple laser excitations across a wide spectral range (from 405 up to 640nm) and up to eighteen PMT detectors, and HTS 96 well plate reader.
Location: Room 512, Rock Hall, Mission Bay Campus
Contact: For operator and technical assistance on this flow cytometer, please contact Anna Celli at [email protected].
Rates: Current hourly rates for instruments and services are available on the LCA i-Labs software.
Attune NxT
The Attune NxT Flow Cytometer offers increased flexibility, intuitive software, and a high throughput 96 well platform for data acquisition with hydrodynamic focusing technology. A total of 14 fluorescent detection parameters along with Forward & Side scatter, and 4 excitation lasers including 488, 405, 561, and 637nm.
Location: HD344 and HD254 Helen Diller Building, Mission Bay Campus
Contact: For operator and technical assistance on this flow cytometer, please contact [email protected] or [email protected]
Rates: Current hourly rates for instruments and services are available on the LCA i-Labs software.
Sony SH800
The benchtop Sony SH800 cell sorter permits sorting of a wide range of cell sizes and applications using the 70 μm, 100 μm, and 130 μm microfluidics sorting chips. This novel, chip-based design is fully integrated with comprehensive fluidics controls and advanced automation for set-up, acquisition, sort and analysis to make sorting less subjective, more precise and easier to use. System software is intuitive and supports sorting into tubes and 96- and 384- well plates.
Location: HD344, Helen Diller Building, Mission Bay Campus
Contact: For operator and technical assistance on this cell sorter, please contact Sarah Elmes at [email protected] or Anna Celli at [email protected].
Rates: Current hourly rates for instruments and services are available on the LCA i-Labs software.
FlowJo
FlowJo from Tree Star is designed for viewing and analyzing flow data. It provides a very fast method of post-acquisition manipulation of sample data that can be applied to all samples in a data set instantly. Advanced data presentation tools are available. Post-acquisition, multi-channel compensation for spectral spillover between parameters is also available.
Location: HD561, Helen Diller Building, Mission Bay Campus
Contact: For operator and technical assistance on this analysis software, please contact Anna Celli at [email protected].
Rates: Current hourly rates for instruments and services are available on the LCA i-Labs software.